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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Amplite™ Fluorimetric Zinc Ion Quantitation Kit/19000/200 Tests
产品编号:19000
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Amplite™ Fluorimetric Zinc Ion Quantitation Kit/19000/200 Tests
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)492/514
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategorySmallMoleculeDetection
MetalIons
RelatedBiochemicalAssays
ZincisanessentialtracemineralelementthatplaysanimportantroleinanumberofBIOLOGicalprocesses.Itisanessentialfactorrequiredformanyenzymes,proteinstructures,andcontrolofgeneticexpression.Zincstatusalsoaffectsbasicprocessesofcelldivision,growth,differentiation,development,andaging.Clinicalsignsofzincdeficiencyincludeacrodermatitis,lowimmunity,diarrhea,poorhealing,stunting,hypogonADIsm,fetalgrowthfailure,teratologyandabortion.Simple,directandautomation-readyproceduresformeasuringarehighlydesirableinresearchanddrugdiscovery.AATBioquest"sAmplite™FluorimetricZincQuantitationKitprovidesasimplemethodfordetectingzincconcentrationinbiologicalsamplesusingourproprietaryMetaltel™Zn520,inwhichZincbindstotheprobewithenhancedfluorescenceatEx/Em=485/525nm.TheZincprobeexhibitsalargeincreaseinfluorescenceinresponsetoZn2+(greaterthan200~300folds).IthashighZn2+-specificitywithlittleresponsestoothermetals,includingCa2+,Mg2+,Mn2+,andCu2+.Theassaycanbeusedwithbiologicalsamplessuchasserum,plasma,andurinewithdetectionsensitivityat0.2µM(13ng/mL).
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Movemouseovergridtodisplaywavelength&intensityvalues.

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Wavelength(nm)


Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      ZnCl2standardsandtestsamplepreparations:

1.1         Add10μLof100mMZnCl2Standardsolution(ComponentC)into990µLAssayBuffer(Component

           B)toget1mMZnCl2standardsolution. 

 

1.2         Add100μLof1mMZincStandardsolution(ComponentC)to900µLAssayBuffer(ComponentB)  

          toget100µMZnCl2Standardsolution.

 

1.3         Take300μLof100µMZnCl2standardsolution(fromStep1.2)toperform1:3serialdilutionstoget

           30,10,3,1,0.3,0.1and0µMseriallydilutedZnCl2standards.

 

1.4         Dilutethetestsampleto5-100µMrangewithAssayBuffer(ComponentB).

 

1.5         Add50μLofZnCl2standards,dilutedsampleandcontrolintoeachwellasshowninTable1and2.


Table1.LayoutofZnCl2Standardsandtestsamplesinasolidblack96-wellmicroplate

BL

BL

TS

TS

….

….

 

 

 

 

 

 

Zn1

Zn1

….

….

….

….

 

 

 

 

 

 

Zn2

Zn2

 

 

 

 

 

 

 

 

 

 

Zn3

Zn3

 

 

 

 

 

 

 

 

 

 

Zn4

Zn4

 

 

 

 

 

 

 

 

 

 

Zn5

Zn5

 

 

 

 

 

 

 

 

 

 

Zn6

Zn6

 

 

 

 

 

 

 

 

 

 

Zn7

Zn7

 

 

 

 

 

 

 

 

 

 

Note:Zn=ZincStandards,BL=BlankControl,TS=TestSamples.

 

Table2.Reagentcompositionforeachwell

ZincStandard

BlankControl

TestSample

Serialdilutions*:50μL

AssayBuffer:50μL

50μL

*Note:AddtheseriallydilutedZnCl2standardsfrom100µMto0.1µMintowellsfromZn1toZn7induplicate.

 

2.      RunZincassay:

2.1          Add25μLofZn520TM(ComponentA)into5mLAssayBuffer(ComponentB)tomakeZnassay

 buffer.Add50μLZnassaybuffertoeachwellofZnCl2standard,blankcontrol,andtestsamples(seeStep1.5,Table1and2)tomakethetotalZnCl2assayvolumeof100µL/well.

Note: Fora384-wellplate,add25μLofsamplesand25μLofassayreactionmixtureintoeachwell.

2.2         Incubatethereactionfor5-10minutesatroomtemperature,protectedfromlight.

2.3         MonitorthefluorescenceincreasewithafluorescenceplatereaderatEx/Em=485/525nm.

References&Citations
CitationExplorer

Afteroxidation,zincnanoparticleslosetheirABIlitytoenhanceresponsestoodorants
Authors:SamanthaHagerty,YasmineDaniels,MelissaSingletary,OlegPustovyy,LudmilaGloba,WilliamAMacCrehan,ShinMuramoto,GheorgheStan,JuneWLau,EdwardEMorrison
Journal:BioMetals(2016):1--14


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