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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Calcein Red™ AM/21900/1 mg
产品编号:21900
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Calcein Red™ AM/21900/1 mg
商品介绍
Overview
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Ex/Em(nm)560/574
MW1015.79
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryCellBIOLOGy
LabelingCells
RelatedViABIlityandProliferation
CellViability
MDRResearch
BiochemicalAssays
CalceinAMisoneofthemostpopularfluorescentprobesusedforlabelingandmonitoringcellularfunctionsoflivecells.However,thesinglecolorofCalceinAMmakesitimpossIBLetousethisvaluablereagentinthemulticolorapplications.Forexample,itisimpossibletouseCalceinAMincombinationofGFP-tranfactedcellsduetothesamecolortoGFP.ToaddressthiscolorlimitationofCalceinAM,wehavedevelopedCalceinOrange™,CalceinRed™andCalceinDeepRed™.ThesenewCalceinAManalogsenablethemulticolorlabelingandfunctionalanalysisoflivecellsincombinationwithCalceinAM.Non-fluorescentCalceinRed™AMcaneasilygetintolivecellsandhydrolyzestogeneratestronglyfluorescentCalceinRed™dye.CalceinRed™dyecanbemonitoredwiththecommonTRITC/Cy3filterset.AATBioquestoffersCalceinRed™asareferencedyetoCalceinRed™AM.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Movemouseovergridtodisplaywavelength&intensityvalues.

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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

AMestersarethenon-polarestersthatreADIlycrosslivecellmembranes,andrapidlyhydrolyzedbycellularesterasesinsidelivecells.AMestersarewidelyusedforloadingavarietyofpolarfluorescentprobesintolivecellsnon-invasively.However,cautionsmustbeexcisedwhenAMestersareusedsincetheyaresusceptibletohydrolysis,particularlyinsolution.Theyshouldbereconstitutedjustbeforeuseinhigh-quality,anhydrousdimethylsulfoxide(DMSO).DMSOstocksolutionsmaybestoreddesiccatedat‑20°Candprotectedfromlight.Undertheseconditions,AMestersshouldbestableforseveralmonths.

 

FollowingisourrecommendedprotocolforloadingAMestersintolivecells.Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

a)      Preparea2to5mMstocksolutionofAMestersinhigh-quality,anhydrousDMSO.ThenonionicdetergentPluronic®F-127issometimesusedtoincreasetheaqueoussolubilityofAMesters.

Note:Anequalvolumeof20%Pluronic®F-127solutioncanbeaddedtoDMSOstocksolutionsofcellviabilityindicatorbeforedilutingintotheloadingbuffer.ThefinalPluronic®F-127concentrationisabout0.02%.AvarietyofPluronic®F-127solutionscanbepurchasedfromAATBioquest.

Caution:Thelong-termstorageofAMestersinthepresenceofPluronic®F-127isnotrecommended.

b)      Onthedayoftheexperiment,eitherdissolvethesolidindicatorsofcellviabilityinDMSOorthawanaliquotoftheindicatorstocksolutionstoroomtemperature.Prepareaworkingsolutionof1to10µMinthebufferofyourchoice(suchasHanksandHepesbuffer).Formostofthecelllines,cellviabilityindicatorsatthefinalconcentrationrangingfrom4to5µMarerecommended.Theexactconcentrationofindicatorsrequiredforcellloadingmustbedeterminedempirically.

c)      Ifyourcellscontainingtheorganicanion-transports,probenecid(1–2.5mM)orsulfinpyrazone(0.1–0.25mM)maybeaddedtothecellmediumtoreduceleakageofthede-esterifiedindicators.IncubatecellswithAMestersatroomtemperatureor37°Cfor20minutestoonehour.

Note:Decreasingtheloadingtemperaturemightreducetheindicatorcompartmentalization.

d)      Washcellsincellviabilityindicator-freebuffer(containingananiontransporterinhibitor,ifapplicable)toremoveexcessprobes.

Note:Cellsampleswereimmediatelyanalyzedbyflowcytometrytodeterminetheaveragefluorescencepercellattimezerowithoutwashing.

e)      RuntheexperimentsatthedesiredEx/Emwavelengths(seeTable1).

References&Citations
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Functionalevidencethattheself-renewalgeneNANOGregulatesesophagealsquamouscancerdevelopment
Authors:DengLi,XiaocongXiang,FeiYang,DongqinXiao,KangLiu,ZhuChen,RuolanZhang,GangFeng
Journal:BiochemicalandBiophysicalResearchCommunications(2017)

NINJ2--Anovelregulatorofendothelialinflammationandactivation
Authors:JingjingWang,JingjingFa,PengyunWang,XinzhenJia,HuixinPeng,JingChen,YifanWang,ChenhuiWang,QiuyunChen,XinTu
Journal:CellularSignalling(2017)

ErythropoietinStimulatesEndothelialProgenitorCellstoInduceEndothelializationinanAneurysmNeckAfterCoilEmbolizationbyModulatingVascularEndothelialGrowthFactor
Authors:PeixiLiu,YingjieZhou,QingzhuAn,YayingSong,XiChen,Guo-YuanYang,WeiZhu
Journal:MEDICINE(2016):1--8

FlexibleEndoscopicSprayApplicationofRespiratoryEpithelialCellsasPlatformTechnologytoApplyCellsinTubularOrgans
Authors:AnjaLenaThiebes,ManuelArminReddemann,JohannesPalmer,ReinholdKneer,StefanJockenhoevel,ChristianGabrielCornelissen
Journal:TissueEngineeringPartC:Methods(2016):322--331

Influenceofhypothermiaandsubsequentrewarminguponleukocyte-endothelialinteractionsandexpressionofJunctional-Adhesion-MoleculesAandB
Authors:NicolaiVBogert,IsabellaWerner,AngelaKornberger,PatrickMeybohm,AntonMoritz,TillKeller,UlrichAStock,AndresBeiras-Fernandez
Journal:Scientificreports(2016)

Influenceofhypothermiaandsubsequentrewarminguponleukocyte-endothelialinteractionsandexpressionofJunctional-Adhesion-MoleculesAandB
Authors:NicolaiVBogert,IsabellaWerner,AngelaKornberger,PatrickMeybohm,AntonMoritz,TillKeller,UlrichAStock,AndresBeiras-Fernandez
Journal:Scientificreports(2016)

InhibitionofABCtransportproteinsbyoilsandsprocessaffectedwater
Authors:HattanAAlharbi,DavidMVSaunders,AhmedAl-Mousa,JaneAlcorn,AlbertoSPereira,JonathanWMartin,JohnPGiesy,SteveBWiseman
Journal:AquaticToxicology(2016):81--88

Rapidgenerationofcollagen-basedmicrotissuestostudycell--matrixinteractions
Authors:Marie-ElenaBrett,AlexandraLCrampton,DavidKWood
Journal:Technology(2016):1--8

ToxicokineticsandtoxicodynamicsofchlorpyrifosisalteredinembryosofJapanesemedakaexposedtooilsandsprocess-affectedwater:evidenceforinhibitionofP-glycoprotein
Authors:HattanAAlharbi,JaneAlcorn,AhmedAl-Mousa,JohnPGiesy,SteveBWiseman
Journal:JournalofAppliedToxicology(2016)

Sprayingrespiratoryepithelialcellstocoattissue-engineeredconstructs
Authors:AnjaLenaThiebes,StefanieAlbers,ChristianKlopsch,StefanJockenhoevel,ChristianGCornelissen
Journal:BioResearchopenaccess(2015):278--287


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品牌介绍

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