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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Protonex™ Red 600/21207/1 mg
产品编号:21207
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Protonex™ Red 600/21207/1 mg
商品介绍
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Ex/Em(nm)575/597
MW698.94
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryCellBIOLOGy
pHandIonIndicators
RelatedCellMetabolism
BiochemicalAssays
Protonex™ReddyedemonstratedpH-dependentfluorescence.UnlikemostoftheexistingfluorescentdyesthataremorefluorescentathigherpH,acidicconditionsenhancethefluorescenceofProtonex™Reddye.ThefluorescenceofProtonex™ReddyedramaticallyincreasesaspHdecreasesfromneutraltotheacidic.Thelackoffluorescenceoutsidethecelleliminatesthewashsteps.Protonex™Reddyeprovidesapowerfultooltomonitoracidiccellcompartmentssuchasendosomesandlysosomes.Protonex™Reddyeisnon-fluorescentoutsidethecells,butfluorescesbrightlyredinacidiccompartments(suchasphagosomes,lysosomesandendosomes).ThisProtonex™RedenablesthespecificdetectionofcellularacidiccompartmentswithreducedsignalvariABIlityandimprovedaccuracyforimagingorflowapplications.ItcanbealsousedformultiplexingcellularfunctionalanalysiswithgreendyessuchasGFP,Fluo-8,calcein,orFITC-labeledantibodies.Protonex™RedhasthespectralpropertiessimilartothoseofTexasRed,makingthecommonfiltersetofTexasRedreADIlyavailabletotheassaysofProtonex™Red.
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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

TreatcellsasdesiredbeforemakingtheProtonex™workingsolution.

 

1.      Preparea1to10mMProtonex™stocksolutioninDMSO.Make0.1to10µMworkingsolutionbydilutingtheDMSOstocksolutionintoHankssolutionwith20mMHepesbuffer(HHBS)orbufferofyourchoice.

2.      Treatcellsasdesired.

3.      IncubatethecellswithProtonex™workingsolutionfor15minto2hoursat37ºC.

4.      Replacethedye-loadingsolutionwithHHBSbuffer.

5.    Analyzethecellswithaproperfluorescenceinstrumentfittedwiththecorrectfilterset

References&Citations
CitationExplorer

PHD2isaregulatorforglycolyticreprogramminginmacrophages
Authors:AnnemarieGuentsch,AngelikaBeneke,LijaSwain,KatjaFarhat,ShunmugamNagarajan,BenWielockx,KaaminiRaithatha,JanDudek,PeterRehling,AnkeZieseniss
Journal:MolecularandCellularBiology(2016):MCB--00236


品牌介绍

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