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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Zinquin AM *UltraPure grade*/21261/1 mg
产品编号:21261
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Zinquin AM *UltraPure grade*/21261/1 mg
商品介绍
Overview
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Ex/Em(nm)344/385
MW458.48
CAS#N/A
SolventDMSO
StorageF/D/L
CategorySmallMoleculeDetection
MetalIons
RelatedpHandIonIndicators
BiochemicalAssays
ZinquinAMesterisalipophilic,zinc-sensitive,cell-permeablefluorescentprobe.ItisretainedinlivingcellsbecausetheAMesteriscleavedbycytosolicesterasetogiveZinquinthatcarryanegativecharge,preventingitseffluxacrosstheplasmamembrane.Zinquinfluorescentprobesmaybeloadedintocellsbyintheculturemediumcontaining5-40?MZinquinethylesterinPBSwithcalciumandmagnesium(orinculturemedium).CellsarenormallyincubatedwiththeZinquinethylesterfor15-30minutesat37°C.ExactloADIngconcentration,timeandtemperaturedependonthepurposeoftheexperimentandcelltype,andsowillneedtobeoptimizedexperimentally.CellsarewashedinPBSwithculturemediumtoremoveextracellularremainingdye.Cellsareobservedundermicroscopeorusedforconfocalmicroscopy,FACSorspectrofluorimetryanalysis.Zincisthesecondmostabundanttransitionmetalinthebodyanditisessentialascatalytic,structuralandregulatoryion.Zincionsareinvolvedinhomeostasis,immuneresponses,oxidativestress,apoptosisandaging.Zinchasbeenproposedtofunctionasaconventionalneurotransmitterforthepresynapticneuronandasatransmembranesignaltotraversethepostsynapticneuron.AberrantzincmetabolismisassociatedwithmanyneurologicaldiseasesincludingAlzheimer"sdisease,Parkinson"sdiseaseandepilepsy.Themostsuitabletechniqueforinvivomonitoringofzinchasbeenproventobefluorescenceimaging.
References&Citations
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1.   ColvinRA,LaskowskiM,FontaineCP.(2006)Zinquinidentifiessubcellularcompartmentalizationofzincincorticalneurons.Relationtothetraffickingofzincandthemitochondrialcompartment.BrainRes,1085,1.

2.   JansenS,ArningJ,DulcksT,BeyersmannD.(2004)S-NitrosocompoundsinterferewithzincprobingbyZinquin.AnalBiochem,332,145.

3.   HendricksonKM,GeueJP,WynessO,LincolnSF,WardAD.(2003)CoordinationandfluorescenceoftheintracellularZn2+probe[2-methyl-8-(4-toluenesulfonamido)-6-quinolyloxy]aceticacid(ZinquinA)internaryZn2+complexes.JAmChemSoc,125,3889.

4.   SnitsarevV,BuddeT,StrickerTP,CoxJM,KrupaDJ,GengL,KayAR.(2001)FluorescentdetectionofZn(2+)-richvesicleswithZinquin:mechanismofactioninlipidenvironments.BiophysJ,80,1538.

5.   KimberMC,MahadevanIB,LincolnSF,WardAD,TiekinkER.(2000)Thesynthesisandfluorescentpropertiesofanaloguesofthezinc(II)specificfluorophorezinquinester.JOrgChem,65,8204.

6.   ZalewskiPD,JianX,SoonLL,BreedWG,SeamarkRF,LincolnSF,WardAD,SunFZ.(1996)ChangesindistributionoflABIlezincinmousespermatozoaduringmaturationintheepididymisassessedbythefluorophoreZinquin.ReprodFertilDev,8,1097.

7.   ZalewskiPD,ForbesIJ,SeamarkRF,BorlinghausR,BettsWH,LincolnSF,WardAD.(1994)Fluxofintracellularlabilezincduringapoptosis(gene-directedcelldeath)revealedbyaspecificchemicalprobe,Zinquin.ChemBiol,1,153.

8.   CoyleP,ZalewskiPD,PhilcoxJC,ForbesIJ,WardAD,LincolnSF,MahadevanI,RofeAM.(1994)Measurementofzincinhepatocytesbyusingafluorescentprobe,zinquin:relationshiptometallothioneinandintracellularzinc.BiochemJ,303(Pt3),781.


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