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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Amplite™ Fluorimetric Aldehyde Quantitation Kit/10052/200 Tests
产品编号:10052
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Amplite™ Fluorimetric Aldehyde Quantitation Kit/10052/200 Tests
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)360/450
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategorySmallMoleculeDetection
DiagnosticMolecules
RelatedRedoxEnzymes
BiochemicalAssays
Theformation,reactivityandtoxicityofaldehydesoriginatingfromtheperoxidationoflipidsofcellularmembraneshavereceivedgreatattentioninrecentyears.RapidandaccuratemeasurementofaldehydesisanimportanttaskforBIOLOGicalresearch,chemicalresearch,foodindustryandenvironmentalpollutionsurveillance.Thereareafewreagentsorassaykitsavailableforquantifyingthenumberofaldehydes.MostoftheexistingaldehydetestmethodsarebasedonseparationseitherbythetediousandexpensiveHPLC-MSorGC-MS.BothourAmplite™ColorimetricAldehydeQutitationKit(10051)andAmplite™FluorimetricAldehydeQutitationkit(10052)areusedforquantifyingaldehydesathigherpH.Kit10052usesaproprietaryfluorogenicdyethatgeneratesastronglyfluorescentproductuponreactingwithanaldehyde.Kit10052ismuchmoresensitivethanKit10051.Thisfluorimetrickitprovidesasensitivemix-and-readmethodtodetectaslittleas0.1nanomoleofaldehydeina100µLassayvolume(1µM).Theassaycanbeperformedinaconvenient96-wellor384-wellmicrotiter-plateformatandeasilyadaptedtoautomationwithoutaseparationstep.ItssignalcanbeeasilyreadbyafluorescencemicroplatereaderatEx/Em=365/435nm.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Prepare250XAldeLight™Bluestocksolution:

Add40µLofDMSO(ComponentE)intothevialofAldeLight™Blue(ComponentA)tomake250XAldeLight™Bluestocksolution.

Note:TheunusedAldeLight™Bluestocksolutionshouldbedividedintosingleusealiquots,andstoredat-20oC.

2.PrepareAldeLight™Bluereactionmixture:

Add20µLof250XAldeLight™Bluestocksolution(fromStep1)into5mLofAssayBuffer(ComponentB),andmixthemwell.

Note:5mLofAldeLight™Bluereactionmixtureisenoughforoneplate.Thereactionmixtureisnotstable,andbestusedwithin2hours.

3.Prepareserialdilutionsofaldehydestandard(0to1mM):

3.1   Add1mLofAssayBuffer(ComponentB)intothevialofAldehydeStandard(ComponentD)tomakea10mMaldehydestandardstocksolution.

Note:Theunused10mMAldehydestandardstocksolutionshouldbedividedintosingleusealiquotsandstoredat-20oC.

 

3.2   Take100μLof10mMaldehydestandardstocksolution(fromStep3.1)toperform1:10,and1:3serialdilutionstoget1000,300,100,30,10,3,1and0μMserialdilutionsofaldehydestandard.

 

3.3   Addseriallydilutedaldehydestandardsandaldehyde-containingtestsamplesintoasolidblack96-wellmicroplateasdescribedinTables1and2.

Table1.LayoutofAldehydeStandardsandtestsamplesinasolidblack96-wellmicroplate

BL

BL

TS

TS

….

….

 

 

 

 

 

 

AS1

AS1

….

….

….

….

 

 

 

 

 

 

AS2

AS2

 

 

 

 

 

 

 

 

 

 

AS3

AS3

 

 

 

 

 

 

 

 

 

 

AS4

AS4

 

 

 

 

 

 

 

 

 

 

AS5

AS5

 

 

 

 

 

 

 

 

 

 

AS6

AS6

 

 

 

 

 

 

 

 

 

 

AS7

AS7

 

 

 

 

 

 

 

 

 

 

      Note:AS=AldehydeStandards,BL=BlankControl,TS=TestSamples.

 

Table2.Reagentcompositionforeachwell

AldehydeStandard

BlankControl

TestSample

SerialDilutions*:50μL

AssayBuffer:50μL

50μL

*Note:AddtheseriallydilutedAldehydestandardsfrom1μMto1000μM intowellsfromAS1toAS7induplicate.

 

4.Runaldehydeassay:

 

4.1 Add50μLofAldeLight™Bluereactionmixture(fromStep2)intoeachwellofaldehydestandard,blankcontrol,andtestsamples(seeStep3.3)tomakethetotalaldehydeassayvolumeof100µL/well.

Note:Fora384-wellplate,add25μLoftestsampleand25μLofAldeLight™Bluereactionmixtureintoeachwell.

4.2   Incubatethereactionmixtureatroomtemperaturefor15to30minutes,protectedfromlight.

 

4.3   Add25μLofReactionBuffer(ComponentC)intoeachwell(fromStep4.2).

 

4.4   MonitorthefluorescenceincreaseatEx/Em=365/435nmusingafluorescenceplatereader.

References&Citations
CitationExplorer

BiologicalActivityofPeptide-conjugatedPolyionComplexMatricesConsistingofAlginateandChitosan
Authors:ChikaraFujimori,JunKumai,KyotaroNakamura,YingziGu,FumihikoKatagiri,KentaroHozumi,YamatoKikkawa,MotoyoshiNomizu
Journal:PeptideScience(2016)

HepaticDeficiencyofAugmenterofLiverRegenerationExacerbatesAlcohol-InducedLiverInjuryandPromotesFibrosisinMice
Authors:SudhirKumar,JiangWang,RichaRani,ChandrashekharRGandhi
Journal:PloSone(2016):e0147864

Integratedself-assemblingdrugdeliverysystempossessingdualresponsiveandactivetargetingfororthotopicovariancancertheranostics
Authors:Chun-JuiLin,Chen-HsiangKuan,Li-WenWang,Hsi-ChinWu,YunchingChen,Chien-WenChang,Rih-YangHuang,Tzu-WeiWang
Journal:Biomaterials(2016):12--26

Fiber-opticproteasesensorbasedonthedegradationofthingelatinfilms
Authors:BastienSchyrr,StéphanieBoder-Pasche,RéalIscher,RitaSmajda,GuyVoirin
Journal:SensingandBio-SensingResearch(2015):65--73


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