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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*/22
产品编号:22827
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Cell Meter™ Annexin V Binding Apoptosis Assay Kit *Deep Red Fluorescence Optimized for Flow Cytometry*/22
商品介绍
Overview
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Ex/Em(nm)650/668
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryCellAnalysis
CellCytotoxicity
RelatedApoptosisandCytotoxicity
CellApoptosis
BiochemicalAssays
OurCellMeter™assaykitsareasetoftoolsformonitoringcellviABIlity.Thereareavarietyofparametersthatcanbeusedformonitoringcellviability.Thisparticularkitisdesignedtomonitorcellapoptosisthroughmeasuringthetranslocationofphosphatidylserine(PS).Inapoptosis,PSistransferredtotheouterleafletoftheplasmamembrane.Theappearanceofphosphatidylserineonthecellsurfaceisauniversalindicatoroftheinitial/intermediatestagesofcellapoptosisandcanbedetectedbeforemorphologicalchangescanbeobserved.ThiskitusesafluorescentAnnexinVthatspecificallybindsPS.AnnexinVconjugateshavebeendemonstratedtoselectivelybindPS.ThisparticularassaykitisoptimizedtomonitorcellapoptosisusingaflowcytometerwiththeCy5filterset.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.      Treatcellswithtestcompoundsforadesiredperiodoftime(4-6hoursforJurkatcellstreatedwithcamptothecin)toinduceapoptosis.

Note:AnnexinVflowcytometricanalysisonadherentcellsisnotroutinelytestedsincespecificmembranedamagemayoccurduringcelldetachmentorharvesting.However,methodsforutilizingAnnexinVforflowcytometryonadherentcelltypeshavebeenpreviouslyreportedbyCasiola-Rosenetal.andvanEngelendetal(seeRefs1and2).

2.      Centrifugethecellstoget1-5×105cells/tube.

3.      ResUSPendcellsin200μLofAssayBuffer(ComponentB).

4.      Add2μLofAnnexinV-iFluor™488(ComponentA)intothecells.

Optional:Add2µLof100XPropidiumIodide(ComponentC)fornecrosiscells.

5.      Incubateatroomtemperaturefor30to60minutes,protectedfromlight.

6.      Optional:add200to300μLofAssayBuffer(ComponentB)toincreasevolumebeforeanalyzingthecellswithaflowcytometer(SeeStep7).

7.      MonitorthefluorescenceintensityofAnnexinV-iFluor™488usingtheFL1channel(Ex/Em=490/525nm),andmeasurethecellviabilitywithpropidiumiodideusingtheFL2channel.

References&Citations
CitationExplorer

Insitupolymerizablehydrogelincorporatedwithspecificpathogen-freeporcineplatelet-richplasmaforthereconstructionofthecornealendothelium
Authors:Yung-KaiLin,RuchiSharma,HsuMa,Wen-ShyanChen,Chao-LingYao
Journal:JournaloftheTaiwanInstituteofChemicalEngineers(2017)

NovelregulationsofMEF2-A,MEF2-D,andCACNA1SinthefunctionalincompetenceofADIpose-derivedmesenchymalstemcellsbyinducedindoxylsulfateinchronickidneydisease
Authors:DuyenThiDo,NamNhutPhan,Chih-YangWang,ZhengdaSun,Yen-ChangLin
Journal:Cytotechnology(2016):2589--2604

Shearstress-inducedalterationofepithelialorganizationinhumanrenaltubularcells
Authors:DamienMaggiorani,RomainDissard,MarcyBelloy,Jean-SébastienSaulnier-Blache,AudreyCasemayou,LaureDucasse,SandraGrès,JulieBellière,CécileCaubet,Jean-LoupBascands
Journal:PloSone(2015):e0131416

TargetingaG-protein-coupledreceptoroverexpressedinendocrinetumorsbymagneticnanoparticlestoinducecelldeath
Authors:ClaireSanchez,DarineElHajjDiab,VincentConnord,PascalClerc,EtienneMeunier,BernardPipy,BrunoPayré,ReasmeyPTan,MichelGougeon,JulianCarrey
Journal:AcsNano(2014):1350--1363


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