Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | 355/430 |
MW | 461.62 |
CAS# | 115534-33-3 |
Solvent | Water |
Storage | F/D/L |
Category | IonChannels MembranePotentials |
Related | NeurologicalStains BiochemicalAssays |
Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.
1) PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyebindingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.
2) MakeaTMA-DPHDMSOstocksolutionat10-50mM.
3) AddTMA-DPHstainusingtheconcentrationsbetween0.5and5µMinHanksand20mMHepesbufferpH7.4,andincubateitat37°C,5%CO2incubatorfor5minutes.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.
4) WashthecellsandresuspendedintheappropriateHepesbufferpH7.4.
References&Citations | ![]() CitationExplorer |
CytotoxicityandeffluxpumpinhibitioninducedbymolyBDenumdisulfideandboronnitridenanomaterialswithsheet-likestructure
Authors:SuLiu,ZhuoyanShen,BingWu,YueYu,HuiHou,Xu-XiangZhang,Hong-qiangRen
Journal:EnvironmentalScience&Technology(2017)
InteractionofHsp90withphospholipidmodelmembranes
Authors:MuhanZhang,DaoyingWang,PengpengLi,ChongSun,RongXu,ZhimingGeng,WeiminXu,ZhaoqiDai
Journal:BiochimicaetBiophysicaActa(BBA)-Biomembranes(2017)
ComparisonofCytotoxicityandInhibitionofMembraneABCTransportersInducedbyMWCNTswithDifferentLengthandFunctionalGroups
Authors:JingYu,SuLiu,BingWu,ZhuoyanShen,GaryNCherr,Xu-XiangZhang,MeiLi
Journal:Environmentalscience&technology(2016):3985--3994
Lowlevelsofgrapheneandgrapheneoxideinhibitcellularxenobioticdefensesystemmediatedbyeffluxtransporters
Authors:SuLiu,WeiJiang,BingWu,JingYu,HaiyanYu,Xu-XiangZhang,CristinaTorres-Duarte,GaryNCherr
Journal:Nanotoxicology(2016):597--606
AAT Bioquest常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |