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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Protonex™ Green 500 Dextran/21217/1 mg
产品编号:21217
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Protonex™ Green 500 Dextran/21217/1 mg
商品介绍
Overview
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Ex/Em(nm)443/505
MW~110K
CAS#N/A
SolventWater
StorageF/D/L
CategoryCellBIOLOGy
pHandIonIndicators
RelatedCellMetabolism
BiochemicalAssays
Protonex™GreendyedemonstratedpH-dependentfluorescence.UnlikemostoftheexistingfluorescentdyesthataremorefluorescentathigherpH,acidicconditionsenhancethefluorescenceofProtonex™Greendye.ThefluorescenceofProtonex™GreendyeincreasesaspHdecreasesfromneutraltotheacidic.Thelackoffluorescenceoutsidethecelleliminatesthewashsteps.Protonex™Greendyeprovidesapowerfultooltomonitoracidiccellcompartmentssuchasendosomesandlysosomes.Protonex™Greendyeisnon-fluorescentoutsidethecells,butfluorescesbrightlygreeninacidiccompartments(suchasphagosomes,lysosomesandendosomes).ThisProtonex™GreenenablesthespecificdetectionofcellularacidiccompartmentswithreducedsignalvariABIlityandimprovedaccuracyforimagingorflowapplications.Protonex™GreenhasthespectralpropertiessimilartothoseofFITC,makingthecommonfiltersetofFITCreADIlyavailabletotheassaysofProtonex™Green.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Movemouseovergridtodisplaywavelength&intensityvalues.

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Preparecellsasdesired.Forexample,plateadherentcellsovernightingrowthmediumat40,000to80,000cells/well/100µLfor 

      96-wellor10,000to20,000cells/well/25µLfor384-wellplates.

Note: Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensity.

2.PrepareRatioWorks™Protonex™GreenDextranloadingsolution:

2.1   Preparea1mg/mLstocksolutionofProtonex™GreenDextranin1mLofsterilewaterorHanksand20mMHepesbuffer(HHBS).Thestocksolutionshouldbeusedpromptly.Anyunusedsolutionneedtobealiquotedandrefrozenat<-20oC.

Note:Avoidrepeatedfreeze-thawcycles,andprotectfromlight.

2.2   Preparea20-100ug/mLProtonex™GreenDextranloadingsolutioninHHBS.

 

3. RunEndocytosisAssay

3.1   Removethemedium,andadd100µL/well(96-wellplate)or25µL/well(384-wellplate)Protonex™GreenDextranloadingsolutionintothecellplate(fromStep2.2).

Note1: ItisimportanttoreplacethegrowthmediumwithHHBSbuffer(100µL/wellfor96-wellplateor25µL/wellfor384-wellplatebeforedye-loading)ifyourcompoundsinterferewiththeserum.

Note2:RapidtraffickingofProtonex™Greendextranfromearlyendosomestolateendosomesandsubsequentfusionwithlysosomescanoccur.ToaidthevisualizationofProtonex™Greendextranwithintheendosomes,werecommendincreasingthelabelingconcentrationanddecreasingtheloadingtime,andimagingimmediately.

3.2   Incubatethedye-loadingplateatcellincubatorfor5to20minutes.

3.3   Washandreplacethedye-loadingsolutionwithHHBSorgrowthmedium.

3.4   RuntheendocytosisassaybymonitoringthefluorescenceatEx/Em=443/505nm.

References&Citations
CitationExplorer

PHD2isaregulatorforglycolyticreprogramminginmacrophages
Authors:AnnemarieGuentsch,AngelikaBeneke,LijaSwain,KatjaFarhat,ShunmugamNagarajan,BenWielockx,KaaminiRaithatha,JanDudek,PeterRehling,AnkeZieseniss
Journal:MolecularandCellularBiology(2016):MCB--00236


品牌介绍

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