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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Amplite™ Colorimetric Aldehyde Quantitation Kit/10051/200 Tests
产品编号:10051
市  场 价:¥85560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$4278.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Amplite™ Colorimetric Aldehyde Quantitation Kit/10051/200 Tests
商品介绍
Overview
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Ex/Em(nm)550/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategorySmallMoleculeDetection
DiagnosticMolecules
RelatedRedoxEnzymes
BiochemicalAssays
RapidandaccuratemeasurementofaldehydesisimportanttaskforBIOLOGicalresearch,foodindustry,chemicalresearchandenvironmentalpollutionsurveillance.Therearefewreagentsorassaykitsavailableforquantifyingtheamountofaldehydes.MostofexistingaldehydetestmethodsisbasedonseparationseitherbythetediousandexpensiveHPLC-MSorGC-MS.OurAmplite™ColorimetricAldehydeQuantitationkitusesaproprietarydyethatgeneratesachromogenicproductuponreactingwithanaldehyde.Thekitprovidesasensitive,one-stepcolorimetricmethodtodetectaslittleas1nanoomoleofaldehydeina100µLassayvolume(10µM;Figure1).Theassaycanbeperformedinaconvenient96-wellor384-wellmicrotiter-plateformatandeasilyadaptedtoautomationwithnoseparationstepsrequired.Itssignalcanbeeasilyreadbyabsorbancemicroplatereaderat405or550nm.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Prepare2XAldeView™Yellowreactionmixture:

Add5mLofAssaySolution(ComponentB)intothebottleofAldeView™Yellow(ComponentA),andmixwell.

Note1:5mLofthe2XAldeView™Yellowreactionmixtureisenoughfor1plate.Thereactionmixtureisnotstable.Usewithin2hours.

Note2:Assaysolution(ComponentB)ispotentiallyhazardous.Weargloveswhenhandlingit.

2.Prepareserialdilutionsofaldehydestandard(0to1mM):

 

2.1   Add1mLofDilutionBuffer(ComponentD)intothevialofAldehydeStandard(ComponentC)tomakea10mMaldehydestandardstocksolution.

Note:Theunused10mMAldehydestandardstocksolutionshouldbedividedintosingleusealiquotsandstoredat-20oC.

 

2.2   Take100μLof10mMaldehydestandardstocksolution(fromStep2.1)toperform1:10,and1:3serialdilutionstoget1000,300,100,30,10,3,1,0.3,and0μMserialdilutionsofaldehydestandard.

 

2.3   Addserialdilutionsofaldehydestandardandaldehyde-containingtestsamplesintoa96-wellwhite/clearbottommicroplateasdescribedinTables1and2.

 

Note1:BothBSAandTween20willinterferetheassay,uselessthan0.001%BSAand0.01%Tween20inthesamples.

 

Note2:Ifthealdehyde-containingsamplesarefromtheenzymereactionsuchasfructose-1,6-bisphosphatewithfructose-1,6-bisphosphatealdolase,prepare50µLofenzymereaction(25µLfora384-wellplate)asdesired.Incubatetheenzymereactionat37oCforatleast1hour.Thecomponentsofenzymereactionshouldbeoptimizedasneeded(e.g.anoptimizedbuffersystemmightberequiredforaspecificenzymereaction).

 

Note3:Inmostcases,DilutionBuffer(ComponentD)canalsobeusedforrunningenzymereactionifyoudonothaveanoptimizedenzymebuffer.

 

Table1LayoutofAldehydestandardsandtestsamplesinawhite/clearbottom96-wellmicroplate

BL

BL

TS

TS

….

….

 

 

 

 

 

 

AS1

AS1

….

….

….

….

 

 

 

 

 

 

AS2

AS2

 

 

 

 

 

 

 

 

 

 

AS3

AS3

 

 

 

 

 

 

 

 

 

 

AS4

AS4

 

 

 

 

 

 

 

 

 

 

AS5

AS5

 

 

 

 

 

 

 

 

 

 

AS6

AS6

 

 

 

 

 

 

 

 

 

 

AS7

AS7

 

 

 

 

 

 

 

 

 

 

Note:AS=AldehydeStandards,BL=BlankControl,TS=TestSamples.

 

Table2Reagentcompositionforeachwell

AldehydeStandard

BlankControl

TestSample

SerialDilutions*:50μL

Assaybuffer:50μL

50μL

*Note:Addtheserialdilutionsofaldehydestandardfrom0.3μMto1000μMintowellsfromAS1toAS7induplicate.

 

3.Runaldehydeassay:

 

3.1 Add50μLof2XAldeView™Yellowreactionmixtures(fromStep1)intoeachwellofthealdehydestandard,blankcontrol,andtestsamples(seeStep2.3)tomakethetotalaldehydeassayvolumeof100µL/well.

Note:Fora384-wellplate,add25μLofsampleand25μLofaldehydereactionmixtureintoeachwell.

 

3.2   Incubatethereactionmixtureatroomtemperaturefor30to60minutes,protectedfromlight.

 

3.3   Monitortheabsorbanceincreasewithanabsorbanceplatereaderat405or550nm.

Note:DifferentconcentrationsofthealdehydemightformdifferentcolorswithAldeView™Yellow.Atlowerconcentration,theabsorbanceat405nmgivesthebestresult.However,athigherconcentration,theabsorbancetendstoshiftto550nm.


 

References&Citations
CitationExplorer

BiologicalActivityofPeptide-conjugatedPolyionComplexMatricesConsistingofAlginateandChitosan
Authors:ChikaraFujimori,JunKumai,KyotaroNakamura,YingziGu,FumihikoKatagiri,KentaroHozumi,YamatoKikkawa,MotoyoshiNomizu
Journal:PeptideScience(2016)

HepaticDeficiencyofAugmenterofLiverRegenerationExacerbatesAlcohol-InducedLiverInjuryandPromotesFibrosisinMice
Authors:SudhirKumar,JiangWang,RichaRani,ChandrashekharRGandhi
Journal:PloSone(2016):e0147864

Integratedself-assemblingdrugdeliverysystempossessingdualresponsiveandactivetargetingfororthotopicovariancancertheranostics
Authors:Chun-JuiLin,Chen-HsiangKuan,Li-WenWang,Hsi-ChinWu,YunchingChen,Chien-WenChang,Rih-YangHuang,Tzu-WeiWang
Journal:Biomaterials(2016):12--26

Fiber-opticproteasesensorbasedonthedegradationofthingelatinfilms
Authors:BastienSchyrr,StéphanieBoder-Pasche,RéalIscher,RitaSmajda,GuyVoirin
Journal:SensingandBio-SensingResearch(2015):65--73


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