Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | None/None |
MW | 307.34 |
CAS# | N/A |
Solvent | N/A |
Storage | F/D/L |
Category | GPCR CalciumGPCRAssays |
Related | CellSignaling BiochemicalAssays |
1 Thaw1bottleofCat.#20062atroomtemperaturebeforeuse.
2 Make2.5mMReadiUse™probenecidbuffer:Add1mlofReadiUse™probenecid25mMstABIlizedaqueoussolutionto9mlofHHBS(1XHank’swith20mMHepesbuffer,pH7.0),andmixthemwell.
Note1:10mLof2.5mMReadiuse™probenecidbufferisenoughfor1plate.AliquotandstoretheunusedReadiuse™probenecid25mMstabilizedaqueoussolutionat<-20oC,protectedfromlight.Avoidrepeatedfreeze-thawcycles.
Note2:2.5mMReadiuse™probenecidbuffercontains0.02%Pluronic®F-127,soitisnotnecessarytoaddextraPluronic®F-127inthedye-loadingsolution.
3 Makedye-loadingsolutionforonecellplate:AddDMSOreconstitutedfluorescentcalciumdyes(suchasIndo-1AM,Fura-2AM,Fluo-3AM,Fluo-4AM,Fluo-8AM,Rhod-2AMandRhod-4AM)into2.5mMReadiUse™probenecidbuffer,andmixthemwell.Theworkingsolutionisstableforatleast2hoursatroomtemperature.
References&Citations | ![]() CitationExplorer |
TeleocidinA2inhibitshumanproteinase-activatedreceptor2signalingintumorcells
Authors:SonjaStahn,LisaThelen,Ina-MariaAlbrecht,JensBitzer,ThomasHenkel,NicoleELISAbethTeusch
Journal:PharmacologyResearch&Perspectives(2016)
Glutathioneadductsonsarcoplasmic/endoplasmicreticulumCa2+ATPaseCys-674regulateendothelialcellcalciumstoresandangiogenicfunctionaswellaspromoteischemicbloodflowrecovery
Authors:MelissaDThompson,YuMei,RobertMWeisbrod,MarcySilver,PraphullaCShukla,VictoriaMBolotina,RichardACohen,XiaoyongTong
Journal:JournalofBIOLOGicalChemistry(2014):19907--19916
AAT Bioquest常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |