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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Pro-AMC/13457/5 mg
产品编号:13457
市  场 价:¥27560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1378.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Pro-AMC/13457/5 mg
商品介绍
Overview
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Ex/Em (nm) 351/430
MW 272.30
CAS # N/A
Solvent DMSO
Storage F/D/L
Category Enzyme Detection
Peptidases and Proteases
Related Biochemical Assays
Pro-AMC is a sensitive fluorogenic protease substrate. The non-fluorescent substrate generates a bright blue fluorescent AMC product that has Ex/Em = 351/430 nm, and can be easily detected with the DAPI filter set. It has been used for monitoring the protease activity of tricorn protease (TRI), a high molecular mass complex from the archaeon T. acidophilum.
Spectrum Advanced Spectrum Viewer

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References & Citations
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1.   An L, Liu L, Wang S. (2009) Label-free, homogeneous, and fluorescence "turn-on" detection of protease using conjugated polyelectrolytes. Biomacromolecules, 10, 454.

2.   Bodenreider C, Beer D, Keller TH, Sonntag S, Wen D, Yap L, Yau YH, Shochat SG, Huang D, Zhou T, Caflisch A, Su XC, Ozawa K, Otting G, Vasudevan SG, Lescar J, Lim SP. (2009) A fluorescence quenching assay to discriminate between specific and nonspecific inhibitors of dengue virus protease. Anal Biochem, 395, 195.

3.   Doering K, Meder G, Hinnenberger M, Woelcke J, Mayr LM, Hassiepen U. (2009) A fluorescence lifetime-based assay for protease inhibitor profiling on human kallikrein 7. J Biomol Screen, 14, 1.

4.   Mizukami S, Takikawa R, Sugihara F, Shirakawa M, Kikuchi K. (2009) Dual-function probe to detect protease activity for fluorescence measurement and 19F MRI. Angew Chem Int Ed Engl, 48, 3641.

5.   Nakata H, Ohtsuki T, Sisido M. (2009) A protease inhibitor discovery method using fluorescence correlation spectroscopy with position-specific labeled protein substrates. Anal Biochem, 390, 121.

6.   Sabariegos R, Picazo F, Domingo B, Franco S, Martinez MA, Llopis J. (2009) Fluorescence resonance energy transfer-based assay for characterization of hepatitis C virus NS3-4A protease activity in live cells. Antimicrob Agents Chemother, 53, 728.

7.   Sheth RA, Upadhyay R, Stangenberg L, Sheth R, Weissleder R, Mahmood U. (2009) Improved detection of ovarian cancer metastases by intraoperative quantitative fluorescence protease imaging in a pre-clinical model. Gynecol Oncol, 112, 616.

8.   Tsai MT, Cheng YH, Liu YN, Liao NC, Lu WW, Kung SH. (2009) Real-time monitoring of human enterovirus (HEV)-infected cells and anti-HEV 3C protease potency by fluorescence resonance energy transfer. Antimicrob Agents Chemother, 53, 748.

9.   Wang X, Guo J, Wang J, Li S, Sun L, Lu J. (2009) [Prokaryotic expression and Identification of dual-fluorescence fusion proteins of small ubiquitin-like modifier and sentrin-specific protease]. Sheng Wu Gong Cheng Xue Bao, 25, 701.

10.   Zhang L, Xu Q, Xing D, Gao C, Xiong H. (2009) Real-time detection of caspase-3-like protease activation in vivo using fluorescence resonance energy transfer during plant programmed cell death induced by ultraviolet C overexposure. Plant Physiol, 150, 1773.


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