![AAT Bioquest/DiIC12(3) perchlorate [1,1-Didodecyl-3,3,3,3-tetramethylindocarbocyanine perchlorate]/22035/25 mg](images/AAT201712/chemical-structure-for-diic12-3-perchlorate-1-1-didodecyl-3-3-3-3-tetramethylindocarbocyanine-perchlorate.png)
| Overview |  PrinterFriendlyVersion |
| Ex/Em(nm) | 549/565 |
| MW | 765.55 |
| CAS# | N/A |
| Solvent | DMSO |
| Storage | F/D/L |
| Category | CellBIOLOGy LabelingCells |
| Related | Lipoproteins VitalStains BiochemicalAssays |
| Spectrum | AdvancedSpectrumViewer |
1.PrepareDiO,DiIDiD,DiSorDiRmembranestainsolutions:
1.1 PrepareDMSOorEtOHstocksolutions:ThestocksolutionsshouldbepreparedinDMSOorEtOHat1-5mM.
Note:Theunusedportionofthestocksolutionshouldbestoredat-20oC.Avoidrepeatedfreeze/thawcycles.
1.2 Prepareworkingsolutions:Dilutethestocksolutions(fromStep1.1)intoasuitablebuffersuchasserum-freeculturemedium,HBSSorPBStomake1to5µMworkingsolutions.
Note:Thefinalconcentrationoftheworkingsolutionshouldbeempiricallydeterminedfordifferentcelltypesand/orexperimentalconditions.Itisrecommendedtotestattheconcentrationsthatareatleastoveratenfoldrange.
2.StainthecellsinsUSPension:
2.1 Suspendcellsatadensityof1×106/mLindyeworkingsolution(fromStep1.2).
2.2 Incubateat37°Cfor2–20minutes.Theoptimalincubationtimevariesdependingonthecelltype.Startbyincubatingfor20minutesandsubsequentlyoptimizeasnecessarytoobtainuniformlabeling.
2.3 Centrifugethelabeledsuspensiontubesat1000to1500rpmfor5minutes.
2.4 Removethesupernatantandgentlyresuspendthecellsinpre-warmed(37°C)growthmedium.
2.5 WashtwomoretimesasSteps2.3and2.4.
3.Stainadherentcells:
3.1 Growadherentcellsonsterileglasscoverslips.
3.2 Removecoverslipsfromgrowthmediumandgentlydrainoffexcessmedium.Placecoverslipinahumiditychamber.
3.3 Pipet100μLofthedyeworkingsolution(fromStep1.2)ontothecornerofacoverslipandgentlyagitateuntilallcellsarecovered.
3.4 Incubatethecoverslipat37°Cfor2–20minutes.Theoptimalincubationtimevariesdependingonthecelltype.Startbyincubatingfor20minutesandsubsequentlyoptimizeasnecessarytoobtainuniformlabeling.
3.5 Drainoffthedyeworkingsolutionandwashthecoverslipstwotothreetimeswithgrowthmedium.Foreachwashcycle,coverthecellswithpre-warmedgrowthmedium,incubatefor5-10minutesandthendrainoffthemedium.
4.MicroscopyDetection:
4.1 TheselectionofDiD,DiO,DiI,DiSandDiR’sfiltersetsissummarizedinTable1.
4.2 Forsimultaneousdetectionofmultipledyes,multibandfiltersetsareavailableasfollows:
a) DiIandDiO=OmegaXF52,Chroma51004
b) DiIandDiD=OmegaXF92,Chroma51007
c) DiI,DiOandDiD=OmegaXF93,Chroma61005
5.FlowCytometryDetection:
CellslabeledwithDiO,DiI,DiD,DiSandDiRcanbeanalyzedusingtheconventionalFL1,FL2,FL3andFL4flowcytometerdetectionchannels,respectively.
| References&Citations |  CitationExplorer |
CyclicRGDpeptide-modifiedLiposomaldrugdeliverysystemfortargetedoralapatinibadmiNISTration:enhancedcellularuptakeandimprovedtherapeuticeffects
Authors:ZhiwangSong,YunLin,ChanFengXiaZhang,YonglinLu,YongGao,ChunyanDong
Journal:InternationalJournalofNanomedicine(2017):1941
Invivoimagingsystemforexplantsanalysis—Anewapproachforassessmentofcelltransplantationeffectsinlargeanimalmodels
Authors:WeronikaZarychta-Wisniewska,AnnaBurdzinska,RadoslawZagozdzon,BartoszDybowski,MartaButrym,ZdzislawGajewski,LeszekPaczek
Journal:PloSone(2017):e0184588
InfluenceofParticleGeometryonGastrointestinalTransitandAbsorptionfollowingOralAdministration
Authors:DongLi,JieZhuang,HaishengHe,SifanJiang,AmritaBanerjee,YiLu,WeiWu,SamirMitragotri,LiGan,JianpingQi
Journal:ACSAppliedMaterials&Interfaces(2017)
Mesenchymalstemcellsincreaseskingraftsurvivaltimeandup-regulatePD-L1expressioninsplenocytesofmice
Authors:AliMoravej,BitaGeramizadeh,NegarAzarpira,Amir-HasanZarnani,RaminYaghobi,MehdiKalani,MaryamKhosravi,AminKouhpayeh,Mohammad-HosseinKarimi
Journal:ImmunologyLetters(2017)
Novelapproachforthedetectionofintraperitonealmicrometastasisusinganovariancancermousemodel
Authors:AyeshaBAlvero,DonginKim,EydisLima,NataliaJSumi,JungSeokLee,CarlosCardenas,MaryPitruzzello,Dan-ArinSilasi,NataliaBuza,TarekFahmy
Journal:ScientificReports(2017)
On-DemandDrugReleasingfromDualTargetingSmallNanoparticlesTriggeredbyHighIntensityFocusedUltrasoundEnhancedGlioblastomaTargetingTherapy
Authors:ZimiaoLuo,KaiJin,QiangPang,shunshen,ZhiqiangYan,TingJiang,XiaoyanZhu,LeiYu,ZhiqingPang,XinguoJiang
Journal:ACSAppliedMaterials&Interfaces(2017)
On-DemandDrugReleasingfromDualTargetingSmallNanoparticlesTriggeredbyHighIntensityFocusedUltrasoundEnhancedGlioblastomaTargetingTherapy
Authors:ZimiaoLuo,KaiJin,QiangPang,shunshen,ZhiqiangYan,TingJiang,XiaoyanZhu,LeiYu,ZhiqingPang,XinguoJiang
Journal:ACSAppliedMaterials&Interfaces(2017)
Overexpressionofc-Metinbonemarrowmesenchymalstemcellsimprovestheireffectivenessinhomingandrepairofacuteliverfailure
Authors:KunWang,YuwenLi,TiantianZhu,YongtingZhang,WentingLi,WenyuLin,JunLi,ChuanlongZhu
Journal:StemCellResearch&Therapy(2017):162
TheacceleratedbloodclearancephenomenonofPEGylatednanoemulsionuponcrossadministrationwithnanoemulsionsmodifiedwithpolyglycerin
Authors:YuqingSu,LirongWang,KaifanLiang,MengyangLiu,XinrongLiu,YanzhiSong,YihuiDeng
Journal:AsianJournalofPharmaceuticalSciences(2017)
Adualbrain-targetingcurcumin-loadedpolymersomesamelioratedcognitivedysfunctioninintrahippocampalamyloid-β1--42-injectedmice
Authors:TingtingJia,ZhiguoSun,YingLu,JieGao,HaoZou,FangyuanXie,GuoqingZhang,HaoXu,DuxinSun,YuanYu
Journal:InternationalJournalofNanomedicine(2016):3765
AAT Bioquest常用产品报价单
| 产品名 | 货号 | 公司分类 | 商城分类 | 美金价 |
| AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg | 2 | iFluor Rapid Tests | 酸碱缓冲液 | 1088.00 | AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug | 8B0001 | Signaling Intermediate Ab | 重组抗体 | 2828.00 | AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug | 8A0022 | Phospho-Specific Ab | 受体 | 2828.00 | AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug | 16440 | Fluorescent Anti-IgGs | 荧光染料 | 1088.00 | AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug | 16518 | Fluorescent Anti-IgGs | 抗小鼠 | 4278.00 | AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug | 16900 | Streptavidin Conjugates | 其他生物染料 | 1378.00 | AAT Bioquest/GCNT3 Antibody/8C14708/50 ug | 8C14708 | Signaling Intermediate Ab | 功能性抗体 | 2828.00 | AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate | 12518 | Reporter Gene Enzymes | 其它检测试剂盒 | 2103.00 | AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi | 1100 | mFluor™ Dyes and Kits | 标记试剂盒 | 2103.00 | AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests | 10058 | Diagnostic Molecules | 定量试剂盒 | 2828.00 | AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each | 38101 | cAMP GPCR Assays | 常规细胞株 | 0.00 |
本网站将在规定时间内给予删除等相关处理。
