请使用支持JavaScript的浏览器!
主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*/17511/100 mg
产品编号:17511
市  场 价:¥3900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$195.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/DAPI [4,6-Diamidino-2-phenylindole, dihydrochloride] *CAS 28718-90-3*/17511/100 mg
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)358/461
MW350.25
CAS#28718-90-3
SolventWater
StorageF/D/L
CategoryNucleicAcidDetection
DNADetection
RelatedLabelingCells
FluorescenceImaging
BiochemicalAssays
DAPIisafluorescentstainthatbindsstronglytoDNA.Itisusedextensivelyinfluorescencemicroscopy.SinceDAPIpassesthroughanintactcellmembrane,itcanbeusedtostainlivecellsbesidesfixedcells.Forfluorescencemicroscopy,DAPIisexcitedwithultravioletlight.Whenboundtodouble-strandedDNAitsabsorptionmaximumisat358nmanditsemissionmaximumisat461nm.OnedrawbackofDAPIisthatitsemissionisfairlybroad.DAPIalsobindstoRNAalthoughitisnotasstronglyfluorescentasitbindstoDNA.Itsemissionshiftstoaround500nmwhenboundtoRNA.DAPI"sblueemissionisconvenientformultiplexingassayssincethereisverylittlefluorescenceoverlapbetweenDAPIandgreen-fluorescentmoleculeslikefluoresceinandgreenfluorescentprotein(GFP),orred-fluorescentstainslikeTexasRed.Besideslabelingcellnuclei,DAPIisalsousedforthedetectionofmycoplasmaorvirusDNAincellcultures.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
Sorry,yourbrowserdoesnotsupportinlineSVG.Sorry,yourbrowserdoesnotsupportinlineSVG.
Movemouseovergridtodisplaywavelength&intensityvalues.

300
400
500
600
700
800
900
Wavelength(nm)


Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

Usethefixationprotocolappropriateforyoursample.DAPIstainingisnormallyperformedafterallotherstaining.

Thefollowingprocedurecanbeadaptedformostcelltypes.Growthmedium,celldensity,thepresenceofothercelltypesandotherfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectrealorapparentstainingofmanyorganisms,causingbrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.

PelletcellsbycentrifugationandresUSPendthecellsinbufferedsaltsolutionsormedia,withoptimaldyebindingatpH7.4.Adherentcellsinculturemaybestainedinsituoncoverslipsorinthecellculturewells.AddDAPIstainusingtheconcentrationsbetween0.5and5µMandincubateitfor15to60minutesasaguide.Ininitialexperiments,itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthatyieldsoptimalstaining.

References&Citations
CitationExplorer

AstrocyticexpressionoftheCXCL12receptor,CXCR7/ACKR3isahallmarkofthediseased,butnotdevelopingCNS
Authors:MaltePuchert,FABIanPelkner,GregorStein,DoychinNAngelov,JohannesBoltze,Daniel-ChristophWagner,FrancescaOdoardi,AlexanderFlügel,WolfgangJStreit,JürgenEngele
Journal:MolecularandCellularNeuroscience(2017)

Cube-shapedtheranosticpaclitaxelprodrugnanocrystalswithsurfacefunctionalizationofSPCandMPEG-DSPEforimagingandchemotherapy
Authors:FuqiangGuo,JiajiaShang,HaiZhao,KangrongLai,YangLi,ZhongxiongFan,ZhenqingHou,GuanghaoSu
Journal:ColloidsandSurfacesB:Biointerfaces(2017)

Legionellapneumophilainfectionactivatesbystandercellsdifferentiallybybacterialandhostcellvesicles
Authors:AnnaLenaJung,ChristinaElenaHerkt,ChristineSchulz,KathrinBolte,KerstinSeidel,NicolettaScheller,AlexandraSittka-Stark,WilhelmBertrams,BerndSchmeck
Journal:ScientificReports(2017)

Peptidesmimickingviralproteinsofporcinecircovirustype2wereprofiledbythespectrumofmouseanti-PCV2antibodies
Authors:Ling-ChuHung,Cheng-YaoYang,Ivan-ChenCheng
Journal:BMCimmunology(2017):25

Poly(ADP-ribose)polymeraseinhibitorsactivatethep53signalingpathwayinneuralstem/Progenitorcells
Authors:AkikoOkuda,SuguruKurokawa,MasanoriTakehashi,AikaMaeda,KatsuyaFukuda,YukariKubo,HyumaNogusa,TomokaTakatani-Nakase,ShujiroOkuda,KunihiroUeda
Journal:BMCneuroscience(2017):14

ApoptoticeffectoftheselectivePPARβ/δagoNISTGW501516ininvasivebladdercancercells
Authors:AdelinePéchery,SylvieFauconnet,HuguesBittard,IsabelleLascombe
Journal:TumorBIOLOGy(2016):1--14

Carbonnanotube--Protaminehybrid:EvaluationofDNAcellpenetration
Authors:CécileCaoduro,RaoudhaKacem,KhaoulaBoukari,FabienPicaud,Claire-HélèneBrachais,DavidMonchaud,ChristopheBorg,HatemBoulahdour,TijaniGharbi,RégisDelage-Mourroux
Journal:Carbon(2016):742--752

IdentificationofepigeneticfactorsregulatingthemesenchymetoepitheliumtransitionbyRNAinterferencescreeninginbreastcancercells
Authors:Jean-MarcGregoire,LaurenceFleury,ClaraSalazar-Cardozo,FrédéricAlby,VéroniqueMasson,PaolaBarbaraArimondo,FrédéricAusseil
Journal:BMCcancer(2016):700

Legionellapneumophila-DerivedOuterMembraneVesiclesPromoteBacterialReplicationinMacrophages
Authors:AnnaLenaJung,CorneliaStoiber,ChristinaEHerkt,ChristineSchulz,WilhelmBertrams,BerndSchmeck
Journal:PLoSPathog(2016):e1005592

Redox-SensitiveCitronellol--CabazitaxelConjugate:MaintainedinVitroCytotoxicityandSelf-AssembledasMultifunctionalNanomedicine
Authors:PengXue,DanLiu,JingWang,NaZhang,JiahuaZhou,LinLi,WeilingGuo,MengchiSun,XiangfeiHan,YongjunWang
Journal:Bioconjugatechemistry(2016):1360--1372


ViewMoreCitations
品牌介绍

AAT Bioquest常用产品报价单

产品名货号公司分类商城分类美金价
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg2iFluor Rapid Tests酸碱缓冲液1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug8B0001Signaling Intermediate Ab重组抗体2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug8A0022Phospho-Specific Ab受体2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug16440Fluorescent Anti-IgGs荧光染料1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug16518Fluorescent Anti-IgGs抗小鼠4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug16900Streptavidin Conjugates其他生物染料1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug8C14708Signaling Intermediate Ab功能性抗体2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate12518Reporter Gene Enzymes其它检测试剂盒2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi1100mFluor™ Dyes and Kits标记试剂盒2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests10058Diagnostic Molecules定量试剂盒2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each38101cAMP GPCR Assays常规细胞株0.00
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔