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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Amplite™ Colorimetric Aldehyde Quantitation Kit *Blue Color*/10053/200 Tests
产品编号:10053
市  场 价:¥85560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$4278.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Amplite™ Colorimetric Aldehyde Quantitation Kit *Blue Color*/10053/200 Tests
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)620/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategorySmallMoleculeDetection
DiagnosticMolecules
RelatedRedoxEnzymes
BiochemicalAssays
Theformation,reactivityandtoxicityofaldehydesoriginatingfromtheperoxidationoflipidsofcellularmembraneshavereceivedgreatattentioninrecentyears.RapidandaccuratemeasurementofaldehydesisanimportanttaskforBIOLOGicalresearch,chemicalresearch,foodindustryandenvironmentalpollutionsurveillance.Thereareafewreagentsorassaykitsavailableforquantifyingthenumberofaldehydes.MostoftheexistingaldehydetestmethodsarebasedonseparationseitherbythetediousandexpensiveHPLC-MSorGC-MS.Kit10053usesaproprietarychromogenicdyethatgeneratesastronglycolorproductuponreactingwithanaldehyde.Thiscolorimetrickitprovidesasensitivemix-and-readmethodtodetectaldehydes(0.4uM).Theassaycanbeperformedinaconvenient96-wellor384-wellmicrotiter-plateformatandeasilyadaptedtoautomationwithoutaseparationstep.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

Note:Thawallthekitcomponentstoroomtemperaturebeforestartingtheexperiment.

1.Prepare2XAldeView™Bluereactionmixture:

Add5mLofAssayBuffer(ComponentB)intoonebottleofAldeView™Blue(ComponentA)tomakeAldeView™Bluereactionmixture.

Note:5mLof2XAldeView™Bluereactionmixtureisenoughforoneplate.Thereactionmixtureisnotstable,andbestusedwithin2hours.

 

2.Prepareserialdilutionsofaldehydestandard(0to100µM):

2.1   Add1mLofAssayBuffer(ComponentB)intothevialofAldehydeStandard(ComponentD)tomakea10mMaldehydestandardstocksolution.

Note:Theunused10mMaldehydestandardstocksolutionshouldbedividedintosingleusealiquotsandstoredat

-20oC.

 

2.2   Take100μLof10mMaldehydestandardstocksolution(fromStep2.1)toperform1:100,and1:2serialdilutionstoget100,50,25,12.5,6.25,3.125,1.56and0μMserialdilutionswithAssayBuffer(ComponentB).

 

2.3   Addseriallydilutedaldehydestandardsandaldehyde-containingtestsamplesintoawhite96-wellmicroplatewithclearbottomasdescribedinTables1and2.

Table1.LayoutofAldehydeStandardsandtestsamplesinawhite96-wellmicroplatewithclearbottom

BL

BL

TS

TS

….

….

 

 

 

 

 

 

AS1

AS1

….

….

….

….

 

 

 

 

 

 

AS2

AS2

 

 

 

 

 

 

 

 

 

 

AS3

AS3

 

 

 

 

 

 

 

 

 

 

AS4

AS4

 

 

 

 

 

 

 

 

 

 

AS5

AS5

 

 

 

 

 

 

 

 

 

 

AS6

AS6

 

 

 

 

 

 

 

 

 

 

AS7

AS7

 

 

 

 

 

 

 

 

 

 

 Note:AS=AldehydeStandards,BL=BlankControl,TS=TestSamples.

 

Table2.Reagentcompositionforeachwell

AldehydeStandards

BlankControl

TestSample

Serialdilutions*:50μL

AssayBuffer:50μL

50μL

*Note:Addtheseriallydilutedaldehydestandardsfrom1.56μMto100μMintowellsfromAS1toAS7induplicate.

 

3.Runaldehydeassay:

 

3.1   Add50μLof 2XAldeView™Bluereactionmixture(fromStep1)intoeachwellofaldehydestandard,blankcontrol,andtestsamples(seeStep2.3)tomakethetotalaldehydeassayvolumeof100µL/well.

Note:Fora384-wellplate,add12.5μLoftestsampleand12.5μLof2XAldeView™Bluereactionmixtureintoeachwell.

 

3.2   Incubatethereactionmixtureatroomtemperaturefor20-30minutes(protectedfromlight).

 

3.3   Add50μLofAldeView™BlueEnhancer(ComponentC)intoeachwell.

Note:Fora384-wellplate,add25μLofAldeView™BlueEnhancerintoeachwell.

 

3.4   Monitortheabsorbanceincreaseataround620to660nm(Maxat620nm)usinganabsorbanceplatereader.

References&Citations
CitationExplorer

BiologicalActivityofPeptide-conjugatedPolyionComplexMatricesConsistingofAlginateandChitosan
Authors:ChikaraFujimori,JunKumai,KyotaroNakamura,YingziGu,FumihikoKatagiri,KentaroHozumi,YamatoKikkawa,MotoyoshiNomizu
Journal:PeptideScience(2016)

HepaticDeficiencyofAugmenterofLiverRegenerationExacerbatesAlcohol-InducedLiverInjuryandPromotesFibrosisinMice
Authors:SudhirKumar,JiangWang,RichaRani,ChandrashekharRGandhi
Journal:PloSone(2016):e0147864

Integratedself-assemblingdrugdeliverysystempossessingdualresponsiveandactivetargetingfororthotopicovariancancertheranostics
Authors:Chun-JuiLin,Chen-HsiangKuan,Li-WenWang,Hsi-ChinWu,YunchingChen,Chien-WenChang,Rih-YangHuang,Tzu-WeiWang
Journal:Biomaterials(2016):12--26

Fiber-opticproteasesensorbasedonthedegradationofthingelatinfilms
Authors:BastienSchyrr,StéphanieBoder-Pasche,RéalIscher,RitaSmajda,GuyVoirin
Journal:SensingandBio-SensingResearch(2015):65--73


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