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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Cell Meter™ Colorimetric Antioxidant Activity Assay Kit/15900/200 Tests
产品编号:15900
市  场 价:¥85560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$4278.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Cell Meter™ Colorimetric Antioxidant Activity Assay Kit/15900/200 Tests
商品介绍
Overview
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Ex/Em(nm)405/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryNeuroBIOLOGy
ReactiveOxygenSpecies
RelatedCellSignaling
Reactiveoxygenspecies(ROS)areimportantincellsignalingandmaintainingphysiologicalcellmetabolism.ExcessivelevelofROSdamagescellularcomponents,whichlinkstonumerousdiseases,includingcancer,metabolicdisorders,neurodegenerativediseaseetc.Antioxidants,includingenzymes,macromoleculesandsmallmoleculesaregeneratedtocombattheROSinduceddamagesinthelivingorganisms.Quantitativeanalysisofantioxidantactivityinbodyfluids,tissuesandcellsprovidesimportantbiologicalinformation.OurAmplite™ColorimetricantioxidantassaykituseABTSancolorimetricindicatorofantioxidantactivitybasedontheobservationthattheABIlityofantioxidantstopreventtheoxidationofABTS(2,2"-azino-bis(3-ethylbenzothiazoline-6-sulfonicacid)toABTS+byHorserADIshPeroxidase(HRP)andHydrogenperoxide(H2O2).ABTS+,asolublechromogen,canbemonitoredspectrophotometricallyat405nm.
SpectrumAdvancedSpectrumViewer



Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. Preparestocksolutions:
    1. 2000XPeroxidasestocksolution:Add1mLofAssayBuffer(ComponentB)intothevialofHorseradishPeroxidase(ComponentA).
      Note:TheunusedHRPsolutionshouldbestoredinsingleusealiquotsat-20°C.
    2. 10mMTroloxstocksolution:Add1mlofAssayBuffer(ComponentB)intothevialofTrolox(ComponentD).
      Note:Theunused10mMTroloxstocksolutionshouldbedividedintosingleusealiquotsandstoredat-20°C.

  2. PrepareTroloxstandardsortestsamples:
    1. PrepareaTroloxstandardbydiluting100μLofthe10mMTroloxstocksolution(fromStep1.2)into400μLofAssayBuffer(ComponentB)tohave2mMTroloxstandardsolution.Andthenperform1:2serialdilutionstoget1,0.5,0.25,0.125,0.0625,0.0312and0.0156mMseriallydilutedTroloxstandardsolution.Anon-Troloxbufferisincludedasblankcontrol.

  3. Add10µlTroloxstandards(induplicate)or10µltestsamples(dilutethesamplesinAssayBuffertobringtheantioxidantlevelwithinthesamerangeasTrolox,ifnecessary),or10µlAssayBuffer(BlankControl)ineachwell.
    Table1:LayoutofTroloxstandardsandtestsamplesinaclear96-wellmicroplate
    BLBLTSTS........
    SD1SD1................
    SD2SD2
    SD3SD3
    SD4SD4
    SD5SD5
    SD6SD6
    SD7SD7
    Note:SD=Standards;BL=BlankControl;TS=TestSamples

    Table2:Reagentcompositionforeachwell
    TroloxStandardBlankControlTestSample
    Serialdilutions:10μLAssayBuffer:10μL10μL

  4. AddHRPworkingsolution:
    1. Add2.5uLof2000XHRP(fromStep1.1)into5mLassaybuffer(ComponentB)tomakelHRPworkingsolution.
    2. Add20µlofHRPworkingsolutionineachwell.

  5. Add150µlReadiUseTMABTSSubstrate(ComponentC)Solutiontoeachwell.
    Note:Avoidlightanduseinpolypropylenecontainers.Theunusedsolutioncanbestoredat4°C.

  6. Incubatefor5minutesatroomtemperature.
    Note:The5minutesincubationtimeisaguideline.Theincubationtimecanbechangedtoobtainsuitableabsorbance.

  7. Readtheabsorbanceat405nmusinganAbsorbancemicroplatereader.
References&Citations
CitationExplorer

Fluorescentreal-timequantitativemeasurementsofintracellularperoxynitritegenerationandinhibition
Authors:ZhenLuo,QinZhao,JixiangLiu,JinfangLiao,RuoguPeng,YuntingXi,ZhenjunDiwu
Journal:Analyticalbiochemistry(2017):44--48

GenesilencingofendothelialvonWillebrandFactorattenuatesangiotensinII-inducedendothelin-1expressioninporcineaorticendothelialcells
Authors:AnarDushpanova,SilviaAgostini,EnricaCiofini,ManuelaCabiati,ValentinaCasieri,MarcoMatteucci,SilviaDelRy,AldoClerico,SergioBerti,VincenzoLionetti
Journal:ScientificReports(2016)


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