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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*/11003/1 L
产品编号:11003
市  场 价:¥143560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$7178.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/ReadiUse™ TMB Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*/11003/1 L
商品介绍
Overview
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Ex/Em(nm)650/None
MW240.34
CAS#54827-17-7
SolventN/A
StorageR/D/L
CategoryEnzymeDetection
HorserADIshPeroxidase(HRP)
RelatedBiochemicalAssays
Horseradishperoxidase(HRP)andHRPconjugatesfacilitatetheABTSoxidationinthepresenceofhydrogenperoxide,turningABTSintoitsblue-greenoxidizedproduct.ReadiUse™TMBSubstrateSolutionisapremixedsolutionofTMBsubstratewithhydrogenperoxide.ItproducesablueproductuponinteractionwithHRPorHRPconjugateswithouttheadditionofhydrogenperoxide.Thesolubleblueproductcanbequantitatedat650nm.Useofastopsolutionenhancessensitivity2-4foldandtheresultingyellowsolutioncanbereadat450nm.ReadiUse™TMBSubstrateSolutionprovidesanconvenientandultrasensitivequantitativesubstratesystem.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      WarmReadiUse™TMBSolutiontoroomtemperaturebeforeuse.

Note:Thereagentistobeusedassupplied,nodilutionisrequired.

 

2.       WashtheassayplatefollowingtheincubationofHRP-labeledreagent.

 

3.      Add100μLofReadiUseTMBSolutionintoeachwell.

 

4.      Incubatetheplateatroomtemperaturefor15–30minoruntilthedesiredcolordevelops.

Note:Theincubationtimevariesdependingontheassayconditions.

 

5.      Measuretheabsorbancesignalat650nmwithanELISAmicroplatereader.

References&Citations
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1.   LiuY,ZhuG,YangJ,YuanA,ShenX.(2014)Peroxidase-LikeCatalyticActivityofAg3PO4NanocrystalsPreparedbyaColloidalRoute.PLoSOne,9,e109158.

2.   JiangX,SunC,GuoY,NieG,XuL.(2014)Peroxidase-likeactivityofapoferritinpairedgoldclustersforglucosedetection.BiosensBioelectron,64C,165.

3.   LiuQ,YangY,LiH,ZhuR,ShaoQ,YangS,XuJ.(2014)NiOnanoparticlesmodifiedwith5,10,15,20-tetrakis(4-carboxylpheyl)-porphyrin:PromisingperoxidasemimeticsforHOandglucosedetection.BiosensBioelectron,64C,147.

4.   AumillerWM,Jr.,DavisBW,HatzakisE,KeatingCD.(2014)Interactionsofmacromolecularcrowdingagentsandcosoluteswithsmall-moleculesubstrates:effectonhorseradishperoxidaseactivitywithtwodifferentsubstrates.JPhysChemB,118,10624.

5.   LiuQ,JiaQ,ZhuR,ShaoQ,WangD,CuiP,GeJ.(2014)5,10,15,20-tetrakis(4-carboxylphenyl)porphyrin-CdSnanocompositeswithintrinsicperoxidase-likeactivityforglucosecolorimetricdetection.MaterSciEngCMaterBiolAppl,42,177.

6.   LinT,ZhongL,SongZ,GuoL,WuH,GuoQ,ChenY,FuF,ChenG.(2014)Visualdetectionofbloodglucosebasedonperoxidase-likeactivityofWS2nanosheets.BiosensBioelectron,62,302.

7.   PanY,LiN,MuJ,ZhouR,XuY,CuiD,WangY,ZhaoM.(2014)BiogenicmagneticnanoparticlesfromBurkholderiasp.YN01exhibitingintrinsicperoxidase-likeactivityandtheirapplications.ApplMicrobiolBiotechnol.

8.   MitraK,GhoshAB,SarkarA,SahaN,DuttaAK.(2014)Colorimetricestimationofhumanglucoselevelusinggamma-Fe(2)O(3)nanoparticles:aneasilyrecoverableeffectivemimicperoxidase.BiochemBiophysResCommun,451,30.

9.   LiuQ,LiH,ZhaoQ,ZhuR,YangY,JiaQ,BianB,ZhuoL.(2014)Glucose-sensitivecolorimetricsensorbasedonperoxidasemimicsactivityofporphyrin-Fe3O4nanocomposites.MaterSciEngCMaterBiolAppl,41,142.

10.   ChenX,TianX,SuB,HuangZ,OyamaM.(2014)Aunanoparticlesoncitrate-functionalizedgraphenenanosheetswithahighperoxidase-likeperformance.DaltonTrans,43,7449.


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