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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/ReadiUse™ probenecid *25 mM stabilized aqueous solution*/20062/10x10 mL
产品编号:20062
市  场 价:¥42060.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2103.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/ReadiUse™ probenecid *25 mM stabilized aqueous solution*/20062/10x10 mL
商品介绍
Overview
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Ex/Em(nm)None/None
MW307.34
CAS#N/A
SolventN/A
StorageF/D/L
CategoryGPCR
CalciumGPCRAssays
RelatedCellSignaling
BiochemicalAssays
Probenecidisaninhibitoroforganic-aniontransporterslocatedincellmembranes.Thesetransportersoftenextrudefluorescentindicatorsfromcells,andthereforecontributetopoordyeretention.Thisphenomenonusuallycauseshighbackgroundintheassaysthatrequirethegoodretentionofthedyeindicatorsinsidecells.Theuseofprobenecidtoinhibitthetransporteractivity,andthustoreduceleakageoftheintracellulardyeindicatorsisacommonmethodforreducingfluorescencebackgroundofcalciumassays.Thecommonlyusedfreeacidformofprobenecidrequirestheuseof1MNaOHtodissolveitduetoitspoorwatersolubilityinneutralwater.AATBioquestoffertheconvenientReADIuse™water-solubleandheat-stableprobenecidintheformatofapowder,solutionortablet.Theyareconvenienttouseandareaseffectiveasthefreeacidformatthesameconcentration.Eachvialisdilutedwith90mLbuffertomake2.5mMfinalconcentrationofprobenecidforyourassays.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1        Thaw1bottleofCat.#20062atroomtemperaturebeforeuse.

2        Make2.5mMReadiUse™probenecidbuffer:Add1mlofReadiUse™probenecid25mMstABIlizedaqueoussolutionto9mlofHHBS(1XHank’swith20mMHepesbuffer,pH7.0),andmixthemwell.

Note1:10mLof2.5mMReadiuse™probenecidbufferisenoughfor1plate.AliquotandstoretheunusedReadiuse™probenecid25mMstabilizedaqueoussolutionat<-20oC,protectedfromlight.Avoidrepeatedfreeze-thawcycles.

Note2:2.5mMReadiuse™probenecidbuffercontains0.02%Pluronic®F-127,soitisnotnecessarytoaddextraPluronic®F-127inthedye-loadingsolution.

3        Makedye-loadingsolutionforonecellplate:AddDMSOreconstitutedfluorescentcalciumdyes(suchasIndo-1AM,Fura-2AM,Fluo-3AM,Fluo-4AM,Fluo-8AM,Rhod-2AMandRhod-4AM)into2.5mMReadiUse™probenecidbuffer,andmixthemwell.Theworkingsolutionisstableforatleast2hoursatroomtemperature.

References&Citations
CitationExplorer

TeleocidinA2inhibitshumanproteinase-activatedreceptor2signalingintumorcells
Authors:SonjaStahn,LisaThelen,Ina-MariaAlbrecht,JensBitzer,ThomasHenkel,NicoleELISAbethTeusch
Journal:PharmacologyResearch&Perspectives(2016)

Glutathioneadductsonsarcoplasmic/endoplasmicreticulumCa2+ATPaseCys-674regulateendothelialcellcalciumstoresandangiogenicfunctionaswellaspromoteischemicbloodflowrecovery
Authors:MelissaDThompson,YuMei,RobertMWeisbrod,MarcySilver,PraphullaCShukla,VictoriaMBolotina,RichardACohen,XiaoyongTong
Journal:JournalofBIOLOGicalChemistry(2014):19907--19916


品牌介绍

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