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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Digoxigenin-11-dUTP/17012/25 nmoles
产品编号:17012
市  场 价:¥2900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$145.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Digoxigenin-11-dUTP/17012/25 nmoles
商品介绍
Overview
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Ex/Em(nm)None/None
MW1132.87
CAS#N/A
SolventWater
StorageF/D/L
CategoryNucleicAcidDetection
DNADetection
RelatedPolymerases
Digoxigenin-11-dUTPcanbeenzymaticallyincorporatedintoDNA/CDNAassubstituteforitsnaturalcounterpartdTTP.Theresultingdigoxigenin-labeledDNA/cDNAprobesaresubsequentlydetectedusingdigoxigenin-antibodiesconjugatedwithhorserADIshperoxidase(HRP),alkalinephosphatase(AP)orafluorescentdye.The11-atomlinkerisattachedtotheC5positionofuridinetominimizetheinterferenceofDIGonthesubstrateincorporationbyPCRpolymerase.Typically1:2Digoxigenin-11-dUTP/dTTPratioforPCRandNickTranslation.
References&Citations
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1.   CoxWG,SingerVL.(2004)FluorescentDNAhybridizationprobepreparationusingaminemodificationandreactivedyecoupling.Biotechniques,36,114.

2.   WenJK,ZhangXE,ChengZ,LiuH,ZhouYF,ZhangZP,YangJH,DengJY.(2004)AvisualDNAchipforsimultaneousdetectionofhepatitisBvirus,hepatitisCvirusandhumanimmunodeficiencyvirustype-1.BiosensBioelectron,19,685.

3.   SchoetzauT,LangnerJ,MoyroudE,RoehlI,VonhoffS,KlussmannS.(2003)Aminomodifiednucleobases:functionalizednucleosidetriphosphatesapplicableforSELEX.BioconjugChem,14,919.

4.   WooddellCI,BurgessRR.(2000)TopologyofyeastRNApolymeraseIIsubunitsintranscriptionelongationcomplexesstudiedbyphotoaffinitycross-linking.Biochemistry,39,13405.

5.   NimmakayaluM,HenegariuO,WardDC,Bray-WardP.(2000)Simplemethodforpreparationoffluor/hapten-labeleddUTP.Biotechniques,28,518.

6.   DuplaaC,CouffinhalT,LabatL,MoreauC,LamaziereJM,BonnetJ.(1993)QuantitativeanalysisofpolymerasechainreactionproductsusingbiotinylateddUTPincorporation.AnalBiochem,212,229.

7.   UrabeT,SanoK,TannoM,MizoguchiJ,OtaniM,LeeMH,TakasakiT,KusakabeH,ImagawaDT,NakaiM.(1992)Anon-radioisotopicreversetranscriptaseassayusingbiotin-11-deoxyuridinetriphosphateonprimer-immobilizedmicrotiterplates.JVirolMethods,40,145.

8.   DawsonBA,HermanT,HaasAL,LoughJ.(1991)Affinityisolationofactivemurineerythroleukemiacellchromatin:uniformdistributionofubiquitinatedhistoneH2Abetweenactiveandinactivefractions.JCellBiochem,46,166.

9.   MuhleggerK,HuberE,vonderEltzH,RugerR,KesslerC.(1990)Non-radioactivelabelinganddetectionofnucleicacids.IV.Synthesisandpropertiesofdigoxigenin-modified2"-deoxyuridine-5"-triphosphatesandaphotoactivatableanalogofdigoxigenin(photodigoxigenin).BiolChemHoppeSeyler,371,953.

10.   DawsonBA,HermanT,LoughJ.(1989)AffinityisolationoftranscriptionallyactivemurineerythroleukemiacellDNAusingacleavablebiotinylatednucleotideanalog.JBiolChem,264,12830.


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