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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Signal Guard™ HRP conjugate stabilizer/11010/50 mL
产品编号:11010
市  场 价:¥27560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1378.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Signal Guard™ HRP conjugate stabilizer/11010/50 mL
商品介绍
Overview
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Ex/Em(nm)None/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryEnzymeDetection
HorserADIshPeroxidase(HRP)
RelatedBiochemicalAssays
ThemajoradvantageofaliquidHRP-conjugateisthatiteliminatesthechanceforhumanerrorwhendissolvingordilutingthelyophilizedorconcentratedconjugate.Addingtoomuchornotenoughbufferresultsinassaytoassayvariation.Untilnow,thedisadvantageofthisformatwastheinherentinstABIlityoftheliquidHRP-conjugates.OurHRP-conjugatestabilizerisformulatedtoallowyoutoprovidepre-diluted,ready-to-useconjugates.Thisformulationcanbeusedtocreatestocksolutions(1:1,000)orready-to-useassayreagents(1:100,000)thatcanbestoredat4°Cfor24monthssoratroomtemperatureforsixmonths.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

Note1:Usebuffers,suchasHepes,Tris,Tricine,Mops,PIPESorMES,thatcontain<10%phosphatewiththisHRPConjugateStabilizer.Bufferscontaining>10%phosphateorsignificantamountsofborateandcarbonatemaycauseprecipitation.

  • DilutetheprimaryorsecondaryperoxidaseconjugatedantibodyorotherproteinorcompoundwiththeHRPConjugateStabilizertothedesired1Xworkingconcentrationdeterminedforyourconjugate.
  • ThisHRPconjugatestabilizerdilutesandstabilizesHRPconjugatesinasinglestep.
  • DonotfreezetheHRPconjugatesoncetheHRPconjugatestabilizerisadded.ManyHRP-conjugatespreparedinthismannermaintainHRPactivityforupto12monthsorlongerwhenproperlystored.
References&Citations
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1.   MiuraT,MuraokaS,FujimotoY.(2003)Inactivationofcreatinekinaseinducedbyquercetinwithhorseradishperoxidaseandhydrogenperoxide.pro-oxidativeandanti-oxidativeactionsofquercetin.FoodChemToxicol,41,759.

2.   SchmidtA,SchumacherJT,ReicheltJ,HechtHJ,BilitewskiU.(2002)MechaNISTicandmolecularinvestigationsonstabilizationofhorseradishperoxidaseC.AnalChem,74,3037.

3.   GuY,ChenY,YeL.(1992)Electronmicroscopicaldemonstrationofhorseradishperoxidasebyuseoftetramethylbenzidineaschromogenandsodiumtungstateasstabilizer(TMB-STmethod):atracingmethodwithhighsensitivityandwellpreservedultrastructuraltissue.JNeurosciMethods,42,1.

4.   CooperAM.(1990)Thehistochemicalreactionofhorseradishperoxidaseinrodent"whole-brain"preparations.ExpBrainRes,82,456.

5.   JoostenEA.(1990)Anultrastructuraldouble-labellingmethod:immunohistochemicallocalizationofcelladhesionmoleculeL1onHRP-labelleddevelopingcorticospinaltractaxonsintherat.Histochemistry,94,645.

6.   DederenPJ,JoostenEA.(1989)Atriple-labelingmethod:HRPanterogradetracttracingcombinedwithdoubleimmunofluorescentcellstainingindevelopingneuraltissueoftherat.JNeurosciMethods,30,71.

7.   LiangFY,WanXC.(1989)ImprovementofthetetramethylbenzidinereactionwithammoniummolyBDateasastabilizerforlightandelectronmicroscopicligand-HRPneurohistochemistry,immunocytochemistryanddouble-labelling.JNeurosciMethods,28,155.

8.   LiangFY,WanXC.(1989)[AmmoniummolybdateasstabilizingagentintetramethylbenzidinereactionofCB-HRPneurohistochemistryanditsapplicationtoelectronmicroscopyandimmunocytochemistry].ZhongguoYiXueKeXueYuanXueBao,11,142.

9.   OluchaF,Martinez-GarciaF,Lopez-GarciaC.(1985)Anewstabilizingagentforthetetramethylbenzidine(TMB)reactionproductinthehistochemicaldetectionofhorseradishperoxidase(HRP).JNeurosciMethods,13,131.

10.   AlbersFJ,KloosterJ,vanderWantJJ.(1984)Potassiumferricyanide:astabilizerforhorseradishperoxidase.JHistochemCytochem,32,1005.


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