Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | None/None |
MW | N/A |
CAS# | N/A |
Solvent | N/A |
Storage | F/D/L |
Category | CellBIOLOGy CellMetabolism |
Related | RedoxEnzymes BiochemicalAssays |
Note:OnevialofNADPHisformaking10mLNADPRegeneratingSolution.
Note:AliquotandstoreunusedComponentCat<-20oC,keepfromlightandavoidrepeatedfreeze-thawcycles.
4. Prepare2XNADPRegeneratingSolutionaccordingtothefollowingtables,protectedfromlight.
Table1NADPRegeneratingSolutionforone96-wellplate
Components | Volume |
NADPRegeneratingBufferI | 5mL |
NADPRegeneratingBufferII | 5mL |
NADPH | 0.5mL |
NADPRegeneratingEnzymeMix | 50μL |
Totalvolume | 10.55mL |
Note1:ActivatedNADPregeneratingsolutionisnotstable,andshouldbepreparedfreshlybeforeuse.
Note2:10mLofNADPRegeneratingSolutionisenoughfor1plate.AliquotandstoreunusedComponentA,B,C,Dat<-20oC,keepfromlightandavoidrepeatedfreeze-thawcycles.
5. Addequalvolumeof2XNADPRegeneratingSolution(fromStep4)intothedesiredassaysystem.
References&Citations | ![]() CitationExplorer |
exo-Brevicominbiosynthesisinthefatbodyofthemountainpinebeetle,Dendroctonusponderosae
Authors:MinminSong,AndrewGorzalski,TrangTNguyen,XibeiLiu,ChristopherJeffrey,GaryJBlomquist,ClausTittiger
Journal:Journalofchemicalecology(2014):181--189
exo-BrevicominbiosyntheticpathwayenzymesfromtheMountainPineBeetle,Dendroctonusponderosae
Authors:MinminSong,PatrickDelaplain,TrangTNguyen,XibeiLiu,LeahWickenberg,ChristopherJeffrey,GaryJBlomquist,ClausTittiger
Journal:Insectbiochemistryandmolecularbiology(2014):73--80
FunctionalcharacterizationofmyrcenehydroxylasesfromtwogeographicallydistinctIpspinipopulations
Authors:MinminSong,AmyCKim,AndrewJGorzalski,MarinaMacLean,SharonYoung,MatthewDGinzel,GaryJBlomquist,ClausTittiger
Journal:Insectbiochemistryandmolecularbiology(2013):336--343
Aninsect-specificP450oxidativedecarbonylaseforcuticularhydrocarbonbiosynthesis
Authors:YueQiu,ClausTittiger,ClaudeWicker-Thomas,GaelleLeGoff,SharonYoung,EricWajnberg,ThierryFricaux,NathalieTaquet,GaryJBlomquist,RenéFeyereisen
Journal:ProceedingsoftheNationalAcademyofSciences(2012):14858--14863
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AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |