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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*/11001/1 L
产品编号:11001
市  场 价:¥50760.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2538.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/ReadiUse™ ABTS Substrate Solution *Optimized for ELISA Assays with HRP Conjugates*/11001/1 L
商品介绍
Overview
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Ex/Em(nm)420/None
MWN/A
CAS#30931-67-0
SolventN/A
StorageR/D/L
CategoryEnzymeDetection
HorserADIshPeroxidase(HRP)
RelatedBiochemicalAssays
Horseradishperoxidase(HRP)andHRPconjugatesfacilitatetheABTSoxidationinthepresenceofhydrogenperoxide,turningABTSintoitsblue-greenoxidizedproduct.ThischromogenicreactioniswidelyusedforquantifyHRPinELISAassays.TheoxidizedABTSproducthastheabsorptionmaximumof420nmthatcaneasilybefollowedwithaspectrophotometer.ReadiUse™ABTSSolutionisoptimizedforELISAassaysthatuseHRPorHRP-labeledconjugatesandhydrogenperoxideinmicrowellplatesortesttubes.OurABTSsolutionallowsHRPreactiondoneinasingleaddition.TheassaysolutionchangesitscolortolightgreenuponitsreactionwithHRPorHRPconjugatesinthepresenceofhydrogenperoxide.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      WarmReadiUse™ABTSSolutiontoroomtemperaturebeforeuse.

Note:Thereagentistobeusedassupplied,nodilutionisrequired.

 

2.       WashtheassayplatefollowingtheincubationofHRP-labeledreagent.

 

3.      Add100μLofReadiUseABTSSolutionintoeachwell.

 

4.      Incubatetheplateatroomtemperaturefor15–30min.

Note:Theincubationtimevariesdependingontheassayconditions.

 

5.      Measuretheabsorbancesignalat415+10nm(maximumat420nm)withanELISAmicroplatereader.

References&Citations
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1.   AmaralS,MiraL,NogueiraJM,daSilvaAP,HelenaFlorencioM.(2009)Plantextractswithanti-inflammatoryproperties--anewapproachforcharacterizationoftheirbioactivecompoundsandestablishmentofstructure-antioxidantactivityrelationships.BioorgMedChem,17,1876.

2.   ElbazJ,MosheM,ShlyahovskyB,WillnerI.(2009)Cooperativemulticomponentself-assemblyofnucleicacidstructuresfortheactivationofDNAzymecascades:aparadigmforDNAsensorsandaptasensors.Chemistry,15,3411.

3.   FreemanR,SharonE,Tel-VeredR,WillnerI.(2009)Supramolecularcocaine-aptamercomplexesactivatebiocatalyticcascades.JAmChemSoc,131,5028.

4.   PizzulL,CastilloMD,StenstromJ.(2009)Degradationofglyphosateandotherpesticidesbyligninolyticenzymes.Biodegradation.

5.   vanDongenSF,NallaniM,CornelissenJJ,NolteRJ,vanHestJC.(2009)Athree-enzymecascadereactionthroughpositionalassemblyofenzymesinapolymersomenanoreactor.Chemistry,15,1107.

6.   WilnerOI,ShimronS,WeizmannY,WangZG,WillnerI.(2009)Self-assemblyofenzymesonDNAscaffolds:enroutetobiocatalyticcascadesandthesynthesisofmetallicnanowires.NanoLett,9,2040.

7.   LloberaA,CadarsoVJ,DarderM,DominguezC,Fernandez-SanchezC.(2008)Full-fieldphotonicbiosensorsbasedontunablebio-dopedsol-gelglasses.LabChip,8,1185.

8.   OzyurekM,BektasogluB,GucluK,GungorN,ApakR.(2008)Simultaneoustotalantioxidantcapacityassayoflipophilicandhydrophilicantioxidantsinthesameacetone-watersolutioncontaining2%methyl-beta-cyclodextrinusingthecupricreducingantioxidantcapacity(CUPRAC)method.AnalChimActa,630,28.

9.   RyanBJ,O"FagainC.(2008)EffectsofmutationsinthehelixGregionofhorseradishperoxidase.Biochimie,90,1414.

10.   Duarte-VazquezMA,Garcia-PadillaS,Garcia-AlmendarezBE,WhitakerJR,RegaladoC.(2007)Broccoliprocessingwastesasasourceofperoxidase.JAgricFoodChem,55,10396.


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