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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers*/235/100 mg
产品编号:235
市  场 价:¥27560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1378.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/CDCF [5-(and-6)-Carboxy-2',7'-dichlorofluorescein] *Mixed isomers*/235/100 mg
商品介绍
Overview
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Ex/Em(nm)504/529
MW445.21
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryEnzymeDetection
RedoxEnzymes
RelatedReactiveOxygenSpecies
CDCF[5(6)-carboxy-2",7"-dichlorofluorescein]isthefluorescentproductof5(6)-carboxy-2",7"-dichlorodihydrofluoresceindiacetateand5(6)-carboxy-2",7"-dichlorofluoresceindiacetateincells.Thecell-permeant5(6)-carboxy-2",7"-dichlorodihydrofluoresceindiacetate(H2DCFDA)(alsoknownasdichlorofluorescindiacetate)isachemicallyreducedformoffluoresceinusedasanindicatorforreactiveoxygenspecies(ROS)incells,forexampletodetectthegenerationofreactiveoxygenintermediatesinneutrophilsandmacrophages.Uponcleavageoftheacetategroupsbyintracellularesterasesandoxidation,thenon-fluorescentH2DCFDAisconvertedtothehighlyfluorescentCDCF.5(6)-carboxy-2",7"-dichlorofluoresceindiacetatestainingisusedforstudyingmultidrugresistanceproteins[MRPsandP-glycoprotein(Pgp)].
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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References&Citations
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1.   RemansPH,WijbrandtsCA,SandersME,ToesRE,BreedveldFC,TakPP,vanLaarJM,ReedquistKA.(2006)CTLA-4IGsuppressesreactiveoxygenspeciesbypreventingsynovialadherentcell-inducedinactivationofRap1,aRasfamilyGTPASEmediatorofoxidativestressinrheumatoidarthritisTcells.ArthritisRheum,54,3135.

2.   LiJJ,TangQ,LiY,HuBR,MingZY,FuQ,QianJQ,XiangJZ.(2006)Roleofoxidativestressintheapoptosisofhepatocellularcarcinomainducedbycombinationofarsenictrioxideandascorbicacid.ActaPharmacolSin,27,1078.

3.   ShabalaL,McMeekinT,BuddeBB,SiegumfeldtH.(2006)ListeriainnocuaandLactobacillusdelbrueckiisubsp.bulgaricusemploydifferentstrategiestocopewithacidstress.IntJFoodMicrobiol,110,1.

4.   TurncliffRZ,TianX,BrouwerKL.(2006)EffectofcultureconditionsontheexpressionandfunctionofBsep,Mrp2,andMdr1a/binsandwich-culturedrathepatocytes.BiochemPharmacol,71,1520.

5.   NezasaK,TianX,Zamek-GliszczynskiMJ,PatelNJ,RaubTJ,BrouwerKL.(2006)Alteredhepatobiliarydispositionof5(and6)-carboxy-2",7"-dichlorofluoresceininAbcg2(Bcrp1)andAbcc2(Mrp2)knockoutmice.DrugMetabDispos,34,718.

6.   StehfestE,TorkyA,GlahnF,FothH.(2006)Non-destructivemicromethodforMRP1functionalassayinhumanlungtumorcells.ArchToxicol,80,125.

7.   PrattS,ChenV,PerryWI,3rd,StarlingJJ,DantzigAH.(2006)KineticvalidationoftheuseofcarboxydichlorofluoresceinasadrugsurrogateforMRP5-mediatedtransport.EurJPharmSci,27,524.

8.   PenalvaMA.(2005)TracingtheendocyticpathwayofAspergillusnidulanswithFM4-64.FungalGenetBiol,42,963.

9.   BoldyrevAA.(2005)HomocysteinicacidcausesoxidativestressinlymphocytesbypotentiatingtoxiceffectofNMDA.BullExpBiolMed,140,33.

10.   MuzanduK,ShabanZ,IshizukaM,KazusakaA,FujitaS.(2005)Nitricoxideenhancescatecholestrogen-inducedoxidativestressinLNCaPcells.FreeRADIcRes,39,389.


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