Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | 620/None |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | F/D/L |
Category | SmallMoleculeDetection DiagnosticMolecules |
Related | RedoxEnzymes BiochemicalAssays |
Note:Thawallthekitcomponentstoroomtemperaturebeforestartingtheexperiment.
1.Prepare2XAldeView™Bluereactionmixture:
Add5mLofAssayBuffer(ComponentB)intoonebottleofAldeView™Blue(ComponentA)tomakeAldeView™Bluereactionmixture.
Note:5mLof2XAldeView™Bluereactionmixtureisenoughforoneplate.Thereactionmixtureisnotstable,andbestusedwithin2hours.
2.Prepareserialdilutionsofaldehydestandard(0to100µM):
2.1 Add1mLofAssayBuffer(ComponentB)intothevialofAldehydeStandard(ComponentD)tomakea10mMaldehydestandardstocksolution.
Note:Theunused10mMaldehydestandardstocksolutionshouldbedividedintosingleusealiquotsandstoredat
-20oC.
2.2 Take100μLof10mMaldehydestandardstocksolution(fromStep2.1)toperform1:100,and1:2serialdilutionstoget100,50,25,12.5,6.25,3.125,1.56and0μMserialdilutionswithAssayBuffer(ComponentB).
2.3 Addseriallydilutedaldehydestandardsandaldehyde-containingtestsamplesintoawhite96-wellmicroplatewithclearbottomasdescribedinTables1and2.
Table1.LayoutofAldehydeStandardsandtestsamplesinawhite96-wellmicroplatewithclearbottom
BL | BL | TS | TS | …. | …. |
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AS1 | AS1 | …. | …. | …. | …. |
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AS2 | AS2 |
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AS3 | AS3 |
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AS4 | AS4 |
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AS5 | AS5 |
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AS6 | AS6 |
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AS7 | AS7 |
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Note:AS=AldehydeStandards,BL=BlankControl,TS=TestSamples.
Table2.Reagentcompositionforeachwell
AldehydeStandards | BlankControl | TestSample |
Serialdilutions*:50μL | AssayBuffer:50μL | 50μL |
*Note:Addtheseriallydilutedaldehydestandardsfrom1.56μMto100μMintowellsfromAS1toAS7induplicate.
3.Runaldehydeassay:
3.1 Add50μLof 2XAldeView™Bluereactionmixture(fromStep1)intoeachwellofaldehydestandard,blankcontrol,andtestsamples(seeStep2.3)tomakethetotalaldehydeassayvolumeof100µL/well.
Note:Fora384-wellplate,add12.5μLoftestsampleand12.5μLof2XAldeView™Bluereactionmixtureintoeachwell.
3.2 Incubatethereactionmixtureatroomtemperaturefor20-30minutes(protectedfromlight).
3.3 Add50μLofAldeView™BlueEnhancer(ComponentC)intoeachwell.
Note:Fora384-wellplate,add25μLofAldeView™BlueEnhancerintoeachwell.
3.4 Monitortheabsorbanceincreaseataround620to660nm(Maxat620nm)usinganabsorbanceplatereader.
References&Citations | ![]() CitationExplorer |
BiologicalActivityofPeptide-conjugatedPolyionComplexMatricesConsistingofAlginateandChitosan
Authors:ChikaraFujimori,JunKumai,KyotaroNakamura,YingziGu,FumihikoKatagiri,KentaroHozumi,YamatoKikkawa,MotoyoshiNomizu
Journal:PeptideScience(2016)
HepaticDeficiencyofAugmenterofLiverRegenerationExacerbatesAlcohol-InducedLiverInjuryandPromotesFibrosisinMice
Authors:SudhirKumar,JiangWang,RichaRani,ChandrashekharRGandhi
Journal:PloSone(2016):e0147864
Integratedself-assemblingdrugdeliverysystempossessingdualresponsiveandactivetargetingfororthotopicovariancancertheranostics
Authors:Chun-JuiLin,Chen-HsiangKuan,Li-WenWang,Hsi-ChinWu,YunchingChen,Chien-WenChang,Rih-YangHuang,Tzu-WeiWang
Journal:Biomaterials(2016):12--26
Fiber-opticproteasesensorbasedonthedegradationofthingelatinfilms
Authors:BastienSchyrr,StéphanieBoder-Pasche,RéalIscher,RitaSmajda,GuyVoirin
Journal:SensingandBio-SensingResearch(2015):65--73
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