Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | None/None |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | |
Category | MicroBIOLOGy FluorescenceImaging |
Related | BiochemicalAssays |
1.ProteinPrecipitation:
1.1 Prepareaclearsolutionsamplewithproteinconcentrationlessthan50mg/mLafterhomogenizationandcentrifugation.Keepproteinsamplesonice.
Note:Highproteinconcentrationsamplesmightneedtobediluted.
1.2 Add10μLofcoldTCA(ComponentA)into100μLofproteinsampleandvortexbrieflytomixwell.
1.3 Keepthesampleonicefor10minutes.Centrifugeat12,000rpmfor5minutes.Transfersupernatant(deproteinizedsample)toanothertube.
Note:ItisrecommendedtoneutralizesamplesinTCA(Step2.1)anduseforrelevantassaysimmediately.However,deproteinizedsamplesinTCAmaybestoredat-70ºCforuptoonemonth.
2.TCANeutralization:
2.1 ToneutralizeexcessTCA,add10μLofcoldNeutralizationSolution(ComponentB)intothecollectedsupernatantandvortexbrieflytomixwell.
2.2 Keepthesamplesonicefor5minutes.Samplesarenowdeproteinizedandneutralized.
2.3 (Optional)Readabsorbanceat280nm(OD)tocheckifthesamplesaredeproteinized.
References&Citations | ![]() PrinterFriendlyVersion |
1. TuruncE,KaradenizH,ArmaganG,ErdemA,YalcinA.(2013)ElectRochemicaldeterminationofglutathioneinplasmaatcarbonnanotubesbasedscreenprintedelectrodes.CombChemHighThroughputScreen,16,695.
2. ZhangJ,LuY,ChenD.(2012)[Preparationproceduresofanti-complementarypolysaccharidesfromHouttuyniacordata].ZhongguoZhongYaoZaZhi,37,2071.
3. XieLY,ZhangY,PengWH,GanBC.(2011)[StudyonmethodsofdeproteinizationfromPhellinusbaumiipolysaccharide].ZhongYaoCai,34,293.
4. HuangG,ChenY,WangX.(2011)Extractionanddeproteinizationofpumpkinpolysaccharide.IntJFoodSciNutr,62,568.
5. LiuF,ZhuXH,LiuM,ZhouJ,CheXP,HanRF.(2008)[StudyondeproteinizationanddecolorationinextractionofpolysaccharidesfromRaBDosiarubescens].ZhongYaoCai,31,751.
6. HaraT,YamakuraF,TakikawaO,HiramatsuR,KawabeT,IsobeK,NagaseF.(2008)Diazotizationofkynureninebyacidifiednitritesecretedfromindoleamine2,3-dioxygenase-expressingmyeloiddendriticcells.JImmunolMethods,332,162.
7. SamanidouVF,NikolaidouKI,PapadoyannisIN.(2007)DevelopmentandvalidationofanHPLCconfirmatorymethodforthedeterminationofseventetracyclineantibioticsresiduesinmilkaccordingtotheEuropeanUnionDecision2002/657/EC.JSepSci,30,2430.
8. KataokaM,SetoY.(2003)Discriminativedeterminationofalkylmethylphosphonatesandmethylphosphonateinbloodplasmaandurinebygaschromatography-massspectrometryaftertert.-butyldimethylsilylation.JChromatogrBAnalytTechnolBiomedLifeSci,795,123.
9. HemmelderMH,deJongPE,deZeeuwD.(1998)Acomparisonofanalyticproceduresformeasurementoffractionaldextranclearances.JLabClinMed,132,390.
10. KuoTL.(1987)Determinationofparaquatintissueusingion-pairchromatographyinconjunctionwithspectrophotometry.ForensicSciInt,33,177.
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