请使用支持JavaScript的浏览器!
主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/DCFH-DA [2',7'-Dichlorodihydrofluorescein diacetate] *CAS 4091-99-0*/15204/25 mg
产品编号:15204
市  场 价:¥21760.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1088.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/DCFH-DA [2',7'-Dichlorodihydrofluorescein diacetate] *CAS 4091-99-0*/15204/25 mg
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)504/529
MW487.29
CAS#4091-99-0
SolventDMSO
StorageF/D/L
CategoryEnzymeDetection
RedoxEnzymes
RelatedCellSignalingMolecules
ReactiveOxygenSpecies
BiochemicalAssays
DCFH-DA,2",7"-Dichlorodihydrofluoresceindiacetate(alsocalled2",7"-dichlorofluorescindiacetate),ishydrolyzedbycellularesterasesto2",7"-dichlorodihydrofluorescein(alsocalled2",7"-dichlorofluorescin)andisthenoxidizedto2",7"-dichlorofluoresceinprimarilybyH2O2.2",7"-dichlorodihydrofluoresceindiacetatemightbereactivetowardabroadrangeofoxidizingreactionsthatmaybeincreasedduringintracellularoxidantstress.Thisprobeiswidelyusedtomonitoringcellularredoxprocesses.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
Sorry,yourbrowserdoesnotsupportinlineSVG.Sorry,yourbrowserdoesnotsupportinlineSVG.
Movemouseovergridtodisplaywavelength&intensityvalues.

300
400
500
600
700
800
900
Wavelength(nm)


Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. Make1-10mMDMSOstocksolution.TheunusedDMSOstocksolutionshouldbealiquotedintoasingleusevialandstoredat-20°C.Keepfromlight.
  2. Makethedyeworkingconcentrationof1-10µMinaphysiologicalbuffer(suchasPBS,HBSS,HEPES).Theoptimalworkingconcentrationforyourapplicationmustbeempiricallydetermined.
  3. Removecellsfromgrowthmedia,addthedyeworkingsolution(fromStep2)tothecells,andincubatethecellsatRTor37oCfor5to60minutes.
  4. Removethedyeworkingsolution;washwithpre-warmedHBSS,andaddpre-warmedHBSSorgrowthmediumandincubateattheoptimaltemperature.Theoptimalrecoverytimecanvarywidely,assomecelltypesnormallyexhibitlowlevelsofesteraseactivity.
  5. Determinethebaselinefluorescenceintensityofasampleoftheloadedcellspriortoexposingthecellstoexperimentalinducements.
  6. Negativecontrolsshouldbeassessedasfollows:

6.1Examineunstainedcellsforautofluorescenceinthegreenemissionrange.

6.2Forflowcytometry,ascertainthattheforwardandsidescatterofcellsisunchangedafterdye-loADIngandtreatment.Changesincelldimensionsmayberelatedtoblebbingorshrinkageresultingfromhandlingoratoxicresponse.

6.3Examinethefluorescenceofcell-freemixturesofdyeandbuffer/mediawithandwithouttheinducer.Intheabsenceofextracellularesterasesandotheroxidativeenzymes,thegradualincreaseinfluorescenceovertimemayberelatedtospontaneoushydrolysis,atmosphericoxidation,and/orlight-inducedoxidation.

6.4Examinethefluorescenceofuntreated(control)loadedcellsthathavebeenmaintainedingrowthmediumorsimplebuffer.Inhealthycells,oxygenradicalsareeliminatedbycellularenzymesand/ornaturalantioxidants.Followingthedye-loadingrecoveryperiod,healthycellsshouldexhibitalowleveloffluorescencethatisrelativelystableforthedurationoftheexperiment;however,agradualincrease(duetoauto-oxidation)ordecrease(duetolossofdyefromcellsorphotobleaching)influorescencemaybeobserved.Intheabsenceofanystimulusorinducement,aburstoffluorescenceinhealthy,untreatedcellscouldindicateprogresstocelldeathorsomeotheroxidativeevent.

  1. PositivecontrolsmaybestimulatedwithH2O2ortert-butylhydroperoxide(TBHP)toafinalconcentrationof~100µM(increaseordecreasedosebasedonthesensitivityandresponseofthecells).
References&Citations
PrinterFriendlyVersion

1.   DiazG,LiuS,IsolaR,DianaA,FalchiAM.(2003)Mitochondriallocalizationofreactiveoxygenspeciesbydihydrofluoresceinprobes.HistochemCellBiol,120,319.

2.   SaengkhaeC,LoetchutinatC,Garnier-SuillerotA.(2003)Kineticanalysisoffluoresceinanddihydrofluoresceineffluxesintumourcellsexpressingthemultidrugresistanceprotein,MRP1.BiochemPharmacol,65,969.

3.   HempelSL,BuettnerGR,O"MalleyYQ,WesselsDA,FlahertyDM.(1999)Dihydrofluoresceindiacetateissuperiorfordetectingintracellularoxidants:comparisonwith2",7"-dichlorodihydrofluoresceindiacetate,5(and6)-carboxy-2",7"-dichlorodihydrofluoresceindiacetate,anddihydrorhodamine123.FreeRadicBiolMed,27,146.

4.   SchillingE,DopkeHH.(1978)ArapiddiagnostictestfortheviABIlityofearlycattleandrabbitembryosusingdiacetyl-fluorescin.Naturwissenschaften,65,658.

5.   RobertsonEJ,PatelJA.(1968)Determinationoffluorescinsodiuminophthalmicsolutions.AmJHospPharm,25,598.

6.   ManciniAM,ZampaGA,CostanziG.(1961)[Experimentalresearchoninsulinantibodies.II.Immunochemicalcharacterizationofguineapiganti-oxinsulinserumconjugatedwithfluorescinisothiocyanate.].BollSocItalBiolSper,37,1230.

7.   CostanziG,ManciniUM,ZampaGA.(1961)[Experimentalresearchoninsulinantibodies.III.Immunohistologyoftheendocrinepancreaswithguineapiganti-bovineinsulinserum,conjugatedwithfluorescinisothiocyanate.].BollSocItalBiolSper,37,1233.

8.   KingEO,WardMK,RaneyDE.(1954)Twosimplemediaforthedemonstrationofpyocyaninandfluorescin.JLabClinMed,44,301.

9.   TotterJR,MoseleyFT.(1953)InfluenceoftheconcentrationofironontheproductionoffluorescinbyPseudomonasaeruginosa.JBacteriol,65,45.

10.   HurstL,LowburyEJ.(1952)TransferoffluorescinfromPs.pyocyaneatocoloniesofotherbacteria.JClinPathol,5,359.


ViewAllAsPDF
品牌介绍

AAT Bioquest常用产品报价单

产品名货号公司分类商城分类美金价
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg2iFluor Rapid Tests酸碱缓冲液1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug8B0001Signaling Intermediate Ab重组抗体2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug8A0022Phospho-Specific Ab受体2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug16440Fluorescent Anti-IgGs荧光染料1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug16518Fluorescent Anti-IgGs抗小鼠4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug16900Streptavidin Conjugates其他生物染料1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug8C14708Signaling Intermediate Ab功能性抗体2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate12518Reporter Gene Enzymes其它检测试剂盒2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi1100mFluor™ Dyes and Kits标记试剂盒2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests10058Diagnostic Molecules定量试剂盒2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each38101cAMP GPCR Assays常规细胞株0.00
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔