Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | 503/528 |
MW | ~600 |
CAS# | N/A |
Solvent | DMSO |
Storage | F/D/L |
Category | CellBIOLOGy pHandIonIndicators |
Related | CellMetabolism BiochemicalAssays |
Spectrum | AdvancedSpectrumViewer |
1.Preparecellsasdesired.Forexample,plateadherentcellsovernightingrowthmediumat40,000to80,000cells/well/100µLfor
96-wellor10,000to20,000cells/well/25µLfor384-wellplates.
Note: Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensity.
2.PrepareRatioWorks™BCFL,AMdye-loADIngsolution:
2.1 Preparea10to20mMstocksolutionofRatioWorks™BCFL,AMinhigh-quality,anhydrousDMSO.Thestocksolutionshouldbeusedpromptly.Anyunusedsolutionneedtobealiquotedandrefrozenat<-20oC.
Note:Avoidrepeatedfreeze-thawcycles,andprotectfromlight.
2.2 Preparea5-50uMRatioWorks™BCFL,AMdye-loadingsolutioninHanksand20mMHepesbuffer(HHBS).
3. RunpHAssay
3.1 Add100µL/well(96-wellplate)or25µL/well(384-wellplate)RatioWorks™BCFL,AMdye-loadingsolutionintothecellplate(fromStep2.3).
Note: ItisimportanttoreplacethegrowthmediumwithHHBSbuffer(100µL/wellfor96-wellplateor25µL/wellfor384-wellplatebeforedye-loading)ifyourcompoundsinterferewiththeserum.
3.2 Incubatethedye-loadingplateatcellincubatorfor30to60minutes.
3.3 Washandreplacethedye-loadingsolutionwithHHBS.
3.4 PreparethecompoundplatesbyusingHHBSoryourdesiredbuffer.
3.5 RunthepHassaybymonitoringthefluorescenceatEx/Em=490/535nm(cutoffat515nm)or505/535nmand430/535nm(cutoffat515nm)forratiomeasurements.Thecompoundadditionis50µL/well(96-wellplate)or25µL/well(384-wellplate).
Note:Theassayshouldbecompletewithin3to5minaftercompoundaddition,howeveraminimumof8mindatacollectionarerecommendedforduringassaydevelopment.
References&Citations | ![]() CitationExplorer |
TheF0F1ATPsynthaseregulateshumanneutrophilmigrationthroughcytoplasmicprotonextrusioncoupledwithATPgeneration
Authors:JunGao,TianZhang,ZhanfangKang,WeijenTing,LingqingXu,DazhongYin
Journal:MolecularImmunology(2017):219--226
OxidativeStress-ActivatedNHE1IsInvolvedinHighGlucose-InducedApoptosisinRenalTubularEpithelialCells
Authors:YiqingWu,MinZhang,RuiLiu,ChunjieZhao
Journal:YonseiMedicalJournal(2016):1252--1259
AAT Bioquest常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |