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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/ReadiUse™ NADP Regenerating Kit/15266/200 Tests
产品编号:15266
市  场 价:¥71060.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$3553.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/ReadiUse™ NADP Regenerating Kit/15266/200 Tests
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)None/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryCellBIOLOGy
CellMetabolism
RelatedRedoxEnzymes
BiochemicalAssays
NADPistheelectronacceptorforbiosyntheticreactionsanddehydrogenationreactions.NADPisusedforanabolicpathways,suchaslipidsynthesis,cholesterolsynthesisandfattyacidchainelongation.Itisalsoanecessarycofactorinmanyxenobioticmetabolismreactions.Inchloroplasts,NADPisreducedtoNADPHbyferredoxin-NADPreductaseinthelaststepoftheelectronchaininphotosynthesisreactions.ManyoxidoreductasesandallligasesuseNADP/NADPHascoenzyme.NADPcanalsobeusedinthedeterminationofamylase,creatinekinase,glucose-6-phosphatedehydrogenase,isocitratedehydrogenase.AATBioquest"sReADIUse™NADPRegeneratingKitprovidesaflexIBLeprepackagedNADPregeneratingsystemtoaccommodatevariousexperimentaldesigns.Itusestwoready-to-usesolutionstoregenerateNADPbyasimplemixing.ThiskitcanbeusedforallNADP-requiringsystems(suchas:12-KetochenodeoxycholicAcidsynthesis,FerredoxinReductaseSystem).300-500enzymeassayscanbeperformedusingthiskit.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. Thawallthecomponentsatroomtemperaturebeforeuse.
  2. Add0.5mLH2OtooneNADPHvial(ComponentD)tomake40XNADPHsolution.

Note:OnevialofNADPHisformaking10mLNADPRegeneratingSolution.

  1. Add100µLH2OtoNADPRegeneratingEnzymeMix(ComponentC)vialtomake400Xstocksolution.

Note:AliquotandstoreunusedComponentCat<-20oC,keepfromlightandavoidrepeatedfreeze-thawcycles.

4.      Prepare2XNADPRegeneratingSolutionaccordingtothefollowingtables,protectedfromlight.

Table1NADPRegeneratingSolutionforone96-wellplate

Components

Volume

NADPRegeneratingBufferI

5mL

NADPRegeneratingBufferII

5mL

NADPH

0.5mL

NADPRegeneratingEnzymeMix

50μL

Totalvolume

10.55mL

Note1:ActivatedNADPregeneratingsolutionisnotstable,andshouldbepreparedfreshlybeforeuse.

Note2:10mLofNADPRegeneratingSolutionisenoughfor1plate.AliquotandstoreunusedComponentA,B,C,Dat<-20oC,keepfromlightandavoidrepeatedfreeze-thawcycles.

5.      Addequalvolumeof2XNADPRegeneratingSolution(fromStep4)intothedesiredassaysystem.

References&Citations
CitationExplorer

exo-Brevicominbiosynthesisinthefatbodyofthemountainpinebeetle,Dendroctonusponderosae
Authors:MinminSong,AndrewGorzalski,TrangTNguyen,XibeiLiu,ChristopherJeffrey,GaryJBlomquist,ClausTittiger
Journal:Journalofchemicalecology(2014):181--189

exo-BrevicominbiosyntheticpathwayenzymesfromtheMountainPineBeetle,Dendroctonusponderosae
Authors:MinminSong,PatrickDelaplain,TrangTNguyen,XibeiLiu,LeahWickenberg,ChristopherJeffrey,GaryJBlomquist,ClausTittiger
Journal:Insectbiochemistryandmolecularbiology(2014):73--80

FunctionalcharacterizationofmyrcenehydroxylasesfromtwogeographicallydistinctIpspinipopulations
Authors:MinminSong,AmyCKim,AndrewJGorzalski,MarinaMacLean,SharonYoung,MatthewDGinzel,GaryJBlomquist,ClausTittiger
Journal:Insectbiochemistryandmolecularbiology(2013):336--343

Aninsect-specificP450oxidativedecarbonylaseforcuticularhydrocarbonbiosynthesis
Authors:YueQiu,ClausTittiger,ClaudeWicker-Thomas,GaelleLeGoff,SharonYoung,EricWajnberg,ThierryFricaux,NathalieTaquet,GaryJBlomquist,RenéFeyereisen
Journal:ProceedingsoftheNationalAcademyofSciences(2012):14858--14863


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