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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Cell Meter™ Colorimetric WST-8 Cell Quantification Kit/22770/1000 Tests
产品编号:22770
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Cell Meter™ Colorimetric WST-8 Cell Quantification Kit/22770/1000 Tests
商品介绍
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Ex/Em(nm)460/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryCellAnalysis
CellCytotoxicity
RelatedApoptosisandCytotoxicity
CellApoptosis
OurCellMeter™assaykitsareasetoftoolsformonitoringcellviABIlity.Thereareavarietyofparametersthatcanbeusedformonitoringcellviability.CellMeter™ColorimetricWST-8CellQuantificationKituseswater-solubleWST-8tetrazoliumsalttoquantifythenumberoflivecells.Thewater-solubleWST-8tetrazoliumsaltproducesawater-solubleorangeformazandyeuponbioreductioninthepresenceofanelectroncarrier,1-methoxy-5-methylphenaziniummethylsulfate.Thekitisconvenientandrobustwithamixandreadformat.WST-8solutionisaddeddirectlytothetestcells,nopre-mixingofcomponentsisrequired.WST-8tetrazoliumsaltisreducedbycellulardehydrogenasestoanorangeformazanproductthatissolubleintissueculturemedium.Theamountofformazanproducedisdirectlyproportionaltothenumberoflivingcellsbymonitoringabsorbanceincreaseat460nm.TheexcellentstabilityandlittlecytotoxicityofWST-8solutionmakethekitusefulfortheassaysthatrequirelongincubation(suchas24to48hours).CellMeter™ColorimetricWST-8CellQuantificationKitprovidesasensitivecolorimetricassayforthedeterminationofthenumberofviablecellsintheproliferationandcytotoxicityassays.Thedetectionsensitivityishigherthananyothertetrazoliumsalt-basedassayssuchasMTT,XTTorMTSetc.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. CellProliferationandCytotoxicityAssay
    1. Plate5000to10,000cells/wellinatissueculturemicroplatewithclearbottom.Addtestcompoundsintothecellsandincubateforadesiredperiodoftime(suchas24,48or96hours)ina37ºC,5%CO2incubator.Forblankwells(mediumwithoutthecells),addthesameamountoftestcompounds.Thesuggestedtotalvolumeis100μLfora96-wellplate,and50μLfora384-wellplate.
      Note:Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensityforproliferationorcytotoxicityinduction.Forproliferationassays,usefewercells;forcytotoxicityassays,usemorecellstostartwith.
    2. Add10μL/well(96-wellplate)or5μL/well(384-wellplate)ofWST-8Solutiontoeachwell.Incubatetheplateat37ºCfor1-4hours,andprotectfromlight.
      Note:Theincubationtimecouldbefrom30minutestoovernightdependingontheindividualcelltypeandcellconcentrationused.Optimizetheincubationtimeforeachexperiment.
    3. MonitortheabsorbanceincreasewithanabsorbanceplatereaderatOD=460nm.
  2. CellCountingAssay
    1. Preparecellcultureinatissueculturemicroplatewithclearbottom.Thesuggestedtotalvolumeis100μLfora96-wellplate,and50μLfora384-wellplate.
      Note:WeusedseriallydilutedHeLaandJurkatcellsUSPensioninaclearbottom96-wellplatefortheassay.SeeFigure2fordetails.
    2. Add10μL/well(96-wellplate)or5μL/well(384-wellplate)ofWST-8Solutiontoeachwell.Incubatetheplateat37ºCfor1-4hours,andprotectfromlight.
      Note:Theincubationtimecouldbefrom30minutestoovernightdependingontheindividualcelltypeandcellconcentrationused.Optimizetheincubationtimeforeachexperiment.
    3. MonitortheabsorbanceincreasewithanabsorbanceplatereaderatOD=460nm
References&Citations
CitationExplorer

miR-218inhibitstheproliferationofhumangliomacellsthroughdownregulationofYinYang1
Authors:YongGao,LaishengSun,ZichengWu,ChengminXuan,JunxiaZhang,YongpingYou,XinchengChen
Journal:MolecularMedicineReports(2017)


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