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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Cell Meter™ Fluorimetric Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry*/22841/100
产品编号:22841
市  场 价:¥3900.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$195.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Cell Meter™ Fluorimetric Cell Cycle Assay Kit *Green Fluorescence Optimized for Flow Cytometry*/22841/100
商品介绍
Overview
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Ex/Em(nm)503/526
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryCellAnalysis
CellCytotoxicity
RelatedApoptosisandCytotoxicity
CellApoptosis
BiochemicalAssays
OurCellMeter™assaykitsareasetoftoolsformonitoringcellviABIlityandproliferation.Thereareavarietyofparametersthatcanbeusedformonitoringcellviabilityandproliferation.Innormalcells,DNAdensitychangesdependingonwhetherthecellisgrowing,dividing,resting,orperformingitsordinaryfunctions.Theprogressionofthecellcycleiscontrolledbyacomplexinterplayamongvariouscellcycleregulators.Theseregulatorsactivatetranscriptionfactors,whichbindtoDNAandturnonorofftheproductionofproteinsthatresultincelldivision.Anymisstepinthisregulatorycascadecausesabnormalcellproliferationwhichunderliesmanypathologicalconditions,suchastumorformation.Potentialapplicationsforlive-cellstudiesareinthedeterminationofcellularDNAcontentandcellcycledistributionforthedetectionofvariationsingrowthpatterns,formonitoringapoptosis,andforevaluatingtumorcellbehaviorandsuppressorgenemechanisms.ThisparticularkitisdesignedtomonitorcellcycleprogressionandproliferationusingourproprietaryNuclearGreen™CCS1inlive,permeabilizedandfixedcells.ThepercentageofcellsinagivensamplethatareinG0/G1,SandG2/Mphases,aswellasthecellsinthesub-G1phasepriortoapoptosiscanbedeterminedbyflowcytometry.CellsstainedwithNuclearGreen™CC1canbemonitoredwithaflowcytometeratEx/Em=490nm/520nm(FL1channel).
SpectrumAdvancedSpectrumViewer

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.   Foreachsample,preparecellsin0.5mLofwarmmediumorbufferofyourchoiceatadensityof5×105to1×106cells/mL.

Note:Eachcelllineshouldbeevaluatedonanindividualbasistodeterminetheoptimalcelldensityforapoptosisinduction.

2.   Treatcellswithtestcompoundsforadesiredperiodoftimetoinduceapoptosisorothercellcyclefunctions.

 

3.   Add2.5μLof200XNuclearGreen™LCS1(ComponentA),andincubatethecellsina37°C,5%CO2incubatorfor30to60minutes.

Note1:Foradherentcells,gentlyliftthecellswith0.5mMEDTAtokeepthecellsintact,andwashthecellsoncewithserum-containingmediapriortoincubationwithNuclearGreen™LCS1.

Note2:Theappropriateincubationtimedependsontheindividualcelltypeandcellconcentrationused.Optimizetheincubationtimeforeachexperiment.

Note3:ItisnotnecessarytofixthecellsbeforeDNAstainingsincetheNuclearGreen™LCS1iscell-permeable.

 

4.    Optional:Centrifugethecellsat1000rpmfor4minutes,andthenre-sUSPendcellsin0.5mLofassaybuffer(ComponentB)orthebufferofyourchoice.

 

5.    MonitorthefluorescenceintensitybyflowcytometryusingtheFL1channel(Ex/Em=490/525nm).Gateonthecellsofinterest,excludingdebris.

References&Citations
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1.   FerulloDJ,CooperDL,MooreHR,LovettST.(2009)CellcyclesynchronizationofEscherichiacoliusingthestringentresponse,withfluorescencelabelingassaysforDNAcontentandreplication.Methods,48,8.

2.   EngstromJU,KmiecEB.(2008)DNAreplication,cellcycleprogressionandthetargetedgenerepairreaction.CellCycle,7,1402.

3.   KuoHM,ChangLS,LinYL,LuHF,YangJS,LeeJH,ChungJG.(2007)MorininhibitsthegrowthofhumanleukemiaHL-60cellsviacellcyclearrestandinductionofapoptosisthroughmitochondriadependentpathway.AnticancerRes,27,395.

4.   LouieMC,RevenkoAS,ZouJX,YaoJ,ChenHW.(2006)Directcontrolofcellcyclegeneexpressionbyproto-oncogeneproductACTR,anditsautoregulationunderliesitstransformingactivity.MolCellBiol,26,3810.

5.   EaswaranHP,LeonhardtH,CardosoMC.(2005)CellcycleMarkersforlivecellanalyses.CellCycle,4,453.

6.   EssersJ,vanCappellenWA,TheilAF,vanDrunenE,JaspersNG,HoeijmakersJH,WymanC,VermeulenW,KanaarR.(2005)Dynamicsofrelativechromosomepositionduringthecellcycle.MolBiolCell,16,769.

7.   LunaL,RolsethV,HildrestrandGA,OtterleiM,DantzerF,BjorasM,SeebergE.(2005)DynamicrelocalizationofhOGG1duringthecellcycleisdisruptedincellsharbouringthehOGG1-Cys326polymorphicvariant.NucleicAcidsRes,33,1813.

8.   Baran-MarszakF,FeuillardJ,NajjarI,LeClorennecC,BechetJM,Dusanter-FourtI,BornkammGW,RaphaelM,FagardR.(2004)DifferentialrolesofSTAT1alphaandSTAT1betainfludarabine-inducedcellcyclearrestandapoptosisinhumanBcells.Blood,104,2475.

9.   ConlonKA,ZharkovDO,BerriosM.(2004)Cellcycleregulationofthemurine8oxoguanineDNAglycosylase(mOGG1):mOGG1associateswithmicrotubulesduringinterphaseandmitosis.DNARepair(Amst),3,1601.

10.   NunezR,GarayN,VillafaneC,BrunoA,LindgrenV.(2004)DescriptionofaflowcytometryapproachbasedonSYBR-14stainingfortheassessmentofDNAcontent,cellcycleanalysis,andsortingoflivingnormalandneoplasticcells.ExpMolPathol,76,29.


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