Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | 335/460 |
MW | 331.28 |
CAS# | N/A |
Solvent | DMSO |
Storage | F/D/L |
Category | NeuroBIOLOGy ReactiveOxygenSpecies |
Related | CellFunctionalAnalysis RedoxEnzymes |
Spectrum | AdvancedSpectrumViewer |
1. PrepareThioliteBlue™AMworkingsolution:
a. Preparea1to5mMstocksolutionofThiolite™Blueinhigh-quality,anhydrousDMSO.Thestocksolutionshouldbeusedpromptly;anyremainingsolutionneedbealiquotedandfrozenat-20°C.
b. Note:TheunusedThioliteBluestocksolutionshouldbedividedintosingleusealiquotsandstoredat-20oC,protectedfromlight.
2. Prepare1Xdyeworkingsolution
a. Preparea1XdyeworkingsolutionbydilutingtheDMSOstocksolution(fromStep1)inHanksand20mMHepesbuffer(HHBS)orthebufferofyourchoice,pH7rightbeforeuse.Mixthemwellbyvortexing.
b. Note:Thefinalconcentrationofthedyeworkingsolutionshouldbeempiricallydeterminedfordifferentcelltypesand/orexperimentalconditions.Itisrecommendedtotestattheconcentrationsthatareatleastoveratenfoldrange.TherecommendconcentrationinJurkatcellsis1-10uM.
3. Analyzecellswithaflowcytometerorafluorescencemicroscope:
a. Treatcellswithtestcompoundsforadesiredperiodoftime.
b. Centrifugethecellstoget1-5×105cellspertube.
c. ResUSPendcellsin1mLofthedyeworkingsolution(fromStep2).Optional:OnecanaddtheDMSOstocksolutionintothecellsdirectlywithoutmediumremoving(suchas,add1µLof1mMDMSOstocksolutioninto1mLcells)
d. Incubatecellswithadyesolutionatroomtemperatureor37°Cfor10to30min,protectedfromlight.
e. Removethedyeworkingsolutionfromthecells,washthecellswithHHBSorbufferofyourchoice.Resuspendcellsin1mLofpre-warmedHHBSormediumtoget2-10×105cellspertube.
f. MonitorthefluorescencechangeatEx/Em335/460nmwithaflowcytometerorafluorescencemicroscope.
References&Citations | ![]() CitationExplorer |
GasChromatography/MassSpectrometry-BasedMetabolomicProfilingRevealsAlterationsinMousePlasmaandLiverinResponsetoFavaBeans
Authors:ManXiao,GuankuiDu,GuobingZhong,DongjingYan,HuazongZeng,WangweiCai
Journal:PloSone(2016):e0151103
Virus-likeparticleswithremovablecyclodextrinsenableglutathione-triggereddrugreleaseincells
Authors:KenichiNiikura,NaotoshiSugimura,YusukeMusashi,ShintaroMikuni,YasutakaMatsuo,ShintaroKobayashi,KeitaNagakawa,ShukoTakahara,ChieTakeuchi,HirofumiSawa
Journal:Molecularbiosystems(2013):501--507
AAT Bioquest常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |