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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/MycoLight™ Green JJ98/24000/100 ul
产品编号:24000
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
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AAT Bioquest/MycoLight™ Green JJ98/24000/100 ul
商品介绍
Overview
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Ex/Em(nm)484/504
MWN/A
CAS#N/A
SolventDMSO
StorageF/D/L
CategoryMicroBIOLOGy
FlowCytometry
RelatedFluorescenceImaging
BiochemicalAssays
MycoLight™GreenJJ98stainisanexcellentgreen-fluorescentnuclearandchromosomecounterstainthatispermeanttobothprokaryoticandeukaryoticcellmembranes.MycoLight™GreenJJ98stainhasahighaffinityforDNAandexhibitsenhancedfluorescenceuponbindingwithanexcitationmaximumclosetothe488nmargonlaserlineandfluorescenceemissionmaximumat~500nm.MycoLight™GreenJJ98stainisparticularlyusefulasanuclearcounterstainforbacterialassayssinceitstainsbothliveanddeadGram-positiveandGram-negativebacteria.ItisanexcellentreplacementforSTYO®9(STYO®isthetrademarkofInvitrogen).
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
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Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

Note1:Thefollowingprotocolcanbeadaptedformostcelltypes.Theseconditionsrequireadjustmentforeachcelltypeandexperimentalsystem.Growthmedium,celldensity,thepresenceofothercelltypesandfactorsmayinfluencestaining.Residualdetergentonglasswaremayalsoaffectstainingofmanyorganisms,andcausebrightlystainedmaterialtoappearinsolutionswithorwithoutcellspresent.

Note2:UseplastictubeswhendilutingMycoLightTMGreenJJ98andJJ99,becausethedilutedstainadherestoglass.Ingeneral,thebestresultsareobtainedinbuffersthatdonotcontainphosphate.

 

1.Adherentcellsinculturemaybestainedinsituoncoverslips.PelletcellsinsUSPensionbycentrifugationand

   resuspendinbufferedsaltsolutionorwater.

2.DilutetheMycoLightTMGreenJJ98orJJ99withnon-phosphatebuffersuchasHepesbufferorbufferofyourchoice.Add MycoLightTMGreenJJ98.UsingtheconcentrationslistedbelowTable1asaguideline.Ininitialexperiments, itmaybebesttotryseveraldyeconcentrationsovertheentiresuggestedrangetodeterminetheconcentrationthat  yieldsoptimalstaining.

 

Table1.SuggestedconditionsforstainingcellswithMycoLightTMGreenJJ98

 

Application

Concentration

StainingConditions

Bacterialcells

50nM–20μM

Vortextomix,thenincubatefor1–30minutes.

Eukaryoticcells

10nM–5μM

Incubatefor10–120minutes.

Microarrays

50nMinTEbuffer

Incubatefor5minutes,rinseandthendry.

 

3.Stainedeukaryoticcellsgenerallyshowdiffusecytoplasmicstainingaswellasnuclearstaining.Particularly

   MycoLightTMGreenJJ98andJJ99intensestainingofintranuclearbodiesarefrequentlyobserved.

References&Citations
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1.   WattsMR,JamesG,SultanaY,GinnAN,OuthredAC,KongF,VerweijJJ,IredellJR,ChenSC,LeeR.(2014)Aloop-mediatedisothermalamplification(LAMP)assayforStrongyloidesstercoralisinstoolthatusesavisualdetectionmethodwithSYTO-82fluorescentdye.AmJTropMedHyg,90,306.

2.   WeidnerJ,CassensU,GohdeW,WullenweberJ,GreveB.(2013)AnewtriplexrealtimePCRwhichdistinguishesbetweenMRSA,MSSA,andmecAcoagulasenegativestrainsbymeansofmeltingpointanalysisusingSYTO9.ClinLab,59,795.

3.   HubbardKS,GutIM,ScheelerSM,LymanME,McNuttPM.(2012)CompatibilityofSYTO13andHoechst33342forlongitudinalimagingofneuronviABIlityandcelldeath.BMCResNotes,5,437.

4.   LarrosaM,TruchadoP,EspinJC,Tomas-BarberanFA,AllendeA,Garcia-ConesaMT.(2012)EvaluationofPseudomonasaeruginosa(PAO1)adhesiontohumanalveolarepithelialcellsA549usingSYTO9dye.MolCellProbes,26,121.

5.   EischeidAC.(2011)SYTOdyesandEvaGreenoutperformSYBRGreeninreal-timePCR.BMCResNotes,4,263.

6.   WlodkowicD,SkommerJ,DarzynkiewiczZ.(2011)RapidquantificationofcellviabilityandapoptosisinB-celllymphomaculturesusingcyanineSYTOprobes.MethodsMolBiol,740,81.

7.   SeputieneV,VilkoicaiteA,ArmalyteJ,PavilonisA,SuziedelieneE.(2010)Detectionofmethicillin-resistantStaphylococcusaureususingdoubleduplexreal-timePCRanddyeSyto9.FoliaMicrobiol(Praha),55,502.

8.   UllalAJ,PisetskyDS,ReichCF,3rd.(2010)UseofSYTO13,afluorescentdyebindingnucleicacids,forthedetectionofmicroparticlesininvitrosystems.CytometryA,77,294.

9.   UdovichJA,BesselsenDG,GmitroAF.(2009)AssessmentofacridineorangeandSYTO16forinvivoimagingoftheperitonealtissuesinmice.JMicrosc,234,124.

10.   WlodkowicD,SkommerJ,FaleyS,DarzynkiewiczZ,CooperJM.(2009)DynamicanalysisofapoptosisusingcyanineSYTOprobes:fromclassicaltomicrofluidiccytometry.ExpCellRes,315,1706.


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