请使用支持JavaScript的浏览器!
主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Amplite™ Fluorimetric Calcium Quantitation Kit *Red Fluorescence*/36360/200 Tests
产品编号:36360
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Amplite™ Fluorimetric Calcium Quantitation Kit *Red Fluorescence*/36360/200 Tests
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)551/577
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategorySmallMoleculeDetection
MetalIons
RelatedDiagnosticMolecules
BiochemicalAssays
Calciumisessentialforalllivingorganisms,particularlyincellphysiology,wheremovementofthecalciumionCa2+intoandoutofthecytoplasmfunctionsasasignalformanycellularprocesses.Calciumisthefifthmostabundantelementbymassinthehumanbody,whereitisacommoncellularionicmessengerwithmanyfunctions,andservesalsoasastructuralelementinbone.Calciumplaysanimportantroleinmediatingtheconstrictionandrelaxationofbloodvessels,nerveimpulsetransmission,musclecontraction,andhormonesecretion.Theserumlevelofcalciumiscloselyregulatedwithinafairlylimitedrange(9to10.5mg/dL)inthehumanbody.Bothhypocalcaemiaandhypercalcaemiaareseriousmedicaldisorders.Causesoflowcalciumlevelsincludechronickidneyfailure,vitaminDdeficiency,andlowbloodmagnesiumlevelsthatcanoccurinseverealcoholism.Amplite™CalciumDetectionKitusingourproprietaryredfluorescenceprobeprovidesasimplemethodfordetectingcalciuminphysiologysolutions.ThefluorescentsignalcanbeeasilyreadbyfluorescencemicroplatereaderwithEx/Em=540/590nm.TheAmplite™CalciumDetectionKitcanbeperformedinaconvenient96-wellor384-wellmicrotiter-plateformatandeasilyadaptedtoautomationwithnoseparationstepsrequired.Theassaycanbecompletedwithin30minutes.WiththeAmplite™CalciumDetectionKit,wehavedetectedaslittleas0.03mMcalcium.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

Note:Thawallthekitcomponentstoroomtemperaturebeforestartingtheexperiment.


 

1.Preparestocksolutions:

Prepare200XRhodRed™stocksolutionbyadding50µLofsterileH2OintothevialofRhodRed™Indicator(ComponentA).Thestocksolutionshouldbeusedpromptly.Anyremainingsolutionneedstobealiquotedandrefrozenat-20oC.

 

2.Prepareassayreactionmixture:

Prepareassayreactionmixtureaccordingtothefollowingtable,keptfromlight.

 

Table1.Assayreactionmixtureforone96-wellplate

Components

Volume

RhodRed™stocksolution(200X,fromStep1)

25μL

AssayBuffer(ComponentB)

5mL

TotalVolume

5.025mL

 

Table2.Layoutofcalciumstandardsandtestsamplesinasolidblack96-wellmicroplate

BL

BL

TS

TS

….

….

 

 

 

 

 

 

CS1

CS1

….

….

….

….

 

 

 

 

 

 

CS2

CS2

 

 

 

 

 

 

 

 

 

 

CS3

CS3

 

 

 

 

 

 

 

 

 

 

CS4

CS4

 

 

 

 

 

 

 

 

 

 

CS5

CS5

 

 

 

 

 

 

 

 

 

 

CS6

CS6

 

 

 

 

 

 

 

 

 

 

CS7

CS7

 

 

 

 

 

 

 

 

 

 

              Note:CS=CalciumStandards,BL=BlankControl,TS=TestSamples.

 

Table3.Reagentcompositionforeachwell

CalciumStandard

BlankControl

TestSample

Serialdilutions*:50μL

H2O:50μL

50μL

*Note:Addtheseriallydilutedcalciumstandardsfrom3mMto0.003mMintowellsfromCS1toCS7induplicate.

 

3.RunCalciumassay:

3.1   Prepareacalciumstandardbydilutingtheappropriateamountofthe300mMCalciumStandard(ComponentC)intoH2OtoproduceaCalciumconcentrationrangingfrom0to3mM(12mg/dL).A0 mMcalciumcontrolisincludedasblankcontrol.Thefinalcalciumconcentrationswillbetwofoldslower(i.e.,0to1.5mM)withtheadditionofassayreactionmixture(SeeStep3.3).

 

3.2   Add50μLofserialdilutedcalciumstandard(fromStep3.1)intoeachwell.

 

3.3   Add50μLofassayreactionmixture(fromStep2,Table1)toeachwellofcalciumstandard,blankcontrol,andtestsamples(seeStep2,Table3)tomakethetotalcalciumassayvolumeof100µL/well.

Note:Fora384-wellplate,add25μLofsampleand25μLofassayreactionmixtureintoeachwell.

 

3.4   Incubatethereactionfor5to30minutesatroomtemperature,protectedfromlight.

 

3.5   MonitorthefluorescenceintensitywithafluorescenceplatereaderatEx/Em=540/590nm.

References&Citations
CitationExplorer

Highefficiencysingle-stepBiomaterial-basedmicroparticlefabricationviatemplate-directedsupramolecularcoordinationchemistry
Authors:KwokKeiLai,ReinhardRenNEBerg,WingCheungMak
Journal:GreenChemistry(2016):1715--1723

Microcystin-LRcausessexualhormonedisturbanceinmaleratbytargetinggonadotropin-releasinghormoneneurons
Authors:XuetingWang,JieDing,ZouXiang,PeipeiJiang,JingDu,XiaodongHan
Journal:Toxicon(2016):45--55


品牌介绍

AAT Bioquest常用产品报价单

产品名货号公司分类商城分类美金价
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg2iFluor Rapid Tests酸碱缓冲液1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug8B0001Signaling Intermediate Ab重组抗体2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug8A0022Phospho-Specific Ab受体2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug16440Fluorescent Anti-IgGs荧光染料1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug16518Fluorescent Anti-IgGs抗小鼠4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug16900Streptavidin Conjugates其他生物染料1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug8C14708Signaling Intermediate Ab功能性抗体2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate12518Reporter Gene Enzymes其它检测试剂盒2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi1100mFluor™ Dyes and Kits标记试剂盒2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests10058Diagnostic Molecules定量试剂盒2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each38101cAMP GPCR Assays常规细胞株0.00
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔