Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | 495/540 |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | F/D/L |
Category | NucleicAcidDetection DNADetection |
Related | LabelingCells FluorescenceImaging BiochemicalAssays |
1.PreparingGelite™Orangeworkingsolution
1.1 PrepareGelite™Orangeworkingsolutionbyadding1µLofGelite™Orange(ComponentA)into200µLof5XGelLoADIngBuffer(ComponentB). Protecttheworkingsolutionfromlightbycoveringitwithfoilorplacingitinthedark.
2.PrepareDNAloadingsamples
2.1 PrepareDNAsamplesasyoudesired.
2.2 Add4µLofGelite™Orangeworkingsolution(fromStep1.1)into16µLofDNAsamples(fromStep2.1),mixwell.Incubateatroomtemperaturefor5-15minutespriortoelectrophoresis.
2.3 Rungelsbasedonyourstandardprotocol.
2.4 Imagethegelwitha300nmultravioletor254nmtransilluminator,oralaser-basedgelscannerusingalongpathgreenfiltersuchasaSYBR®filterorGelStar®filter.
References&Citations | ![]() PrinterFriendlyVersion |
1. LiuQ,YangZ,HaoH,ChengS,FanW,DuE,XiaoS,WangX,ZhangS.(2014)DevelopmentofaSYBRGreenreal-timeRT-PCRassayforthedetectionofavianencephalomyelitisvirus.JVirolMethods,206C,46.
2. HanakiK,IkeF,KajitaA,YasunoW,YanagibaM,GotoM,SakaiK,AmiY,KyuwaS.(2014)ABroadlyReactiveOne-StepSYBRGreenIReal-TimeRT-PCRAssayforRapidDetectionofMurineNorovirus.PLoSOne,9,e98108.
3. RameshKumarD,SanjukthaM,RajanJJ,AnandaBharathiR,SantiagoTC,AlavandiSV,PoornimaM.(2014)DevelopmentofSYBRGreenbasedrealtimePCRassayfordetectionofmonodonbaculovirusinPenaeusmonodon.JVirolMethods,205C,81.
4. ValiZ,RazA,BokharaeiH,NabaviM,BemanianMH,YazdiMS,DjadidND.(2014)DevelopmentofaHigh-resolutionMeltingAnalysisMethodBasedonSYBRGreen-Iforrs7216389LocusGenotypinginAsthmaticChildPatients.AvicennaJMedBiotechnol,6,72.
5. TajadiniM,PanjehpourM,JavanmardSH.(2014)ComparisonofSYBRGreenandTaqManmethodsinquantitativereal-timepolymerasechainreactionanalysisoffouradenosinereceptorsubtypes.AdvBiomedRes,3,85.
6. KumarJS,SaxenaD,ParidaM.(2014)DevelopmentandcomparativeevaluationofSYBRGreenI-basedone-stepreal-timeRT-PCRassayfordetectionandquantificationofWestNilevirusinhumanpatients.MolCellProbes.
7. GomesTdosS,GarciaMC,deSouzaCunhaF,WerneckdeMacedoH,PeraltaJM,PeraltaRH.(2014)DifferentialdiagnosisofEntamoebaspp.inclinicalstoolsamplesusingSYBRgreenreal-timepolymerasechainreaction.ScientificWorldJournal,2014,645084.
8. LiF,KanB,WangD.(2014)[DevelopmentofbothmultiplePCRandreal-timeSYBRgreenPCRforthedetectionofVibriocholeraenon-O1/O139SEROgroups].ZhonghuaLiuXingBingXueZaZhi,35,66.
9. AnthonyJohnsonAM,DasguptaI,SaiGopalDV.(2014)Developmentofloop-mediatedisothermalamplificationandSYBRgreenreal-timePCRmethodsforthedetectionofCitrusyellowmosaicbadnavirusincitrusspecies.JVirolMethods,203,9.
10. Dubot-PeresA,TanCY,deChesseR,SibounheuangB,VongsouvathM,PhommasoneK,BessaudM,GazinC,ThirionL,PhetsouvanhR,NewtonPN,deLamballerieX.(2014)SYBRgreenreal-timePCRforthedetectionofallenterovirus-A71genogroups.PLoSOne,9,e89963.
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AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |