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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Amplite™ Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*/10057/200 Tests
产品编号:10057
市  场 价:¥85560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$4278.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Amplite™ Fluorimetric Formaldehyde Quantitation Kit *Green Fluorescence*/10057/200 Tests
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)400/510
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategorySmallMoleculeDetection
DiagnosticMolecules
RelatedRedoxEnzymes
BiochemicalAssays
Formaldehydeisanaturallyoccurringsubstance.Naturalprocessesintheupperatmospheremaycontributeupto90percentofthetotalformaldehydeintheenvironment.Formaldehyde,aswellasitsoligomersandhydratesarerarelyencounteredinlivingorganisms.Methanogenesisproceedsviatheequivalentofformaldehyde,butthisone-carbonspeciesismaskedasamethylenegroupinmethanopterin.Formaldehydeistheprimarycauseofmethanol"stoxicity,sincemethanolismetabolizedintotoxicformaldehydebyalcoholdehydrogenase.OurAmplite™FluorimetricFormaldehydeQuantitationKitareusedforquantifyingformaldehyde.Thekitusesaproprietaryfluorogenicdyethatgeneratesastronglyfluorescentproductuponreactingwithformaldehyde.Thisfluorimetrickitprovidesasensitivemix-and-readmethodtodetectformaldehyde.Theassaycanbeperformedinaconvenient96-wellor384-wellmicrotiter-plateformatandeasilyadaptedtoautomationwithoutaseparationstep.ItssignalcanbeeasilyreadwithafluorescencemicroplatereaderatEx/Em=400/510nm.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Prepare500XAldeLight™Greenstocksolution:

Add20µLofDMSO(ComponentD)intotheAldeLight™Greenvial(ComponentA)tomake500Xstocksolution.

Note:TheunusedAldeLight™Greensolutionshouldbealiquoted,andstoredat-20oC(avoidlight).

 

2.PrepareAldeLight™Greenreactionmixture:

Add10µLof500XAldeLight™Green(fromStep1)into5mLofAssayBuffer(fromComponentB),mixwell.

Note:5mLofAldeLight™Greenreactionmixtureisenoughfor1plate.Thereactionmixtureisnotstable,andbestusedwithin2hours.

3.Prepareserialformaldehydestandard(0to300µM)solutions:

 

3.1   Add5µLof37.2%FormaldehydeStandard (ComponentD)into0.5mLofAssayBuffer(fromComponentB)tomake123mMstocksolution.

3.2    Add12.2μLof123mMFormaldehydeStandardSolution(fromStep3.1)into0.5mLofAssayBuffer(fromComponentB)tomake3mMstocksolution.

3.3   Take3mMFormaldehydeStandardSolution(fromStep3.2)toperform1:10,and1:3serialdilutionstoget 300,100,30,10,3,1,0.3,0.1,and0μMstandardformaldehydesolutions.

3.4   Addformaldehydestandardsandformaldehyde-containingtestsamplesintoa96-wellblacksolidmicroplateasdescribedinTables1and2

 

 

Table1.Layoutofformaldehydestandardsandtestsamplesinablacksolid96-wellmicroplate

BL

BL

TS

TS

….

….

 

 

 

 

 

 

FS1

FS1

….

….

….

….

 

 

 

 

 

 

FS2

FS2

 

 

 

 

 

 

 

 

 

 

FS3

FS3

 

 

 

 

 

 

 

 

 

 

FS4

FS4

 

 

 

 

 

 

 

 

 

 

FS5

FS5

 

 

 

 

 

 

 

 

 

 

FS6

FS6

 

 

 

 

 

 

 

 

 

 

FS7

FS7

 

 

 

 

 

 

 

 

 

 

Note:FS=FormaldehydeStandards,BL=BlankControl,TS=TestSamples.

 

Table2.Reagentcompositionforeachwell

FormaldehydeStandard

BlankControl

TestSample

Serialdilutions*(50μL)

Assaybuffer:50μL

50μL

*Note:Addtheseriallydilutedformaldehydestandardsfrom0.1μMto100μMintowellsfromFS1toFS7induplicate.

 

4.Runformaldehydeassay:

 

4.1 Add50μLofAldeLight™Greenreactionmixtures(fromStep2)toeachwelloftheformaldehydestandard,blankcontrol,andtestsamples(seeStep3.4)tomakethetotalformaldehydeassayvolumeof100µL/well.

Note:For384-wellplateadd25μLoftestsampleand25μLofAldeLight™Greenreactionmixturesintoeachwell.

4.2   Incubatethereactionmixtureatroomtemperaturefor20to60minutes(protectedfromlight).

4.3   MonitorthefluorescenceincreaseatEx/Em=410/525nmusingafluorescenceplatereader.

References&Citations
CitationExplorer

BIOLOGicalActivityofPeptide-conjugatedPolyionComplexMatricesConsistingofAlginateandChitosan
Authors:ChikaraFujimori,JunKumai,KyotaroNakamura,YingziGu,FumihikoKatagiri,KentaroHozumi,YamatoKikkawa,MotoyoshiNomizu
Journal:PeptideScience(2016)

HepaticDeficiencyofAugmenterofLiverRegenerationExacerbatesAlcohol-InducedLiverInjuryandPromotesFibrosisinMice
Authors:SudhirKumar,JiangWang,RichaRani,ChandrashekharRGandhi
Journal:PloSone(2016):e0147864

Integratedself-assemblingdrugdeliverysystempossessingdualresponsiveandactivetargetingfororthotopicovariancancertheranostics
Authors:Chun-JuiLin,Chen-HsiangKuan,Li-WenWang,Hsi-ChinWu,YunchingChen,Chien-WenChang,Rih-YangHuang,Tzu-WeiWang
Journal:Biomaterials(2016):12--26

Fiber-opticproteasesensorbasedonthedegradationofthingelatinfilms
Authors:BastienSchyrr,StéphanieBoder-Pasche,RéalIscher,RitaSmajda,GuyVoirin
Journal:SensingandBio-SensingResearch(2015):65--73


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