请使用支持JavaScript的浏览器!
主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Di-4-ANEPPS *CAS#: 90134-00-2*/21498/5 mg
产品编号:21498
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Di-4-ANEPPS *CAS#: 90134-00-2*/21498/5 mg
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)497/705
MW480.66
CAS#90134-00-2
SolventDMSO
StorageF/D/L
CategoryIonChannels
MembranePotentials
RelatedNeurologicalStains
BiochemicalAssays
Potential-sensitiveANEPdyesarezwitterionicmolecule,exhibitingthemostconsistentpotentiometricresponseindifferentcellandtissuetypes.Theycanbeintroducedintocellsbydirectadditionofstocksolutiontocellculturemedium,byusingPluronic®F-127,orbyretrogradelabeling.ANEPdyesarenon-fluorescentuntilboundtomembranes,andtheirfluoresceresponsestoelectricalpotentialchangesintheirenvironment.ANEPdyesarefast-responseprobesthatoperatebymeansofachangeintheirelectronicstructure,andconsequentlytheirfluorescenceproperties,inresponsetoachangeinthesurroundingelectricfield.Theiropticalresponseissufficientlyfasttodetecttransient(millisecond)potentialchangesinexcitablecells,includingsingleneurons,cardiaccells,andintactbrains.However,themagnitudeoftheirpotential-dependentfluorescencechangeisoftensmall;fast-responseprobestypicallyshowa2-10%fluorescencechangeper100mV.FurThermore,thesedyesdisplayapotential-dependentshiftintheirexcitationspectra,thuspermittingthequantitationofmembranepotentialusingexcitationratiomeasurements.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
Sorry,yourbrowserdoesnotsupportinlineSVG.Sorry,yourbrowserdoesnotsupportinlineSVG.
Movemouseovergridtodisplaywavelength&intensityvalues.

300
400
500
600
700
800
900
Wavelength(nm)

References&Citations
PrinterFriendlyVersion

1.   LarsenAP,SciutoKJ,MorenoAP,PoelzingS.(2012)Thevoltage-sensitivedyedi-4-ANEPPSslowsconductionvelocityinisolatedguineapighearts.HeartRhythm,9,1493.

2.   BachtelAD,GrayRA,StohlmanJM,BourgeoisEB,PollardAE,RogersJM.(2011)Anovelapproachtodualexcitationratiometricopticalmappingofcardiacactionpotentialswithdi-4-ANEPPSusingpulsedLEDexcitation.IEEETransBiomedEng,58,2120.

3.   OsakaiT,SawadaJ,NagataniH.(2009)Potential-modulatedfluorescencespectroscopyofthemembranepotential-sensitivedyedi-4-ANEPPSatthe1,2-dichloroethane/waterinterface.AnalBioanalChem,395,1055.

4.   HardyME,PollardCE,SmallBG,Bridgland-TaylorM,WoodsAJ,ValentinJP,ABI-GergesN.(2009)Validationofavoltage-sensitivedye(di-4-ANEPPS)-basedmethodforassessingdrug-induceddelayedrepolarisationinbeagledogleftventricularmidmyocardialmyocytes.JPharmacolToxicolMethods,60,94.

5.   NovakovaM,BardonovaJ,ProvaznikI,TaborskaE,BochorakovaH,PaulovaH,HorkyD.(2008)Effectsofvoltagesensitivedyedi-4-ANEPPSonguineapigandrabbitmyocardium.GenPhysiolBiophys,27,45.

6.   XuZH,ZhangZX,WangJ,ZhangH,LiZ,JinYS,DingHY.(2007)[Spectralstudyofvoltagesensitivedyedi-4-ANEPPS].GuangPuXueYuGuangPuFenXi,27,1359.

7.   DumasJH,3rd,KnisleySB.(2005)Two-photonexcitationofdi-4-ANEPPSforopticalrecordingofactionpotentialsinrabbitheart.AnnBiomedEng,33,1802.

8.   TominagaT,TominagaY,YamadaH,MatsumotoG,IchikawaM.(2000)QuantificationofopticalsignalswithelectrophysiologicalsignalsinneuralactivitiesofDi-4-ANEPPSstainedrathippocampalslices.JNeurosciMethods,102,11.

9.   JohnsonPL,SmithW,BaynhamTC,KnisleySB.(1999)ErrorscausedbycombinationofDi-4ANEPPSandFluo3/4forsimultaneousmeasurementsoftransmembranepotentialsandintracellularcalcium.AnnBiomedEng,27,563.

10.   ChaloupkaR,PlasekJ,SlavikJ,SiglerovaV,SiglerK.(1997)MeasurementofmembranepotentialinSaccharomycescerevisiaebytheelectrochromicprobedi-4-ANEPPS:effectofintracellularprobedistribution.FoliaMicrobiol(Praha),42,451.


ViewAllAsPDF
品牌介绍

AAT Bioquest常用产品报价单

产品名货号公司分类商城分类美金价
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg2iFluor Rapid Tests酸碱缓冲液1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug8B0001Signaling Intermediate Ab重组抗体2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug8A0022Phospho-Specific Ab受体2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug16440Fluorescent Anti-IgGs荧光染料1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug16518Fluorescent Anti-IgGs抗小鼠4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug16900Streptavidin Conjugates其他生物染料1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug8C14708Signaling Intermediate Ab功能性抗体2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate12518Reporter Gene Enzymes其它检测试剂盒2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi1100mFluor™ Dyes and Kits标记试剂盒2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests10058Diagnostic Molecules定量试剂盒2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each38101cAMP GPCR Assays常规细胞株0.00
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔