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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Pluronic® F-127 *20% solution in DMSO*/20052/10 mL
产品编号:20052
市  场 价:¥21760.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1088.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Pluronic® F-127 *20% solution in DMSO*/20052/10 mL
商品介绍
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Ex/Em(nm)None/None
MW~13kD
CAS#N/A
SolventN/A
StorageRT
CategoryGPCR
CalciumGPCRAssays
RelatedCellSignaling
BiochemicalAssays
Pluronic®F-127isanonionicsurfactantthatis100%activeandrelativelynon-toxictocells,andfrequentlyusedwithdyeAMesterstoimprovetheirwatersolubility.Werecommendthatitbestoredatlowtemperature.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. Mixanequalvolumeof20%Pluronic®F-127DMSOsolutionwiththeAMesterDMSOstocksolution(1-5mM)immediatelybeforeuse.

 

  1.  ThesolutionofAMesterandPluronic®F-127isthendilutedintothecell-loADIngbuffersuchasHanksand20mMHepesbuffer(HHBS)toachieveafinalAMesterconcentrationofbetween1µMand10µM.

 

Note1:Alternatively,onecandilutethe20%Pluronic®F-127stocksolutionintothecell-loadingbufferat1:1000to1:500dilutiontoarchivea0.02to0.04%workingsolutionfirst,andthenaddAMesterDMSOstocksolutiontoachieveafinalAMesterconcentrationofbetween1µMand10µM.

 

Note2:ThefinalconcentrationofPluronic®F-127isnormallykeptatorbelow0.08%.

 

  1. Thecellsareincubatedatadesiredtemperatureforbetween10minutesand1hour.

 

Note:IngeneralitisdesirabletousetheminimumamountofAMesterneededtoachieveadequatefluorescencesignaltonoiselevels.

 

  1. Afterlabeling,thecellsarewashedwithHHBSorfreshmediumbeforestartingtheexperiment.
References&Citations
CitationExplorer

3DprintedlatticesasanactivationandexpansionplatformforTcelltherapy
Authors:BahmanDelalat,FrancesHarding,BatjargalGundsambuu,ElenaMDe-Juan-Pardo,FelixMWunner,Marie-LuiseWille,MarekJasieniak,KristenALMalatesta,HansJGriesser,AntonioSimula
Journal:Biomaterials(2017)

GD2-specificCARTCellsUndergoPotentActivationandDeletionFollowingAntigenEncounterbutcanbeProtectedFromActivation-inducedCellDeathbyPD-1Blockade
Authors:TessaGargett,WenboYu,GianpietroDotti,EricSYvon,SusanNChristo,JohnDHayball,IanDLewis,MalcolmKBrenner,MichaelPBrown
Journal:MolecularTherapy(2016)

InflammasomecomponentsASCandAIM2modulatetheacutephaseofbiomaterialimplant-inducedforeignbodyresponses
Authors:SusanNChristo,KerrilynRDiener,JimManavis,MicheleAGrimbaldeston,AkashBachhuka,KrasimirVasilev,JohnDHayball
Journal:Scientificreports(2016)

Hybridcore/shellmicroparticlesandtheiruseforunderstandingBIOLOGicalprocesses
Authors:AkashBachhuka,SusanNChristo,AlexCavallaro,KerrilynRDiener,AgnieszkaMierczynska,LouiseESmith,RomeoMarian,JimManavis,JohnDHayball,KrasimirVasilev
Journal:Journalofcolloidandinterfacescience(2015):9--17

ScrutinizingcalciumfluxoscillationsinTlymphocytestodeducethestrengthofstimulus
Authors:SusanNChristo,KerrilynRDiener,RobertENordon,MichaelPBrown,HansJGriesser,KrasimirVasilev,FaridCChristo,JohnDHayball
Journal:Scientificreports(2015):7760

IndividualandpopulationquantitativeanalysesofcalciumfluxinT-cellsactivatedonfunctionalizedmaterialsurfaces
Authors:SusanNChristo,GhafarTSarvestani,StefaniSGriesser,BryanRCoad,HansJGriesser,KrasimirVasilev,MichaelPBrown,KerrilynRDiener,JohnDHayball
Journal:AustralianJournalofChemistry(2012):45--49

Solid-statecaptureandreal-timeanalysisofindividualTcellactivationviaself-assemblyofbindingmultimericproteinsonfunctionalizedmaterialssurfaces
Authors:KerrilynRDiener,SusanNChristo,StefaniSGriesser,GhafarTSarvestani,KrasimirVasilev,HansJGriesser,JohnDHayball
Journal:Actabiomaterialia(2012):99--107


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