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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/FluoroQuest™ Anti-fading Kit I *Optimized for Slide Imaging*/20001/1 kit
产品编号:20001
市  场 价:¥27560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1378.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/FluoroQuest™ Anti-fading Kit I *Optimized for Slide Imaging*/20001/1 kit
商品介绍
Overview
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Ex/Em(nm)None/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryMicroBIOLOGy
FluorescenceImaging
RelatedBiochemicalAssays
Whenexposedtoexcitationlight,fluorescenceintensityofdyesdecreasesduetotheirphotooxidationorotherphotoreactions.Thereareveryfewfluorescentdyesthatcompletelyresistphotobleaching.Frequently,whenasectionhasbeenscannedrepeatedlyunderstrongexcitationlight,dyescouldlosesignificantfluorescencesignalbeforevisualevaluationorphotographycanbeaccomplished.Forexamples,thephotobleachingoffluoresceins(suchasFITC-labeledantibodies)hasbecomeamajorprobleminfluorescencemicroscopy.Inseverecases(suchasphycoprotein-labeledbioconjugates),afluorescenceimageofhighresolutioncannotevenbetakenduetotheextremelyhighphotobleachingrate.Fluoroquest™Anti-FADIngKitistoreducethedyephotobleachingrate,givingresearcherslongerobservationtime.Thekitcontainsalltheessentialcomponentsthatcanbereadilyappliedtoimagingexperiments.Theyareallpremixedandready-to-usesolutions.Thiskitisdesignedforslideformatwhile#20003isdesignedformicroplateformat.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Thawthecomponents:Thawallthekitcomponentsatroomtemperature,andkeepfromlight.

2.Applyanti-fadingreagent:Removeanyexcessliquidfromyourspecimen.Addasmalldropoftheselectedcomponenttothespecimen.Ifthesampleisonaslideortissueculturedish,carefullyplaceacoversliponthedrop,avoidingairbubbles.Ifthesampleisonacoverslip,invertthecoversliponacleanglassslide.Removeanyexcessanti-fadingcomponent.

 

3.Preparesamplesforimaging:Theanti-fadingreagentsshouldbeincubatedfor2hourstoovernight.Forlong-termstorage,sealthecoversliptotheslidewithnailpolishoraplasticsealant.Mountedslidesshouldbestoredat4ºCinthedarkforoptimumsamplelongevity.Thefluorescenceimagingwouldremainstableformanyweeks.Samplescanbeimagedimmediatelyaftermounting.AtypicalimageisshowninFigure1.

 

Note:Theseready-to-useanti-fadingreagentscanbeapplieddirectlyonthewashedspecimen.Althoughthereagentshavebeentestedwithlotsoffixedsamples,theiroptimalanti-fadingefficienciesstronglydependonthepropertiesofyoursamples.Wesuggestthatyoutrymorethanonecomponentforyourimagingsamplestogettheidealcomponent.Forexample,onecomponentmaybemorecompatIBLewithafluorescentlabeledantibodyconjugate(oranenzymesubstrateoraspecialmountingspecimensthatcontainlipophilicplasmamembranestainslikeDiI)thananotherone.

 

References&Citations
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1.   CordesT,VogelsangJ,TinnefeldP.(2009)OnthemechanismofTroloxasantiblinkingandantibleachingreagent.JAmChemSoc,131,5018.

2.   Gallardo-EscarateC,Alvarez-BorregoJ,VonBrandE,DupreE,DelRio-PortillaMA.(2007)RelationshipbetweenDAPI-fluorescencefadingandnuclearDNAcontent:AnalternativemethodtoDNAquantification?BiolRes,40,29.

3.   WidengrenJ,ChmyrovA,EggelingC,LofdahlPA,SeidelCA.(2007)StrategiestoimprovephotostABIlitiesinultrasensitivefluorescencespectroscopy.JPhysChemA,111,429.

4.   DikiciogluE,MeteogluI,OkyayP,CulhaciN,KacarF.(2003)Thereliabilityoflong-termstorageofdirectimmunofluorescentstainingslidesatroomtemperature.JCutanPathol,30,430.

5.   El-RifaiWE,KnuutilaS.(2001)Comparativegenomichybridizationtechnique.MethodsMolMed,50,25.

6.   BerriosM,ConlonKA,ColfleshDE.(1999)Antifadingagentsforconfocalfluorescencemicroscopy.MethodsEnzymol,307,55.

7.   BrockR,HamelersIH,JovinTM.(1999)ComparisonoffixationprotocolsforadherentculturedcellsappliedtoaGFPfusionproteinoftheepidermalgrowthfactorreceptor.Cytometry,35,353.

8.   KolankoCJ,PyleMD,LoatsH,PartonJ,BlakelyWF,NathJ.(1999)Fast-insituhybridizationandimmunoenzymaticcolorpigmentdetectionofmousebonemarrowmicronucleus.BiotechHistochem,74,111.

9.   BenchaibM,DelormeR,PluvinageM,BryonPA,SouchierC.(1996)Evaluationoffivegreenfluorescence-emittingstreptavidin-conjugatedFluorochromesforuseinimmunofluorescencemicroscopy.HistochemCellBiol,106,253.

10.   SwartzDJ,SantiPA.(1996)ImmunofluorescentartifactsduetothepHofantifadingmountingmedia.Biotechniques,20,398.


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