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主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/ReadiUse™ NADPH Regenerating Kit/15265/1000 Tests
产品编号:15265
市  场 价:¥85560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$4278.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/ReadiUse™ NADPH Regenerating Kit/15265/1000 Tests
商品介绍
Overview
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Ex/Em(nm)None/None
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryCellBIOLOGy
CellMetabolism
RelatedRedoxEnzymes
BiochemicalAssays
NADPHprovidesthereducingequivalentsforbiosyntheticreactionsandforoxidation-reductioninvolvedinprotectionagainstthetoxicityofROS(reactiveoxygenspecies).NADPHisalsousedforanabolicpathways,suchaslipidsynthesis,cholesterolsynthesisandfattyacidchainelongation.Itisthesourceofreducingequivalentsforcytochrome450hydroxylationofaromaticcompounds,steroids,alcohols,anddrugs.NADPHisanecessarycofactorinmanyxenobioticmetabolismreactions.Inchloroplasts,NADPisreducedbyferredoxin-NADPreductaseinlaststepoftheelectronchainofthelightreactionsofphotosynthesis.TheNADPHproducedisthenusedasreducingpowerforthebiosyntheticreactionsintheCalvincycleofphotosynthesis.ManyoxidoreductasesandallligasesuseNADPHascoenzymes.NADPHisrequiredforthemeasurementofoxidaseactivitycatalyzedbyP450s,FMOs,NADPH-P450reductase,andmanyotheroxidaseenzymes.AATBioquest"sRediUse™NADPHRegeneratingKitprovidestwoready-to-usesolutionstoregenerateNADPHbyasimplemixing.ThiskitcanbeusedforallNADPH-requiringoxidaseassays(CDNA-expressedenzymesandliverfractions).About300-500enzymeassayscanbeperformedusingthiskit.Thetotalnumberofassaysthatcanbeperformeddependsonaresearcher"sexperimentaldesign.

Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.
  1. ThawComponentA,ComponentB,andComponentCatroomtemperaturebeforeuse.
  2. Make2XNADPHRegeneratingSolutionbyaddingthewholecontentofAssayBufferII(ComponentB)and500XGlucose-6-phosphatedehydrogenase(ComponentC)intoAssayBufferI(ComponentA),andmixthemwell.
  3. Addequalvolumeof2XNADPHRegeneratingSolution(fromStep2)intothedesiredassaysystem.
References&Citations
CitationExplorer

exo-Brevicominbiosynthesisinthefatbodyofthemountainpinebeetle,Dendroctonusponderosae
Authors:MinminSong,AndrewGorzalski,TrangTNguyen,XibeiLiu,ChristopherJeffrey,GaryJBlomquist,ClausTittiger
Journal:Journalofchemicalecology(2014):181--189

exo-BrevicominbiosyntheticpathwayenzymesfromtheMountainPineBeetle,Dendroctonusponderosae
Authors:MinminSong,PatrickDelaplain,TrangTNguyen,XibeiLiu,LeahWickenberg,ChristopherJeffrey,GaryJBlomquist,ClausTittiger
Journal:Insectbiochemistryandmolecularbiology(2014):73--80

FunctionalcharacterizationofmyrcenehydroxylasesfromtwogeographicallydistinctIpspinipopulations
Authors:MinminSong,AmyCKim,AndrewJGorzalski,MarinaMacLean,SharonYoung,MatthewDGinzel,GaryJBlomquist,ClausTittiger
Journal:Insectbiochemistryandmolecularbiology(2013):336--343

Aninsect-specificP450oxidativedecarbonylaseforcuticularhydrocarbonbiosynthesis
Authors:YueQiu,ClausTittiger,ClaudeWicker-Thomas,GaelleLeGoff,SharonYoung,EricWajnberg,ThierryFricaux,NathalieTaquet,GaryJBlomquist,RenéFeyereisen
Journal:ProceedingsoftheNationalAcademyofSciences(2012):14858--14863


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