AAT Bioquest/Amplite™ IR/11009/1 mg,AAT Bioquest,,AAT Bioquest,aatbio,进口试剂AAT Bioquest直采 AAT Bioquest发光探针,,Array.Array.Array蚂蚁淘厂家直采.

产品名称：AAT Bioquest/Amplite™ IR/11009/1 mg

产品编号：11009

市场价：¥27560.00

场地：美国(厂家直采)

产品分类：生化试剂>染色剂>其他生物染料>

联系QQ：1570468124

电话号码：4000-520-616

邮箱： info@ebiomall.com

美元价：$1378.00

品牌： AAT Bioquest

公司：AAT Bioquest

公司分类：

商品介绍

Overview

PrinterFriendlyVersion

Ex/Em(nm)	647/670
MW	~400
CAS#	N/A
Solvent	DMSO
Storage	F/D/L
Category	EnzymeDetection HorserADIshPeroxidase(HRP)
Related

OurAmplite™IRisafluorogenicperoxidasesubstratethatgeneratesnearinfraredfluorescenceuponreactionwithperoxidaseandH2O2.ItcanbeusedtodetectbothH2O2andperoxidase.Amplite™IRgeneratesasubstancethathasmaximumabsorptionof647nmwithmaximumemissionat670nm.Thisnearinfraredabsorptionandfluorescenceminimizetheassaybackgroundthatisoftencausedbytheautoabsorptionand/orautofluorescenceofBIOLOGicalsamplesthatrarelyabsorblightbeyond600nm.UnlikeotherHRPsubstratessuchasdihydrofluoresceinsanddihydrorhodamines,theair-oxidationofAmplite™IRisminimal.ComparedtoAmplexRed™,Amplite™IRgeneratesthefluorescencethatispH-independentfrompH4to10.Inaddition,ithasexcellentwatersolubility.ItisasuperioralternativetoAmplexRed™forthedetectionsthatrequirelowpHwhereAmplexRed™hassignificantlyreducedfluorescence.WehaveusedAmplite™IRtodetectHRPinquiteafewimmunoassays.Amplite™IRcanalsobeusedtodetecttraceamountofH2O2.BecauseH2O2isproducedinmanyenzymaticredoxreactions,Amplite™IRcanbeusedincoupledenzymaticreactionstodetecttheactivityofmanyoxidasesand/orrelatedenzymes/substratesorcofactorssuchasglucose,acetylcholineandcholesterol,L-glutamate,aminoacidsetc.

Spectrum

AdvancedSpectrumViewer

Movemouseovergridtodisplaywavelength&intensityvalues.

300

400

500

600

700

800

900

Wavelength(nm)

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.PrepareAmplite^TMIRworkingsolution:

1.1 Preparea10to25mMstocksolutionofAmplite^TMIRinhigh-quality,anhydrousDMSO.Thestocksolutionshouldbeusedpromptly.Anyunusedsolutionneedtobealiquotedandrefrozenat<-20^oC.

Note:Avoidrepeatedfreeze-thawcycles,andprotectfromlight.

1.2 Preparea2XAmplite^TMIRworkingsolution:Onthedayoftheexperiment,eitherdissolveAmplite^TMIRsolidinDMSOorthawanaliquotoftheAmplite^TMIRstocksolutionatroomtemperature.Preparea2Xworkingsolutionof100to250µMin50mMphosphatebufferorbufferofyourchoice,pH7with0.8units/mLperoxidase.Amplite^TMIRfinalconcentrationof50to100µMisrecommendedformeasuringH₂O₂concentrationinsolution.

Note:Amplite^TMIRisunstableinthepresenceofthiolssuchasDTTandb-mercaptoethanol.Thiolshigherthan10µM(finalconcentration)couldsignificantlydecreasetheassaydynamicrange.NADHandglutathione(reducedfrom:GSH)mayinterferewiththeassay.

2.RunH₂O₂assayinsupernatants:

2.1 Add50µLof2XAmplite^TMIRworkingsolution(fromStep1.2)intoeachwelloftheH₂O₂standard,blankcontrol,andtestsamplestomakethetotalH₂O₂assayvolumeof100µL/well.

Note:Fora384-wellplate,add25µLofsampleand25µLof2XAmplite^TMIRworkingsolutionintoeachwell.

2.2 Incubatethereactionatroomtemperaturefor0to30minutes,protectedfromlight.

2.3 MonitorthefluorescenceincreaseatEx/Em=640/680nmwithafluorescenceplatereader.

Note:Amplite™IRperoxidasesubstrateiseasytobeself-oxidized,soreadthefluorescenceassoonastheH₂O₂reactionmixtureisaddedtoincreasethesignaltonoiseratio.

2.4 Thefluorescenceinblankwells(withtheassaybufferonly)isusedasacontrol,andissubtractedfromthevaluesforthosewellswiththeH₂O₂reactions.

3.RunH₂O₂assayforcells:

Amplite™IRcanbeusedtomeasurethereleaseofH₂O₂fromcells.Thefollowingisasuggestedprotocolthatcanbemodifiedforyourspecificresearchneeds.

3.1 TheAmplite^TMIRworkingsolutionshouldbepreparedasStep1.2exceptthatthephosphatebuffershouldbereplacedwiththemediathatisusedinthecellculturesystem.Suggestedmediaincluding(a)KrebsRingersPhosphateBuffer(KRPB);(b).HanksBalancedSaltSolution(HBSS);or(c)Serum-freemedia.

3.2 Preparecellsina96-wellplate(50-100µL/well),andactivatethecellsasdesired.

Note:Thenegativecontrols(mediaaloneandnon-activatedcells)areincludedformeasuringbackgroundfluorescence.

3.3 Add50µLofH₂O₂reactionmixture(fromStep1.2)toeachwellofthecells,andthoseofH₂O₂standards.

Note:Fora384-wellplate,add25µLofcellsand25µLofH₂O₂reactionmixtureintoeachwell.

3.4 Incubatethereactionfor0to30minutesatroomtemperature,protectedfromlight.

3.5 MonitorthefluorescenceincreaseatEx/Em=640/680nmwithafluorescenceplatereader.

Note1:Thecontentsoftheplatecanalsobetransferredtoawhiteclearbottomplateandreadbyanabsorbancemicroplatereaderatthewavelengthof670nm.Theabsorptiondetectionhaslowersensitivitycomparedtofluorescencereading.

Note2:Thefluorescencebackgroundincreaseswithtime,thusitisimportanttosubtractthefluorescenceintensityvalueoftheblankwellsforeachdatapoint.

References&Citations

CitationExplorer

AssessmentofTofacitinibandRuxolitinibandtheirAntiInflammatoryEffectsonMyeloperoxidase
Authors:AmberMilton
Journal:(2017)

PatternedPhotonicNitrocelluloseforPseudo-PaperELISA
Authors:JunjieChi,BingbingGao,MiSun,FenglingZhang,EnbenSu,HongLiu,ZhongzeGu
Journal:AnalyticalChemistry(2017)

SpinalCordInflammation:MolecularImagingafterThoracicAorticIschemiaReperfusionInjury
Authors:HassanAlbadawi,JohnWChen,RahmiOklu,YueWu,GregoryWojtkiewicz,BenjaminPulli,JohnDMilner,RichardPCambria,MichaelTWatkins
Journal:Radiology(2016):152222

MyeloperoxidaseNuclearImagingforEpileptogenesis
Authors:YinianZhang,DanielPSeeburg,BenjaminPulli,GregoryRWojtkiewicz,LionelBure,WendyAtkinson,StefanSchob,YoshikoIwamoto,MuhammadAli,WeiZhang
Journal:Radiology(2015):822--830

Myeloperoxidase--Hepatocyte--StellateCellCrossTalkPromotesHepatocyteInjuryandFibrosisinExperimentalNonalcoholicSteatohepatitis
Authors:BenjaminPulli,MuhammadAli,YoshikoIwamoto,MatthiasWGZeller,StefanSchob,JennyJLinnoila,JohnWChen
Journal:Antioxidants&redoxsignaling(2015):1255--1269

Orderedcleavageofmyeloperoxidaseesterbondsreleasesactivesitehemeleadingtoinactivationofmyeloperoxidasebybenzoicacidhydrazideanalogs
Authors:JianshengHuang,ForrestSmith,PeterPanizzi
Journal:Archivesofbiochemistryandbiophysics(2014):74--85

Raisingtheshields:PCRinthepresenceofmetallicsurfacesprotectedbytailor-madecoatings
Authors:FrankDScherag,ThomasBrandstetter,JürgenRühe
Journal:ColloidsandSurfacesB:Biointerfaces(2014):576--582

Measuringmyeloperoxidaseactivityinbiologicalsamples
Authors:BenjaminPulli,MuhammadAli,RezaForghani,StefanSchob,KevinLCHsieh,GregoryWojtkiewicz,JennyJLinnoila,JohnWChen
Journal:PLoSOne(2013):e67976

Micro-volumewall-lessimmunoassaysusingpatternedplanarplates
Authors:KatherineRKozak,JianyongWang,MelvinLye,RashiTakkar,NamyongKim,HyunjaeLee,NooLiJeon,KedanLin,CrystalZhang,WaiLeeTWong
Journal:LabonaChip(2013):1342--1350

Distinguishinginflammationfromtumorandperitumoraledemabymyeloperoxidasemagneticresonanceimaging
Authors:AnneKleijn,JohnWChen,JasonSBuhrman,GregoryRWojtkiewicz,YoshikoIwamoto,MartineLLamfers,AnatOStemmer-Rachamimov,SamuelDRabkin,RalphWeissleder,RobertLMartuza
Journal:ClinicalCancerResearch(2011):4484--4493

品牌介绍

AAT Bioquest常用产品报价单

产品名	货号	公司分类	商城分类	美金价
AAT Bioquest/Fluorescein, disodium salt CAS 518-47-8/2/100 mg	2	iFluor Rapid Tests	酸碱缓冲液	1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug	8B0001	Signaling Intermediate Ab	重组抗体	2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug	8A0022	Phospho-Specific Ab	受体	2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug	16440	Fluorescent Anti-IgGs	荧光染料	1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug	16518	Fluorescent Anti-IgGs	抗小鼠	4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug	16900	Streptavidin Conjugates	其他生物染料	1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug	8C14708	Signaling Intermediate Ab	功能性抗体	2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit Bright Glow/12518/1 plate	12518	Reporter Gene Enzymes	其它检测试剂盒	2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi	1100	mFluor™ Dyes and Kits	标记试剂盒	2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit Blue Color/10058/200 Tests	10058	Diagnostic Molecules	定量试剂盒	2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each	38101	cAMP GPCR Assays	常规细胞株	0.00