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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Signal Guard™ HRP reaction stopping solution/11020/0.5 ml
产品编号:11020
市  场 价:¥27560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$1378.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Signal Guard™ HRP reaction stopping solution/11020/0.5 ml
商品介绍
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Ex/Em(nm)None/None
MWN/A
CAS#N/A
SolventN/A
StorageF
CategoryEnzymeDetection
HorserADIshPeroxidase(HRP)
Related
HRPcouplingreactionsprovidesensitivebiomolecularassaysbasedonhydrogenperoxide-generatingenzymesystemslinkedtoperoxidase-mediatedoxidation.FluorogenicHRPsubstratesarepreferredtouseforenhancingassaysensitivities.Amongthem,themostcommonlyusedHRPsubstratesincludeADHP(alsocalledAmplex®Red,#11000),Amplex®UltraRedandAmplite™Red.Typically,detectionreactionsareperformedinmicroplatewellsandareinitiatedbyaddingafluorogenicHRPsubstrate,resultinginacontinuousfluorescenceincrease.Itiscriticaltoensurethatthetimingofthestandardandunknownsamplemeasurementsisthesame.OurSignalGuard™HRPreactionstoppingsolutionprovidesconvenienceandcontrolbyallowingthefluorescencesignal-generatingreactiontobeterminatedatauser-determinedtimepoint.Afteradditionofthestopreagent,thefluorescencesignalremainsstable.TheSignalGuard™HRPreactionstoppingsolutionisdesignedforuseinconjunctionwithADHP(Amplex®Red),Amplite™andAmplex®UltraRedfluorogenicsubstrates.Underthesameconditions,SignalGuard™HRPreactionstoppingsolutionsignificantlyoutperformstheAmplex®Red/UltraRedStopReagent(#A33855)fromThermofisher.OurSignalGuard™HRPreactionstoppingsolutioncanalsobeusedinotherHRPreactionsystems.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.     Make1XStopReagentbydilutionHRPReactionStoppingSolution20folds,e.g.,adding50µLofSignalGuard™HRPreactionstoppingsolutioninto950µLddH2O.

Note:Preparetheworkingsolutionsfreshlyasneeded,andavoidlight.

2.      Atthedesiredstoppingtimepoint,add20µLof1XStopReagentsolutionper100µLvolumeineachmicroplatewell.

 Note: Forotherreactionvolumes,adjusttheadditionof1XStopReagentproportionally(e.g.add5µLtoa25µLreactionvolume).The1Xstopreagentshouldbeaddedtoallwells,includinganyreagentcontrolswithoutHRP.Thetime-dependentfluorescencesignalincreasewillterminateimmediatelyandthefluorescencesignallevelshouldremainstableforatleast5hours.

Graph11020.jpg

Figure1.TheApplicationofHRPReactionStopSolutioninHRP-coupledglucosedetectionreaction.Twoparallelreactionscontaining15µMGlucosewereinitiatedbyadding50µLassaymixturecontaining:0.5mU/mLHRP,Amplex®Red,and0.5mU/mlGlucoseOxidase.Reactionswereincubatedatroomtemperaturefor5minsandthen20µL1XStopReagentwasaddedtoonereaction(A),and20µLddH2Ototheotherreaction(B).TheplotsdemonstratedthatthereactioniscompletelyinhibitedbySignalGuard™HRPReactionStoppingSolution.

References&Citations
CitationExplorer

AssessmentofTofacitinibandRuxolitinibandtheirAntiInflammatoryEffectsonMyeloperoxidase
Authors:AmberMilton
Journal:(2017)

PatternedPhotonicNitrocelluloseforPseudo-PaperELISA
Authors:JunjieChi,BingbingGao,MiSun,FenglingZhang,EnbenSu,HongLiu,ZhongzeGu
Journal:AnalyticalChemistry(2017)

SpinalCordInflammation:MolecularImagingafterThoracicAorticIschemiaReperfusionInjury
Authors:HassanAlbadawi,JohnWChen,RahmiOklu,YueWu,GregoryWojtkiewicz,BenjaminPulli,JohnDMilner,RichardPCambria,MichaelTWatkins
Journal:Radiology(2016):152222

MyeloperoxidaseNuclearImagingforEpileptogenesis
Authors:YinianZhang,DanielPSeeburg,BenjaminPulli,GregoryRWojtkiewicz,LionelBure,WendyAtkinson,StefanSchob,YoshikoIwamoto,MuhammadAli,WeiZhang
Journal:Radiology(2015):822--830

Myeloperoxidase--Hepatocyte--StellateCellCrossTalkPromotesHepatocyteInjuryandFibrosisinExperimentalNonalcoholicSteatohepatitis
Authors:BenjaminPulli,MuhammadAli,YoshikoIwamoto,MatthiasWGZeller,StefanSchob,JennyJLinnoila,JohnWChen
Journal:Antioxidants&redoxsignaling(2015):1255--1269

Orderedcleavageofmyeloperoxidaseesterbondsreleasesactivesitehemeleadingtoinactivationofmyeloperoxidasebybenzoicacidhydrazideanalogs
Authors:JianshengHuang,ForrestSmith,PeterPanizzi
Journal:Archivesofbiochemistryandbiophysics(2014):74--85

Raisingtheshields:PCRinthepresenceofmetallicsurfacesprotectedbytailor-madecoatings
Authors:FrankDScherag,ThomasBrandstetter,JürgenRühe
Journal:ColloidsandSurfacesB:Biointerfaces(2014):576--582

MeasuringmyeloperoxidaseactivityinBIOLOGicalsamples
Authors:BenjaminPulli,MuhammadAli,RezaForghani,StefanSchob,KevinLCHsieh,GregoryWojtkiewicz,JennyJLinnoila,JohnWChen
Journal:PLoSOne(2013):e67976

Micro-volumewall-lessimmunoassaysusingpatternedplanarplates
Authors:KatherineRKozak,JianyongWang,MelvinLye,RashiTakkar,NamyongKim,HyunjaeLee,NooLiJeon,KedanLin,CrystalZhang,WaiLeeTWong
Journal:LabonaChip(2013):1342--1350

Distinguishinginflammationfromtumorandperitumoraledemabymyeloperoxidasemagneticresonanceimaging
Authors:AnneKleijn,JohnWChen,JasonSBuhrman,GregoryRWojtkiewicz,YoshikoIwamoto,MartineLLamfers,AnatOStemmer-Rachamimov,SamuelDRabkin,RalphWeissleder,RobertLMartuza
Journal:ClinicalCancerResearch(2011):4484--4493


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