请使用支持JavaScript的浏览器!
主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Amplite™ Colorimetric Glucose Quantitation Kit/40004/500 Tests
产品编号:40004
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Amplite™ Colorimetric Glucose Quantitation Kit/40004/500 Tests
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)575/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategorySmallMoleculeDetection
DiagnosticMolecules
RelatedGlucoseTransporters
BiochemicalAssays
Glucose,amonosaccharide,isthemostimportantcarbohydrateinBIOLOGy.Itisasourceofenergyandmetabolicintermediateforcellgrowth.Asoneofthemainproductsofphotosynthesis,glucosestartscellularrespirationinbothprokaryotesandeukaryotes.Glucoselevelisakeydiagnosticparameterformanymetabolicdisorders,e.g.,diabetes.ThisAmplite™ColorimetricGlucoseQuantitationKitprovidesaquickandsensitivemethodforthemeasurementofglucose.Itusesglucoseoxidase-basedenzymecoupledreactionstodetectglucosethroughtheproductionofhydrogenperoxide,whichismonitoredbyourAmplite™Redperoxidasesubstrate.Amplite™Redperoxidasesubstratecanbereadbyanabsorbancemicroplatereaderat~570nm.Theassayisrobust,andcanbereADIlyadaptedforawidevarietyofapplicationsthatrequirethemeasurementofglucose.TheassayhasverylowbackgroundsinceitisrunintheredvisIBLerangethatsignificantlyreducestheinterferencefrombiologicalsamples.WiththeAmplite™ColorimetricGlucoseQuantitationKit,wecandetectaslittleas3?MD-glucose.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
Sorry,yourbrowserdoesnotsupportinlineSVG.Sorry,yourbrowserdoesnotsupportinlineSVG.
Movemouseovergridtodisplaywavelength&intensityvalues.

300
400
500
600
700
800
900
Wavelength(nm)


Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Preparestocksolutions:

1.1   250XAmplite™Redstocksolution:Add100µLofDMSO(ComponentE)intothevialofAmplite™Redsubstrate(ComponentA).Thestocksolutionshouldbeusedpromptly.Anyremainingsolutionshouldbealiquotedandrefrozenat-20oC.

Note1:Avoidrepeatedfreeze-thawcycles.

Note2:TheAmplite™Redsubstrateisunstableinthepresenceofthiolssuchasdithiothreitol(DTT)and2-mercaptoethanol.ThefinalconcentrationofDTTor2-mercaptoethanolinthereactionshouldbenohigherthan10μM.TheAmplite™RedsubstrateisalsounstableathighpH(>8.5).Therefore,thereactionshouldbeperformedatpH7–8.Theprovidedassaybuffer(pH7.4)isrecommended.

1.2   10U/mLHRPstocksolution:Add1mLofassaybuffer(ComponentB)intothevialofhorseradishperoxidase(ComponentC).

Note:TheunusedHRPsolutionshouldbedividedintosingleusealiquotsandstoredat-20oC.

1.3   100U/mLglucoseoxidasesolution:Add1mLofassaybuffer(ComponentB)intothevialofglucoseoxidase(ComponentD).

Note:Theunusedglucoseoxidasesolutionshouldbedividedintosingleusealiquotsandstoredat-20oC.

1.4   800mMglucosestocksolution:Add1mLofassaybuffer(ComponentB)intothevialofglucose(ComponentF).

Note:Theunusedglucosesolutionshouldbestoredat-20oC.

 

2.Prepareassayreactionmixture:

PrepareAssayreactionmixtureaccordingtothefollowingtables,protectedfromlight.

 

Table1Assayreactionmixtureforoneclearbottom96-wellmicroplate(2X)

Components

Volume

250XAmplite™RedStockSolution(fromStep1.1)

20μL

10U/mLHRPStockSolution(fromStep1.2)

100μL

100U/mLGlucoseOxidaseSolution(fromStep1.3)

100μL

AssayBuffer(ComponentB)

4.78mL

Totalvolume

5mL

 

Table2Layoutofglucosestandardsandtestsamplesinaclearbottom96-wellmicroplate

BL

BL

TS

TS

….

….

 

 

 

 

 

 

GS1

GS1

….

….

….

….

 

 

 

 

 

 

GS2

GS2

 

 

 

 

 

 

 

 

 

 

GS3

GS3

 

 

 

 

 

 

 

 

 

 

GS4

GS4

 

 

 

 

 

 

 

 

 

 

GS5

GS5

 

 

 

 

 

 

 

 

 

 

GS6

GS6

 

 

 

 

 

 

 

 

 

 

GS7

GS7

 

 

 

 

 

 

 

 

 

 

              Note:GS=Glucosestandards,BL=Blankcontrol,TS=testsamples.

Table3.Reagentcompositionforeachwell

GlucoseStandard

BlankControl

TestSample

SerialDilutions*:50μL

AssayBuffer(ComponentB):50μL

50μL

*Note:Addtheseriallydilutedglucosestandardsfromapproximately1.6µMto100µMintoeachwellfromGS1toGS7induplicate.

 

3.RunGlucoseassay:

3.1   Prepareaglucosestandardbydilutingtheappropriateamountofthe800mMglucosestocksolution(fromStep1.4)intoassaybuffer(ComponentB)toproduceglucoseconcentrationsof100μM.Thenperform1:2serialdilutionsinassaybuffer(ComponentB)togetapproximately50,25,12.5,6.3,3.1and1.6μMseriallydilutedglucosestandards.Anon-glucosebuffercontrolisincludedasblankcontrol.

3.2   Add50μLofassayreactionmixture(fromStep2)intoeachwellofglucosestandard,blankcontrol,andtestsamples(seeStep2,Table3)tomakethetotalglucoseassayvolumeof100µL/well

Note:Fora384-wellplate,add25μLofsampleand25μLofassayreactionmixtureintoeachwell.

3.3   Incubatethereactionfor10to30minutesat37oC,protectedfromlight.

3.4   MonitortheabsorbanceincreasewithanabsorbanceplatereaderatOD=570nm.

References&Citations
CitationExplorer

MicroRNAsregulategeneplasticityduringcoldshockinzebrafishlarvae
Authors:I-ChenHung,Yu-ChuanHsiao,HSunnySun,Tsung-MingChen,Shyh-JyeLee
Journal:BMCgenomics(2016):922

Glucosemetabolismandgeneexpressioninjuvenilezebrafish(Daniorerio)challengedwithahighcarbohydratediet:effectsofanacuteglucosestimulusduringlateembryoniclife
Authors:FilipaRocha,JorgeDias,SofiaEngrola,PauloGavaia,IngeGeurden,MariaTeresaDinis,StephanePanserat
Journal:BritishJournalofNutrition(2015):403--413

Glucoseoverloadinyolkhaslittleeffectonthelong-termmodulationofcarbohydratemetabolicgenesinzebrafish(Daniorerio)
Authors:FilipaRocha,JorgeDias,SofiaEngrola,PauloGavaia,IngeGeurden,MariaTeresaDinis,StephanePanserat
Journal:JournalofExperimentalBiology(2014):1139--1149

ImpactofL-FABPandglucoseonpolyunsaturatedfattyacidinductionofPPARα-regulatedβ-oxidativeenzymes
Authors:AncaDPetrescu,HuanHuang,GregoryGMartin,AveryLMcIntosh,StephenMStorey,DaniloLandrock,AnnBKier,FriedhelmSchroeder
Journal:AmericanJournalofPhysiology-GastrointestinalandLiverPhysiology(2013):G241--G256

InhibitorsoffattyacidsynthesisinducePPARα-regulatedfattyacidβ-oxidativegenes:synergisticrolesofL-FABPandglucose
Authors:HuanHuang,AveryLMcIntosh,GregoryGMartin,AncaDPetrescu,KerstinKLandrock,DaniloLandrock,AnnBKier,FriedhelmSchroeder
Journal:PPARresearch(2013)

Aquaporin-9proteinistheprimaryrouteofhepatocyteglyceroluptakeforglycerolgluconeogenesisinmice
Authors:SABInaJelen,SörenWacker,CamiloAponte-Santamaría,MartinSkott,AleksandraRojek,UrbanJohanson,PerKjellbom,SørenNielsen,BertLdeGroot,MichaelRützler
Journal:JournalofBiologicalChemistry(2011):44319--44325


品牌介绍

AAT Bioquest常用产品报价单

产品名货号公司分类商城分类美金价
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg2iFluor Rapid Tests酸碱缓冲液1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug8B0001Signaling Intermediate Ab重组抗体2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug8A0022Phospho-Specific Ab受体2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug16440Fluorescent Anti-IgGs荧光染料1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug16518Fluorescent Anti-IgGs抗小鼠4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug16900Streptavidin Conjugates其他生物染料1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug8C14708Signaling Intermediate Ab功能性抗体2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate12518Reporter Gene Enzymes其它检测试剂盒2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi1100mFluor™ Dyes and Kits标记试剂盒2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests10058Diagnostic Molecules定量试剂盒2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each38101cAMP GPCR Assays常规细胞株0.00
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔