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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/ReadiUse™ mammalian cell lysis buffer *5X*/20012/10 mL
产品编号:20012
市  场 价:¥27560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1378.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/ReadiUse™ mammalian cell lysis buffer *5X*/20012/10 mL
商品介绍
Overview
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Ex/Em(nm)None/None
MWN/A
CAS#N/A
SolventN/A
StorageR
CategoryMicroBIOLOGy
FluorescenceImaging
RelatedBiochemicalAssays
AllourReADIUse™reagentsrequireminimalhands-ontime.Thismammaliancelllysisbufferjustrequireasimple5-folddilution.Itiswidelyusedforlyzingcellsinfluorescenceimagingandflowcytometryapplications.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1        WarmupReadiUse™mammaliancelllysisbuffer*5X*atroomtemperaturebeforeuse.

2        Prepare1XReadiUse™mammaliancelllysisbufferbyadding1mlof5XReadiUse™mammaliancelllysisbufferinto4mlofMili-Qwater,andmixthemwell.

3        The1XReadiUse™mammaliancelllysisbuffer(fromStep2)nowisreadytouse.

4        Treatcellsasdesired,washplatewithPBStoremoveresidualmedia.

5        Thefollowingtableisthesuggestedvolumeforlysisadherentcells.

 

Plate

384-well

96-well

48-well

24-well

12-well

6-well

100cm2

Volume

20µL

50µL

100µL

150µL

200mL

300mL

800mL

 

6        Lysenon-adherentcellsbyaddingabout100µLof1XReadiUse™mammaliancelllysisbufferto1-5X106cellpellet.

7        Incubateatroomtemperaturefor10-20min.

8        Usethecelllysatedirectlyorcentrifugeitat1500rpmfor5minutes,usethesupernatantfor

9        furtherbiochemicalanalysis.

10    Thecelllysisbuffercanalsobeusedforlysebacterialorplanttissues.Seetheappendixfordetails.

 

 

 

Appendix:TestSamplePreparationsUsing1XCellLysisBuffer

 

1.            PlantCellSamples:

Homogenizeleavewiththelysisbufferat200mg/mL,andcentrifugeat2500rpmfor5-10minutes,usethesupernatantfortests.

 

2.            BacterialCellSamples:

Collectbacterialcellsbycentrifugation((10,000g,0°C,15min).Useabout 100to10millioncells/mLlysisbuffer,keepthetreatedsolutionatroomtemperaturefor15minutes. Centrifugeat2500rpmfor5minutes,andusethesupernatantfortests.

 

3.            TissueSamples:

Weigh~20mgtissue,washwithcoldPBS.Homogenizewith400μloflysisbufferinamicro-centrifugetube.Centrifugeat2500rpmfor5-10minutes,usethesupernatantfortheassay.

 

References&Citations
CitationExplorer

RhinacanthinCAlleviatesAmyloid-βFibrils’ToxicityonNeuronsandAttenuatesNeuroinflammationTriggeredbyLPS,Amyloid-β,andInterferon-γinGlialCells
Authors:Kai-AnChuang,Ming-HanLi,Ni-HsuanLin,Chih-HsuanChang,ILu,IPan,TomoyaTakahashi,Ming-DerPerng,Shu-FangWen
Journal:OxidativeMedicineandCellularLongevity(2017)

ImpactofsiRNAtargetingofβ-cateninondifferentiationofratneuralstemcellsandgeneexpressionofNgn1andBMP4followinginvitrohypoxic-ischemicbraindamage
Authors:XiaoyingZhang,CuicuiZhu,QiongLuo,JvDong,LvLiu,MinLi,HongtaoZhu,XiangpingMa,JunWang
Journal:MolecularMedicineReports(2016):3595--3601

Applicationofinterleukin-22mediatesprotectioninexperimentalacetaminophen-inducedacuteliverinjury
Authors:PatrickScheiermann,MalteBachmann,ItamarGoren,BernhardZwissler,JosefPfeilschifter,HeikoMühl
Journal:TheAmericanjournalofpathology(2013):1107--1113

Synergisticactivityofαvβ3integrinsandtheelastinbindingproteinenhancecell-matrixinteractionsonbioactivehydrogelsurfaces
Authors:DhavalPatel,SusanEVandromme,MichaelEReid,LakeshiaJTaite
Journal:Biomacromolecules(2012):1420--1428


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