请使用支持JavaScript的浏览器!
主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/ADP-ribose-pNP/11700/1 mg
产品编号:11700
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/ADP-ribose-pNP/11700/1 mg
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)405/None
MW724.37
CAS#N/A
SolventWater
StorageF/D/L
CategoryEnzymeDetection
Phosphodiesterases
RelatedBiochemicalAssays
ADP-ribose-pNPisacolorimetricsubstrateforassessingactivityofpoly(ADP-ribose)polymerase(PARP)enzymes.Theabsorbanceofreleasedp-nitrophenolisdeterminedat405nm,andtheslopeofthecalibrationcurveisusedtoconverttheabsorbenciestomolesofproductgenerated.WithADP-ribose-pNPasthecolorimetricsubstrate,PARP-1wasdeterminedtohavethelargestKmandVmaxvalues(151uMand1.30nmolmin-1mg-1respectively)followedbytankyrase-1(82uMand18pmolmin-1mg-1respectively)andVPARP(46uMand2pmolmin-1mg-1respectively).ThiscolorimetricsubstratecanbeusedtodeterminethekineticparametersforPARP-1,tankyrase-1,andVPARP,andtoscreensmall-moleculeinhibitorsofPARP-1,tankyrase-1,andVPARP.ADP-ribose-pNP-basedcontinuousassayhasconsiderableadvantagesoverstandarddiscontinuousPARPassays,enablingthehighthroughputscreeningofPARP-1,tankyrase-1,andVPARPactivitiesandtheirinhibitors.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

ThefollowingrecommendedprocedurecanbeadaptedformeasuringPARP-1,tankyrase-1,andVPARPactivitiesandtheirinhibitors.Theoptimumconditionsmustbedeterminedexperimentallyforeachtest.

1.    Make5-10mMstocksolutioninH2O.Thestocksolutionshouldbeusedpromptly.

Note:Anyunusedsolutionshouldbealiquotedandrefrozenat-20oC.Avoidrepeatedfreeze-thawcyclesandkeepfromlight.

  1. Takeonevialout,andmake0.25mMassaysolutionbydilutingthestocksolutionwithassaybuffer(50mMTris,10mMMgCl2,pH8.0).
  2. Add0.01mL/wellofsamplesolutioninto0.09mL/wellassaysolution(fromStep2)tomakeafinalvolumeof0.1mLina96-wellclearplate.
  3. Monitortheplateusinganabsorbancemicroplatereaderat405nm.
References&Citations
PrinterFriendlyVersion

1.   NottbohmAC,DothagerRS,PuttKS,HoytMT,HergenrotherPJ.(2007)Acolorimetricsubstrateforpoly(ADP-ribose)polymerase-1,VPARP,andtankyrase-1.AngewChemIntEdEngl,46,2066.

2.   MeliE,PangalloM,BarontiR,ChiarugiA,CozziA,Pellegrini-GiampietroDE,MoroniF.(2003)Poly(ADP-ribose)polymeraseasakeyplayerinexcitotoxicityandpost-ischemicbraindamage.ToxicolLett,139,153.

3.   ValdecantosP,BrionesR,MoyaP,GermainA,Huidobro-ToroJP.(2003)PharmacologicalidentificationofP2X1,P2X4andP2X7nucleotidereceptorsinthesmoothmusclesofhumanumbilicalcordandchorionicbloodvessels.Placenta,24,17.

4.   BujalowskiW,JezewskaMJ.(2000)KineticmechanismofnucleotidecofactorbindingtoEscherichiacolireplicativehelicaseDnaBprotein.stopped-flowkineticstudiesusingfluorescent,ribose-,andbase-modifiednucleotideanalogues.Biochemistry,39,2106.

5.   GallettoR,RajendranS,BujalowskiW.(2000)InteractionsofnucleotidecofactorswiththeEscherichiacolireplicationfactorDnaCprotein.Biochemistry,39,12959.

6.   DukeAM,SteeleDS.(1998)EffectsofcaffeineandadeninenucleotidesonCa2+releasebythesarcoplasmicreticuluminsaponin-permeABIlizedfrogskeletalmusclefibres.JPhysiol,513(Pt1),43.

7.   SchroederP,JustI,AktoriesK.(1994)AdeninenucleotidesregulateADP-ribosylationofmembrane-boundactinandactin-bindingtomembranes.EurJCellBiol,63,3.

8.   BarrFA,LeyteA,MollnerS,PfeufferT,ToozeSA,HuttnerWB.(1991)TrimericG-proteinsofthetrans-Golginetworkareinvolvedintheformationofconstitutivesecretoryvesiclesandimmaturesecretorygranules.FEBSLett,294,239.

9.   MignacoJ,ScofanoHM,BarrabinH.(1990)InhibitionandlabelingoftheCa2(+)-ATPasefromsarcoplasmicreticulumbyperiodateoxidizedATP.BiochimBiophysActa,1039,305.

10.   RamanarayananM,SrinivasanPR.(1976)FurtherstudiesontheisolationandpropertiesofpolyriboadenylatepolymerasefromEscherichiacoliPR7(RNaseI-,pnp).JBiolChem,251,6274.


ViewAllAsPDF
品牌介绍

AAT Bioquest常用产品报价单

产品名货号公司分类商城分类美金价
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg2iFluor Rapid Tests酸碱缓冲液1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug8B0001Signaling Intermediate Ab重组抗体2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug8A0022Phospho-Specific Ab受体2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug16440Fluorescent Anti-IgGs荧光染料1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug16518Fluorescent Anti-IgGs抗小鼠4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug16900Streptavidin Conjugates其他生物染料1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug8C14708Signaling Intermediate Ab功能性抗体2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate12518Reporter Gene Enzymes其它检测试剂盒2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi1100mFluor™ Dyes and Kits标记试剂盒2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests10058Diagnostic Molecules定量试剂盒2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each38101cAMP GPCR Assays常规细胞株0.00
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔