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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/LDS 751 *CAS 181885-68-7*/17561/25 mg
产品编号:17561
市  场 价:¥27560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1378.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/LDS 751 *CAS 181885-68-7*/17561/25 mg
商品介绍
Overview
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Ex/Em(nm)543/712
MW471.98
CAS#181885-68-7
SolventDMSO
StorageF/D/L
CategoryNucleicAcidDetection
DNADetection
RelatedLabelingCells
FluorescenceImaging
BiochemicalAssays
LDS751isafluorescentcell-permeantnucleicacidstainthatcanbewellexcitedwith488nmlaserlinealthoughithasapeakexcitationat~543nmondsDNA.ItmightbeanalternativetoDRAQ5™,usefulinmulticoloranalysesduetoitslongwavelengthemissionmaximum(~712nm).UponbindingtodsDNAofLDS751has~20-foldfluorescenceenhancement.IthasbeenreportedthatLDS-751isexcludedfromthenucleusandbindsthepolarizedmembranesofmitochondria.ThuscautionsneedbetakenwhenLDS751isusedforanalyzinglivecellsandLDS-751fluorescenceasbeingindicativeofnuclearstatus.Thiazoleorange(anRNAdye)andLDS-751(aDNAdye)compositionhasbeenusedtoseparatereticulocytesfromothercellpopulations.LDS751hasbeenalsousedtoseparateredbloodcellsfromnucleatedcells.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      Makea5-10mMDMSOstocksolution.

 

2.      Add1to10µMintothecells(eithersUSPensionoradherentcells),andstainthecellsfor15to60minutes.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationstodeterminetheoptimalconcentrationthatyieldsthedesiredresult.Highdyeconcentrationtendstocausenonspecificstainingofothercellularstructures.

 

3.      Directlyanalyzethecellularstainingwithafluorescencemicroscope,afluorescencemicroplatereader,orflowcytometer.

References&Citations
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1.   KubotaT,IkedaS,YanagisawaH,YukiM,OkamotoA.(2009)Hybridization-sensitivefluorescentprobeforlong-termmonitoringofintracellularRNA.BioconjugChem,20,1256.

2.   LugoMR,SharomFJ.(2009)InteractionofLDS-751withthedrug-bindingsiteofP-glycoprotein:aTrpfluorescencesteady-stateandlifetimestudy.ArchBiochemBiophys,492,17.

3.   OkamotoA,IkedaS,KubotaT,YukiM,YanagisawaH.(2009)Exciton-controlledfluorescence:applicationtohybridization-sensitivefluorescentDNAprobe.NucleicAcidsSympSer(Oxf),49.

4.   WiczlingP,KrzyzanskiW.(2008)Flowcytometricassessmentofhomeostaticagingofreticulocytesinrats.ExpHematol,36,119.

5.   GuoX,WangS,XiaA,SuH.(2007)Determinationoftheformationofdarkstateviadepletedspontaneousemissioninacomplexsolvatedmolecule.JPhysChemA,111,5800.

6.   MaesML,DavidsonLB,McDonaghPF,RitterLS.(2007)ComparisonofsamplefixationandtheuseofLDS-751oranti-CD45forleukocyteidentificationinmousewholebloodforflowcytometry.JImmunolMethods,319,79.

7.   FritschM,StarrussJ,LoescheA,MuellerS,BleyT.(2005)CellcyclesynchronizationofCupriavidusnecatorbycontinuousphasingmeasuredviaflowcytometry.BiotechnolBioeng,92,635.

8.   LugoMR,SharomFJ.(2005)InteractionofLDS-751andrhodamine123withP-glycoprotein:evidenceforsimultaneousbindingofbothdrugs.Biochemistry,44,14020.

9.   LugoMR,SharomFJ.(2005)InteractionofLDS-751withP-glycoproteinandmappingofthelocationoftheRdrugbindingsite.Biochemistry,44,643.

10.   SetzPD,KnochenmussR.(2005)ExcitonmobilityandtrappinginaMALDImatrix.JPhysChemA,109,4030.


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