请使用支持JavaScript的浏览器!
主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Phalloidin-iFluor™ 790 Conjugate/23131/300 Tests
产品编号:23131
市  场 价:¥85560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$4278.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Phalloidin-iFluor™ 790 Conjugate/23131/300 Tests
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)787/808
MW~2800
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryCellBIOLOGy
LabelingCells
RelatedBiochemicalAssays
ThisinfraredfluorescentphalloidinconjugateselectivelybindstoF-actinswithmuchhigherphotostABIlitythanthefluorescein-phalloidinconjugates.Usedatnanomolarconcentrations,phalloidinderivativesareconvenientprobesforlabeling,identifyingandquantitatingF-actinsinformaldehyde-fixedandpermeabilizedtissuesections,cellculturesorcell-freeexperiments.Phalloidinbindstoactinfilamentsmuchmoretightlythantoactinmonomers,leADIngtoadecreaseintherateconstantforthedissociationofactinsubunitsfromfilamentends,essentiallystabilizingactinfilamentsthroughthepreventionoffilamentdepolymerization.Moreover,phalloidinisfoundtoinhibittheATPhydrolysisactivityofF-actin.Phalloidinfunctionsdifferentlyatvariousconcentrationsincells.Whenintroducedintothecytoplasmatlowconcentrations,phalloidinrecruitsthelesspolymerizedformsofcytoplasmicactinaswellasfilaminintostable"islands"ofaggregatedactinpolymers,yetitdoesnotinterferewithstressfibers,i.e.thickbundlesofMICROFILaments.ThepropertyofphalloidinisausefultoolforinvestigatingthedistributionofF-actinincellsbylabelingphalloidinwithfluorescentanalogsandusingthemtostainactinfilamentsforlightmicroscopy.Fluorescentderivativesofphalloidinhaveturnedouttobeenormouslyusefulinlocalizingactinfilamentsinlivingorfixedcellsaswellasforvisualizingindividualactinfilamentsinvitro.Fluorescentphalloidinderivativeshavebeenusedasanimportanttoolinthestudyofactinnetworksathighresolution.AATBioquestoffersavarietyoffluorescentphalloidinderivativeswithdifferentcolorsformulticolorimagingapplications.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
Sorry,yourbrowserdoesnotsupportinlineSVG.Sorry,yourbrowserdoesnotsupportinlineSVG.
Movemouseovergridtodisplaywavelength&intensityvalues.

300
400
500
600
700
800
900
Wavelength(nm)


Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.Prepare1000XPhalloidinDMSOstocksolution:byadding30uLofDMSOintothepowderformvials.

2.Prepare1XPhalloidinconjugateworkingsolution:byadding1uLof1000XPhalloidinconjugateDMSOsolutionto

1mLofPBSwith1%BSA.

Note1:Theunused1000XDMSOstocksolutionofphalloidinconjugateshouldbealiquotedandstoredat-20oC.protectedfromlight.

Note2:Differentcelltypesmightbestaineddifferently.Theconcentrationofphalloidinconjugateworkingsolutionshouldbepreparedaccordingly.

3.Stainthecells:

3.1   Performformaldehydefixation.Incubatecellswith3.0–4.0%formaldehydeinPBSatroomtemperaturefor10–30minutes.

Note:Avoidanymethanolcontainingfixativessincemethanolcandisruptactinduringthefixationprocess.Thepreferredfixativeismethanol-freeformaldehyde.

3.2   Rinsethefixedcells2–3timesinPBS.

3.3   Optional:Add0.1%TritonX-100inPBSintofixedcells(fromStep2.2)for3to5minutestoincreasepermeability.Rinsethecells2–3timesinPBS.

3.4   Add100uL/well(96-wellplate)ofphalloidinconjugateworkingsolution(fromStep1)intothefixedcells(fromStep2.2or2.3),andstainthecellsatroomtemperaturefor20to90minutes.

3.5   RinsecellsgentlywithPBS2to3timestoremoveexcessphalloidinconjugatebeforeplating,sealingandimagingundermicroscope.

References&Citations
CitationExplorer

BiomaterialSurfaceCanModifyHUVECMorphologyandInflammatoryResponsebyRegulatingMicroRNAExpression
Authors:ShuangyingGu,BaoxiangTian,WeicongChen,YueZhou
Journal:JournalofBiosciencesandMedicines(2017):8

Cell-Permeable,MMP-2Activatable,NickelFerriteandHis-taggedFusionProteinSelf-AssembledFluorescentNanoprobeforTumorMagneticTargetingandImaging
Authors:LuSun,ShupingXie,JingQi,ErgangLiu,DiLiu,QuanLiu,SunhuiChen,HuiningHe,VictorCYang
Journal:ACSAppliedMaterials&Interfaces(2017)

DNADouble-StrandBreaksInducetheNuclearActinFilamentsFormationinCumulus-EnclosedOocytesbutNotinDenudedOocytes
Authors:Ming-HongSun,MoYang,Feng-YunXie,WeiWang,LiliZhang,WeiShen,ShenYin,Jun-YuMa
Journal:PloSone(2017):e0170308

DNADouble-StrandBreaksInducetheNuclearActinFilamentsFormationinCumulus-EnclosedOocytesbutNotinDenudedOocytes
Authors:Ming-HongSun,MoYang,Feng-YunXie,WeiWang,LiliZhang,WeiShen,ShenYin,Jun-YuMa
Journal:PloSone(2017):e0170308

EnhancedbovineserumalbuminabsorptionontheN-hydroxysuccinimideactivatedgrapheneoxideanditscorrespondingcellaffinity
Authors:KunXiong,QingboFan,TingtingWu,HaishanShi,LinChen,MinhaoYan
Journal:MaterialsScienceandEngineering:C(2017)

Enhancedosteointegrationoftantalum-modifiedtitaniumimplantswithmicro/nano-topography
Authors:JunyuShi,XiaomengZhang,ShichongQiao,JieNi,JiajiMo,YingxinGu,HongchangLai
Journal:RSCAdvances(2017):46472--46479

GraftingofRing-OpenedCyclopropylaminethinfilmsonSilicon(100)HydrideviaUVPhotoionization
Authors:JolineTung,JingYuanChing,YokeMooiNg,LihShinTew,YitLungKhung
Journal:ACSAppliedMaterials&Interfaces(2017)

MicrotopographyAttenuatesEndothelialCellProliferationbyRegulatingMicroRNAs
Authors:DanWang,MengyaLiu,ShuangyingGu,YueZhou,SongLi
Journal:JournalofBiomaterialsandNanobiotechnology(2017):189--201

StudyontheRegulationofFocalAdesionsandCorticalActinbyMatrixNanotopographyin3DEnvironment
Authors:JingjingHan,Keng-huiLin,LockYueChew
Journal:JournalofPhysics:CondensedMatter(2017)

Thecorrelationbetweenosteopontinadsorptionandcelladhesiontomixedself-assembledmonolayersofvaryingchargesandwettability
Authors:LijingHao,TianjieLi,FanYang,NaruZhao,FuzhaiCui,XuetaoShi,ChangDu,YingjunWang
Journal:BiomaterialsScience(2017)


ViewMoreCitations
品牌介绍

AAT Bioquest常用产品报价单

产品名货号公司分类商城分类美金价
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg2iFluor Rapid Tests酸碱缓冲液1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug8B0001Signaling Intermediate Ab重组抗体2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug8A0022Phospho-Specific Ab受体2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug16440Fluorescent Anti-IgGs荧光染料1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug16518Fluorescent Anti-IgGs抗小鼠4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug16900Streptavidin Conjugates其他生物染料1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug8C14708Signaling Intermediate Ab功能性抗体2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate12518Reporter Gene Enzymes其它检测试剂盒2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi1100mFluor™ Dyes and Kits标记试剂盒2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests10058Diagnostic Molecules定量试剂盒2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each38101cAMP GPCR Assays常规细胞株0.00
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔