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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/FDGlcU [Fluorescein di-beta-D-glucuronide]/14002/1 mg
产品编号:14002
市  场 价:¥0.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
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品      牌: AAT Bioquest
公      司:AAT Bioquest
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AAT Bioquest/FDGlcU [Fluorescein di-beta-D-glucuronide]/14002/1 mg
商品介绍
Overview
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Ex/Em(nm)490/514
MW684.55
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryEnzymeDetection
HydrolyticEnzymes
RelatedReporterGeneEnzymes
iFluorRapidTests
Thebeta-glucuronidase(GUS)enzymefromE.coli(EC3.2.1.31)hasbeenwelldocumentedtoprovidedesirablecharacteristicsasaMarkergeneintransformedplants.TheGUSreportergenesystemhasmanyadvantagesincludingstableexpressionofE.coliGUSenzyme,nointerferencewithnormalplantmetabolism,andlowintrinsicGUSactivityinhigherplants.FDGlcUisconsideredtobeoneofthemostsensitivefluorogenicsubstratesavailablefordetectingbeta-glucuronidase.ThecolorlessandnonfluorescentFDGlcUishydrolyzedtohighlyfluorescentfluorescein,whichexhibitsexcellentspectralproperties(Ex/Em=492/520nm)thatmatchtheoptimaldetectionwindowofmostfluorescenceinstruments.Glucuronidase-catalyzedhydrolysisofFDGlcUcanbefollowedbyfluorescenceincreasearound520nm.Alternatively,FDGlcUcanalsobeusedtodetectglucuronidaseinachromogenicmodesincetheenzymaticproduct(fluorescein)exhibitsalargeextinctioncoefficient(closeto100,000cm-1mol-1).FDGlcUhasbeenusedforidentifyingGUS-positivecellswithfluorescencemicroscopyandflowcytometry.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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References&Citations
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1.   HsiehJT.(2006)EnhancedtransgeneexpressioninurothelialcancergenetherapywithhistonedeacetylaseinhibitorOkegawaT,NutaharaK,PongRC,HigashiharaE,HsiehJT.DepartmentofUrology,UniversityofKyorin,Tokyo,Japan.UrolOncol,24,565.

2.   MorethK,RiesterD,HildmannC,HempelR,WegenerD,SchoberA,SchwienhorstA.(2006)Activesitetyrosineisessentialforamidohydrolasebutnotforesteraseactivityofaclass2histonedeacetylase-likebacterialenzyme.BiochemJ.

3.   BelienA,DeSchepperS,FlorenW,JanssensB,MarienA,KingP,VanDunJ,AndriesL,VoetenJ,BijnensL,JanicotM,ArtsJ.(2006)Real-timegeneexpressionanalysisinhumanxenograftsforevaluationofhistonedeacetylaseinhibitors.MolCancerTher,5,2317.

4.   Takahashi-FujigasakiJ,FujigasakiH.(2006)Histonedeacetylase(HDAC)4involvementinbothLewyandMarinescobodies.NeuropatholApplNeurobiol,32,562.

5.   OlaharskiAJ,JiZ,WooJY,LimS,HubbardAE,ZhangL,SmithMT.(2006)Thehistonedeacetylaseinhibitortrichostatinahasgenotoxiceffectsinhumanlymphoblastsinvitro.ToxicolSci,93,341.

6.   EyupogluIY,HahnenE,TrankleC,SavaskanNE,SiebzehnrublFA,BusleiR,LemkeD,WickW,FahlbuschR,BlumckeI.(2006)ExperimentaltherapyofmalignantgliomasusingtheinhibitorofhistonedeacetylaseMS-275.MolCancerTher,5,1248.

7.   FongPM,TianL,ChenZJ.(2006)ArABIdopsisthalianahistonedeacetylase1(AtHD1)islocalizedineuchromaticregionsanddemonstrateshistonedeacetylaseactivityinvitro.CellRes,16,479.

8.   OakesML,SiddiqiI,FrenchSL,VuL,SatoM,ArisJP,BeyerAL,NomuraM.(2006)RoleofhistonedeacetylaseRpd3inregulatingrRNAgenetranscriptionandnucleolarstructureinyeast.MolCellBiol,26,3889.

9.   WooHJ,LeeSJ,ChoiBT,ParkYM,ChoiYH.(2006)InductionofapoptosisandinhibitionoftelomeraseactivitybytrichostatinA,ahistonedeacetylaseinhibitor,inhumanleukemicU937cells.ExpMolPathol.

10.   HsiaoCH,LiW,LouTF,BaligaBS,PaceBS.(2006)Fetalhemoglobininductionbyhistonedeacetylaseinhibitorsinvolvesgenerationofreactiveoxygenspecies.ExpHematol,34,264.


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