Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | None/None |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | F/D/L |
Category | MicroBIOLOGy FluorescenceImaging |
Related | BiochemicalAssays |
1.Performformaldehydefixation:Incubatethecellswith4%formaldehydefor10-30minutesatroomtemperature.
Note:Avoidanymethanolcontainingfixativessincemethanolcandamageactinduringthefixationprocess.Thepreferredfixativeismethanol-freeformaldehyde.
2.Rinsethefixedcells2-3timesinPBS.
3.Thecellsarereadyforimmunohistochemistry/immunofluorescence.
Note:StorethefixedcellsinPBSat4oC.
References&Citations | ![]() CitationExplorer |
RhinacanthinCAlleviatesAmyloid-βFibrils’ToxicityonNeuronsandAttenuatesNeuroinflammationTriggeredbyLPS,Amyloid-β,andInterferon-γinGlialCells
Authors:Kai-AnChuang,Ming-HanLi,Ni-HsuanLin,Chih-HsuanChang,ILu,IPan,TomoyaTakahashi,Ming-DerPerng,Shu-FangWen
Journal:OxidativeMedicineandCellularLongevity(2017)
ImpactofsiRNAtargetingofβ-cateninondifferentiationofratneuralstemcellsandgeneexpressionofNgn1andBMP4followinginvitrohypoxic-ischemicbraindamage
Authors:XiaoyingZhang,CuicuiZhu,QiongLuo,JvDong,LvLiu,MinLi,HongtaoZhu,XiangpingMa,JunWang
Journal:MolecularMedicineReports(2016):3595--3601
Applicationofinterleukin-22mediatesprotectioninexperimentalacetaminophen-inducedacuteliverinjury
Authors:PatrickScheiermann,MalteBachmann,ItamarGoren,BernhardZwissler,JosefPfeilschifter,HeikoMühl
Journal:TheAmericanjournalofpathology(2013):1107--1113
Synergisticactivityofαvβ3integrinsandtheelastinbindingproteinenhancecell-matrixinteractionsonbioactivehydrogelsurfaces
Authors:DhavalPatel,SusanEVandromme,MichaelEReid,LakeshiaJTaite
Journal:Biomacromolecules(2012):1420--1428
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AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |