Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | 490/520 |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | F/D/L |
Category | CellAnalysis CellCytotoxicity |
Related | ApoptosisandCytotoxicity CellApoptosis BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
1. Treatcellswithtestcompoundsforadesiredperiodoftime(4-6hoursforJurkatcellstreatedwithcamptothecin)toinduceapoptosis.
Note:AnnexinVflowcytometricanalysisonadherentcellsisnotroutinelytestedsincespecificmembranedamagemayoccurduringcelldetachmentorharvesting.However,methodsforutilizingAnnexinVforflowcytometryonadherentcelltypeshavebeenpreviouslyreportedbyCasiola-Rosenetal.andvanEngelendetal(seeRefs1and2).
2. Centrifugethecellstoget1-5×105cells/tube.
3. ResUSPendcellsin200μLofAssayBuffer(ComponentB).
4. Add2μLofAnnexinV-iFluor™488(ComponentA)intothecells.
Optional:Add2µLof100XPropidiumIodide(ComponentC)fornecrosiscells.
5. Incubateatroomtemperaturefor30to60minutes,protectedfromlight.
6. Optional:add200to300μLofAssayBuffer(ComponentB)toincreasevolumebeforeanalyzingthecellswithaflowcytometer(SeeStep7).
7. MonitorthefluorescenceintensityofAnnexinV-iFluor™488usingtheFL1channel(Ex/Em=490/525nm),andmeasurethecellviabilitywithpropidiumiodideusingtheFL2channel.
References&Citations | ![]() CitationExplorer |
Insitupolymerizablehydrogelincorporatedwithspecificpathogen-freeporcineplatelet-richplasmaforthereconstructionofthecornealendothelium
Authors:Yung-KaiLin,RuchiSharma,HsuMa,Wen-ShyanChen,Chao-LingYao
Journal:JournaloftheTaiwanInstituteofChemicalEngineers(2017)
NovelregulationsofMEF2-A,MEF2-D,andCACNA1SinthefunctionalincompetenceofADIpose-derivedmesenchymalstemcellsbyinducedindoxylsulfateinchronickidneydisease
Authors:DuyenThiDo,NamNhutPhan,Chih-YangWang,ZhengdaSun,Yen-ChangLin
Journal:Cytotechnology(2016):2589--2604
Shearstress-inducedalterationofepithelialorganizationinhumanrenaltubularcells
Authors:DamienMaggiorani,RomainDissard,MarcyBelloy,Jean-SébastienSaulnier-Blache,AudreyCasemayou,LaureDucasse,SandraGrès,JulieBellière,CécileCaubet,Jean-LoupBascands
Journal:PloSone(2015):e0131416
TargetingaG-protein-coupledreceptoroverexpressedinendocrinetumorsbymagneticnanoparticlestoinducecelldeath
Authors:ClaireSanchez,DarineElHajjDiab,VincentConnord,PascalClerc,EtienneMeunier,BernardPipy,BrunoPayré,ReasmeyPTan,MichelGougeon,JulianCarrey
Journal:AcsNano(2014):1350--1363
AAT Bioquest常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |