Overview | ![]() PrinterFriendlyVersion |
Ex/Em(nm) | 518/605 |
MW | 315.41 |
CAS# | 104821-25-2 |
Solvent | DMSO |
Storage | F/D/L |
Category | EnzymeDetection RedoxEnzymes |
Related | CellSignalingMolecules ReactiveOxygenSpecies |
Spectrum | AdvancedSpectrumViewer |
5.1 Examinethefluorescenceofcell-freemixturesofdyeandbuffer/mediawithandwithouttheinducer.Intheabsenceofextracellularesterasesandotheroxidativeenzymes,thegradualincreaseinfluorescenceovertimemayberelatedtospontaneoushydrolysis,atmosphericoxidation,and/orlight-inducedoxidation.
5.2 Examinethefluorescenceofuntreated(control)loadedcellsthathavebeenmaintainedingrowthmediumorbuffer.Inhealthycells,oxygenrADIcalsareeliminatedbycellularenzymesand/ornaturalantioxidants.Followingthedye-loadingrecoveryperiod,healthycellsshouldexhibitalowleveloffluorescencethatisrelativelystableforthedurationoftheexperiment;however,agradualincrease(duetoauto-oxidation)ordecrease(duetolossofdyefromcellsorphotobleaching)influorescencemaybeobserved.Intheabsenceofanystimulusorinducement,aburstoffluorescenceinhealthy,untreatedcellscouldindicateprogresstocelldeathorsomeotheroxidativeevent.
References&Citations | ![]() CitationExplorer |
RoleofepigeneticregulationontheinductionofapoptosisinJurkatleukemiacellsbywhitegrapepomacerichinphenoliccompounds
Authors:AntonioJLeón-González,MJoséJara-Palacios,MalakAbbas,FJHeredia,ValérieSchini-Kerth
Journal:Food&Function(2017)
AstragalosideIVimprovestheisoproterenol-inducedvasculardysfunctionviaattenuatingeNOSuncoupling-mediatedoxidativestressandinhibitingROS-NF-$kappa$Bpathways
Authors:ChonghuaXu,FutianTang,MeiliLu,JingYang,RonghuiHan,MengMei,JinHu,MingshengZhou,HongxinWang
Journal:Internationalimmunopharmacology(2016):119--127
InhibitoryeffectofSophorasubprosratepolysaccharideonmitochondriaoxidativestressinducedbyPCV-2infectioninRAW264.7cells
Authors:Zi-JieSu,JianYang,Wen-JuanLuo,Ying-YiWei,Xue-HongShuai,Ting-JunHu
Journal:InternationalJournalofBIOLOGicalMacromolecules(2016)
EvaluationoftheMedicinalHerbGraptopetalumparaguayenseasaTreatmentforLiverCancer
Authors:Wei-HsiangHsu,Chia-ChuanChang,Kai-WenHuang,Yi-ChenChen,Shih-LanHsu,Li-ChenWu,Ann-PingTsou,Jin-MeiLai,Chi-YingFHuang
Journal:PloSone(2015):e0121298
SalvagingbrainischemiabyincreasingneuroprotectantuptakeviananoagoNISTmediatedbloodbrainbarrierpermeabilityenhancement
Authors:ShuyanZheng,Ying-YingBai,YikangLiu,XihuiGao,YanLi,YinzhiChangyi,YuanchengWang,DiChang,ShenghongJu,CongLi
Journal:Biomaterials(2015):9--20
PretreatmentwithAstragalosideIVprotectshumanumbilicalveinendothelialcellsfromhydrogenperoxideinducedoxidativestressandcelldysfunctionviainhibitingeNOSuncouplingandNADPHoxidase-ROS-NF-$kappa$Bpathway
Authors:ChonghuaXu,FutianTang,MeiliLu,JingYang,RonghuiHan,MengMei,JinHu,HongxinWang
Journal:CanadianJournalofPhysiologyandPharmacology
AAT Bioquest常用产品报价单
产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |