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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Acridine orange *10 mg/mL solution in water*/17503/10 mL
产品编号:17503
市  场 价:¥21760.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1088.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Acridine orange *10 mg/mL solution in water*/17503/10 mL
商品介绍
Overview
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Ex/Em(nm)500/526
MW301.82
CAS#65-61-2
SolventWater
StorageF/D/L
CategoryNucleicAcidDetection
DNADetection
RelatedLabelingCells
BiochemicalAssays
Acridineorangeisanucleicacidselectivefluorescentcationicdyeusefulforcellcycledetermination.Itiscell-permeable,andinteractswithDNAandRNAbyintercalationorelectrostaticattractions.WhenboundtoDNA,itisverysimilarspectrallytofluorescein,withanexcitationmaximumat502nmandanemissionmaximumat525nm(green).WhenitassociateswithRNA,theexcitationmaximumshiftsto460nm(blue)andtheemissionmaximumshiftsto650nm(red).Thedyeisoftenusedinepifluorescencemicroscopy.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
Sorry,yourbrowserdoesnotsupportinlineSVG.Sorry,yourbrowserdoesnotsupportinlineSVG.
Movemouseovergridtodisplaywavelength&intensityvalues.

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1).Make1-10mMDMSOstocksolution.TheDMSOstocksolutionisgoodfor6monthsifstoredat-20°C.

2).Usethefixationprotocolappropriateforyoursample.

3).PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyestainingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.

4).AddAOstainusingtheconcentrationsbetween1and10µMandincubateitfor15to60minutesasaguide.

Note:Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.

References&Citations
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1.   ShiY,ZhengWW,WuXX,ZhouDH.(2006)[Theinteractionofacridineorangewithanionicsurfactantanditsapplicationtothedeterminationofprotein].GuangPuXueYuGuangPuFenXi,26,1653.

2.   HirumaH,KatakuraT,TakenamiT,IgawaS,KanohM,FujimuraT,KawakamiT.(2006)Vesicledisruption,plasmamembraneblebformation,andacutecelldeathcausedbyIlluminationwithbluelightinacridineorange-loadedmalignantmelanomacells.JPhotochemPhotobiolB.

3.   KrolenkoSA,AdamyanSY,BelyaevaTN,MozhenokTP.(2006)Acridineorangeaccumulationinacidorganellesofnormalandvacuolatedfrogskeletalmusclefibres.CellBiolInt,30,933.

4.   SantiwatanakulS,KriegNR.(2005)AcridineorangestainingandviABIlityofthecoccoidformofCampylobacterupsaliensis.JMedAssocThai,88Suppl1,S5.

5.   CelikA,OgenlerO,ComelekogluU.(2006)Re:Theevaluationofmicronucleusfrequencybyacridineorangefluorescentstaininginperipheralbloodofratstreatedwithleadacetate.(Mutagenesis,20,411-415,2005).Mutagenesis,21,267.

6.   LiuBS,GaoJ,LiuZC,YuLN,YangDC,YangGL.(2006)[Fluorescenceresonanceenergytransferquenchingmethodfordeterminationofarsenicwithacridineorange-rhodamineB].GuangPuXueYuGuangPuFenXi,26,306.

7.   ProudlockRJ.(2006)Re:Theevaluationofmicronucleusfrequencybyacridineorangefluorescentstaininginperipheralbloodofratstreatedwithleadacetate.(Mutagenesis,20,411-415,2005).Mutagenesis,21,265;authorreply267.

8.   ValentiniA,PucciD,CrispiniA,FedericiG,BernardiniS.(2006)AcridineOrangebasedplatinum(II)complexesinducingcytotoxicityandcellcycleperturbationinspiteofGSTP1up-regulation.ChemBiolInteract,161,241.

9.   WangF,YangJ,WuX,WangX,GuoC,JiaZ.(2006)Improvementoftheacridineorange-protein-surfactantsystemforproteinestimationbasedonaromaticringstackingeffectofsodiumdodecylbenzenesulphonate.Luminescence,21,186.

10.   FuseH,AkashiT,MizunoI,NozakiT,WatanabeA.(2006)Postoperativechangesofspermchromatinheterogeneity,usingacridineorangestaining,invaricocelepatients.ArchAndrol,52,223.


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