Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | 384/540 |
MW | ~10K |
CAS# | N/A |
Solvent | Water |
Storage | F/D/L |
Category | CellBIOLOGy pHandIonIndicators |
Related | CellMetabolism |
Spectrum | AdvancedSpectrumViewer |
1.Preparecellsasdesired.Forexample,plateadherentcellsovernightingrowthmediumat40,000to80,000cells/well/100µLfor
96-wellor10,000to20,000cells/well/25µLfor384-wellplates.
Note: Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensity.
2.PrepareRatioWorks™PDMPODextranloadingsolution:
2.1 Preparea1mg/mLstocksolutionofRatioWorks™PDMPODextranin1mLofsterilewaterorHanksand20mMHepesbuffer(HHBS).Thestocksolutionshouldbeusedpromptly.Anyunusedsolutionneedtobealiquotedandrefrozenat<-20oC.
Note:Avoidrepeatedfreeze-thawcycles,andprotectfromlight.
2.2 Preparea20-100ug/mLRatioWorks™PDMPODextranloadingsolutioninHHBS.
3. RunEndocytosisAssay
3.1 Removethemedium,andadd100µL/well(96-wellplate)or25µL/well(384-wellplate)RatioWorks™PDMPODextranloadingsolutionintothecellplate(fromStep2.2).
Note1: ItisimportanttoreplacethegrowthmediumwithHHBSbuffer(100µL/wellfor96-wellplateor25µL/wellfor384-wellplatebeforedye-loading)ifyourcompoundsinterferewiththeserum.
Note2:RapidtraffickingofRatioWorks™PDMPOdextranfromearlyendosomestolateendosomesandsubsequentfusionwithlysosomescanoccur.ToaidthevisualizationofRatioWorks™PDMPOdextranwithintheendosomes,werecommendincreasingthelabelingconcentrationanddecreasingtheloadingtime,andimagingimmediately.
3.2 Incubatethedye-loadingplateatcellincubatorfor5to20minutes.
3.3 Washandreplacethedye-loadingsolutionwithHHBSorgrowthmedium.
3.4 RuntheendocytosisassaybymonitoringthefluorescenceatEx=360nm,andEm=450and540nmforratiomeasurements.
References&Citations | ![]() CitationExplorer |
iTRAQ-basedproteomicanalysisofthemetabolismmechanismassociatedwithsiliconresponseinthemarinediatomThalassiosirapseudonana
Authors:ChaoDu,Jun-RongLiang,Dan-DanChen,BinXu,Wen-HaoZhuo,Ya-HuiGao,Chang-PingChen,ChrisBowler,WenZhang
Journal:Journalofproteomeresearch(2014):720--734
AAT Bioquest常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |