| Overview |  PrinterFriendlyVersion | 
| Ex/Em(nm) | 650/None | 
| MW | N/A | 
| CAS# | N/A | 
| Solvent | N/A | 
| Storage | F/D/L | 
| Category | SmallMoleculeDetection Anions | 
| Related | ATPases Phosphoproteins BiochemicalAssays | 
1.Prepareassayreagents:
Caution:Phosphate-containingbuffersshouldbeavoidedwhenpreparingthesamples!
1.1 Warmallcomponentsatroomtemperaturebeforeuse.
1.2 Itisstronglyrecommendthatclearmicroplatesorcuvettesbeusedtoachievethebestresults.
2.Prepareseriallydilutedphosphatestandardsand/ortestsamples:
2.1 Add50μLof1mMphosphatestandard(ComponentA)in950μLofdeionizedwaterorenzymereactionbuffertogeta50μMphosphatestandardsolution.
2.2 Take200μLof50μMphosphatestandardsolutiontoperform1:2serialdilutionstoget25,12.5,6.25,3.125,1.56,and0.78μMseriallydilutedphosphatestandards.
2.3 Addphosphate-containingtestsamplesintoaclear96-wellmicroplateasdescribedinTables1and2.
Table1Layoutofphosphatestandardsandtestsamplesinaclear96-wellmicroplate
| BL | BL | TS | TS | …. | …. | 
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| PS1 | PS1 | …. | …. | …. | …. | 
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| PS2 | PS2 | 
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| PS3 | PS3 | 
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| PS4 | PS4 | 
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| PS5 | PS5 | 
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| PS6 | PS6 | 
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| PS7 | PS7 | 
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Note:PS=PhosphateStandards,BL=BlankControl,TS=TestSamples.
Table2.Reagentcompositionforeachwell
| PhosphateStandard | BlankControl | TestSample | 
| SerialDilutions*:80μL | Phosphate-freewaterorbuffer:80μL | 80μL | 
Note:*Addtheserialdilutionsofphosphatestandardfrom0.1μMto50μMintowellsfromPS1toPS7induplicate.
3.RunPhosphoWorks™colorimetricphosphateassay:
3.1 ShakeMGPlus™Reagent(ComponentB)wellbeforeuse.Add20μL/wellofMGPlus™Reagent(ComponentB)intothewellsofphosphatestandards,blankcontrol,andtestsamples.Mixthereagentsthoroughly.
Note:Fora384-wellplate,add40μLofsampleand10μLofMGPlus™Reagent(ComponentB)intoeachwell.
3.2 Ablue-greencolorwilldevelopinthephosphate-containingwellsin10to40minutes.Monitorabsorbanceat600-660nmwithanabsorbancemicroplatereaderoraspectrophotometer.
Note1:Athighphosphateconcentration(>100μM),precipitatesmayform.Diluteyoursamplesandredotheassays.
Note2:Forcuvetteassaythatrequiresthetotalvolumelargerthan100μL,eithermultiplethevolumeofsampleandMGPlus™Reagent(ComponentB)proportionallyordilutethefinalreactionmixturewith1 MH2OS4 or1MHClbeforemeasuringtheabsorption.
| References&Citations |  CitationExplorer | 
VariationofboneformingABIlitywiththephysicochemicalpropertiesofcalciumphosphatebonesubstitutes
Authors:RongquanDuan,DavideBarbieri,XiaomanLuo,JieWeng,ChongyunBao,JoostDeBruijn,HuipinYuan
Journal:BiomaterialsScience(2017)
Influenceofsurfacemicrostructureandchemistryonosteoinductionandosteoclastogenesisbybiphasiccalciumphosphatediscs.
Authors:NLDavison,JSu,HYuan,JJJPvandenBeucken,JDdeBruijn
Journal:(2015)
Submicron-scalesurfacearchitectureoftricalciumphosphatedirectsosteogenesisinvitroandinvivo
Authors:NLDavison,XLuo,TSchoenmaker,VEverts,HYuan,FBarrere-deGroot,JDdeBruijn
Journal:EurCellMater(2014)
Zincincalciumphosphatemediatesboneinduction:invitroandinvivomodel
Authors:XiaomanLuo,DavideBarbieri,NoelDavison,YonggangYan,JoostDdeBruijn,HuipinYuan
Journal:Actabiomaterialia(2014):477--485
AAT Bioquest常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 | 
| AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 | 




