Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | None/None |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | R |
Category | MicroBIOLOGy FluorescenceImaging |
Related | BiochemicalAssays |
1 WarmupReadiUse™mammaliancelllysisbuffer*5X*atroomtemperaturebeforeuse.
2 Prepare1XReadiUse™mammaliancelllysisbufferbyadding1mlof5XReadiUse™mammaliancelllysisbufferinto4mlofMili-Qwater,andmixthemwell.
3 The1XReadiUse™mammaliancelllysisbuffer(fromStep2)nowisreadytouse.
4 Treatcellsasdesired,washplatewithPBStoremoveresidualmedia.
5 Thefollowingtableisthesuggestedvolumeforlysisadherentcells.
Plate | 384-well | 96-well | 48-well | 24-well | 12-well | 6-well | 100cm2 |
Volume | 20µL | 50µL | 100µL | 150µL | 200mL | 300mL | 800mL |
6 Lysenon-adherentcellsbyaddingabout100µLof1XReadiUse™mammaliancelllysisbufferto1-5X106cellpellet.
7 Incubateatroomtemperaturefor10-20min.
8 Usethecelllysatedirectlyorcentrifugeitat1500rpmfor5minutes,usethesupernatantfor
9 furtherbiochemicalanalysis.
10 Thecelllysisbuffercanalsobeusedforlysebacterialorplanttissues.Seetheappendixfordetails.
Appendix:TestSamplePreparationsUsing1XCellLysisBuffer
1. PlantCellSamples:
Homogenizeleavewiththelysisbufferat200mg/mL,andcentrifugeat2500rpmfor5-10minutes,usethesupernatantfortests.
2. BacterialCellSamples:
Collectbacterialcellsbycentrifugation((10,000g,0°C,15min).Useabout 100to10millioncells/mLlysisbuffer,keepthetreatedsolutionatroomtemperaturefor15minutes. Centrifugeat2500rpmfor5minutes,andusethesupernatantfortests.
3. TissueSamples:
Weigh~20mgtissue,washwithcoldPBS.Homogenizewith400μloflysisbufferinamicro-centrifugetube.Centrifugeat2500rpmfor5-10minutes,usethesupernatantfortheassay.
References&Citations | ![]() CitationExplorer |
RhinacanthinCAlleviatesAmyloid-βFibrils’ToxicityonNeuronsandAttenuatesNeuroinflammationTriggeredbyLPS,Amyloid-β,andInterferon-γinGlialCells
Authors:Kai-AnChuang,Ming-HanLi,Ni-HsuanLin,Chih-HsuanChang,ILu,IPan,TomoyaTakahashi,Ming-DerPerng,Shu-FangWen
Journal:OxidativeMedicineandCellularLongevity(2017)
ImpactofsiRNAtargetingofβ-cateninondifferentiationofratneuralstemcellsandgeneexpressionofNgn1andBMP4followinginvitrohypoxic-ischemicbraindamage
Authors:XiaoyingZhang,CuicuiZhu,QiongLuo,JvDong,LvLiu,MinLi,HongtaoZhu,XiangpingMa,JunWang
Journal:MolecularMedicineReports(2016):3595--3601
Applicationofinterleukin-22mediatesprotectioninexperimentalacetaminophen-inducedacuteliverinjury
Authors:PatrickScheiermann,MalteBachmann,ItamarGoren,BernhardZwissler,JosefPfeilschifter,HeikoMühl
Journal:TheAmericanjournalofpathology(2013):1107--1113
Synergisticactivityofαvβ3integrinsandtheelastinbindingproteinenhancecell-matrixinteractionsonbioactivehydrogelsurfaces
Authors:DhavalPatel,SusanEVandromme,MichaelEReid,LakeshiaJTaite
Journal:Biomacromolecules(2012):1420--1428
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