请使用支持JavaScript的浏览器!
主营:研究并生产荧光和发光探针,信号转导研究的试剂
℡ 4000-520-616
℡ 4000-520-616
AAT Bioquest/Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*/11540/1000 Tes
产品编号:11540
市  场 价:¥56560.00
场      地:美国(厂家直采)
联系QQ:1570468124
电话号码:4000-520-616
邮      箱: info@ebiomall.com
美  元  价:$2828.00
品      牌: AAT Bioquest
公      司:AAT Bioquest
公司分类:
AAT Bioquest/Amplite™ Fluorimetric Goat Anti-Mouse IgG-HRP Conjugate ELISA Assay Kit *Red Fluorescence*/11540/1000 Tes
商品介绍
Overview
PrinterFriendlyVersion

Ex/Em(nm)571/585
MWN/A
CAS#N/A
SolventN/A
StorageF/D/L
CategoryEnzymeDetection
HorserADIshPeroxidase(HRP)
RelatedReactiveOxygenSpecies
MicroplateReaders
BiochemicalAssays
HorseradishPeroxidase(HRP)isasmallmolecule(MW~40KD)thatiswidelyusedinavarietyofBIOLOGicaldetections.HRPconjugatesareextensivelyusedassecondarydetectionreagentsinELISAs,immuno-histochemicaltechniques,Northern,SouthernandWesternblotanalyses.Duetoitssmallsize,itrarelycausessterichindranceproblemwithantibody/antigencomplexformation.Itisusuallyconjugatedtoanantibodyina4:1ratio.Additionally,HRPisinexpensivecomparedtootherlabelingenzymes.Themajordisadvantageassociatedwithperoxidaseistheirlowtolerancetomanypreservativessuchassodiumazidethatinactivatesperoxidaseactivityevenatlowconcentration.OurAmplite™FluorimetricELISAAssayKitcontainsalltheessentialcomponentsincludingourfluorogenicAmplite™RedHRPsubstrateforELISAdetection.ThekitprovidesanoptimizedassayprotocolthatiscompatIBLewithHTSliquidhandling,aslittleas10pgofamonoclonalantibody/wellofamicroplatecanbedetected.ItssignalcanbeeasilyreadbyeitherfluorescencemicroplatereaderwithEx/Em=530to570nm/590to600nmorabsorbancemicroplatereaderat~570nm.Itcanbeusedforassaysthatdetectgoatanti-mouseIgGasthesecondarydetectionagent.
SpectrumAdvancedSpectrumViewer

Sorry,yourbrowserdoesnotsupportinlineSVG.RelativeIntensity(%)100806040200Sorry,yourbrowserdoesnotsupportinlineSVG.
Sorry,yourbrowserdoesnotsupportinlineSVG.Sorry,yourbrowserdoesnotsupportinlineSVG.
Movemouseovergridtodisplaywavelength&intensityvalues.

300
400
500
600
700
800
900
Wavelength(nm)


Thisprotocolonlyprovidesaguideline,andshouldbemodifiedaccordingtoyourspecificneeds.

1.PrepareELISAplate:

1.1   PrepareELISAmicroplate(includingappropriatecontrols):PerformallnecessaryELISApreparationsteps.

 

1.2   Makegoatanti-mouseIgG-HRPconjugateworkingsolution:Add2µLofgoatanti-mouseIgG-HRPconjugate(ComponentE)to10mLofPBSwith1%BSA(PBS-BSA,notincluded).

Notes1:10mLofgoatanti-mouseIgG-HRPconjugateworkingsolutionisenoughfor1plate.Theconcentrationofthisgoatanti-mouseIgG-HRPconjugateworkingsolutionisrecommendedasaninitialconcentrationtotry;

2:Theoptimalconcentrationforeachparticularapplicationshouldbedeterminedempirically.

 

1.3   WashtheELISAwellsthreetimeswithPBScontaining0.02%to0.05%Tween®20(PBS-Tween)anddrain.

 

1.4   Add100μLofthedilutedHRPconjugateworkingsolution(fromStep1.2)intoeachwell(fromStep1.3)

 

1.5    Incubateatroomtemperaturefor30minutes.DrainofftheHRPconjugate.

 

1.6   WashthewellsthreetimeswithPBS-Tweenanddrain.

 

2.Preparestocksolutions:

2.1   200XAmplite™Redperoxidasesubstratestocksolution:Add250µLofDMSO(ComponentD)intothevialofAmplite™RedPeroxidaseSubstrate(ComponentA).Thestocksolutionshouldbeusedpromptly,andanyremainingsolutionshouldbealiquotedandrefrozenat-20oC.

Note:50µLoftheAmplite™Redperoxidasesubstratestocksolutionisenoughfor1plate.AliquotandstoreunusedDMSOstocksolutionat‑20oC.Avoidrepeatedfreeze-thawcycles.

 

2.2   20mMH2O2stocksolution:Add22.7µLof3%H2O2(0.88M,ComponentB)into977µLofAssayBuffer(ComponentC).

Note:ThedilutedH2O2stocksolutionisnotstable.Theunusedportionshouldbediscarded.

 

3.Prepareperoxidasereactionmixture:

PreparetheperoxidasereactionmixtureaccordingtoTable1andkeepfromlight.

 

Table1.ProxidaseReactionMixtureforOne96-wellPlate(1X)

Components

Volume

200XAmplite™Redperoxidasesubstratestocksolution(fromStep2.1)

50µL

20mMH2O2stocksolution(fromStep2.2)

100µL

Assaybuffer(ComponentC)

9.85mL

Totalvolume

10mL

 

4.RunperoxidaseassayinELISAplate:

4.1   Add100μLofperoxidasereactionmixture(fromStep3)intoeachdrainedmicroplatewellcontainingthesamplesandcontrols(fromStep1.6).

 

4.2   Incubatethereactionatroomtemperaturefor30minutesorlonger,protectedfromlight.

 

4.3   Monitorthefluorescenceincreasewithafluorescenceplatereaderatexcitation530-570nm(optimalat540nm)andemission590-600nm.

Note:Theplatecanalsobereadbyanabsorbancemicroplatereaderatthewavelengthof576±5nm.Theabsorptiondetectionhaslowersensitivitycomparedtofluorescencereading.

References&Citations
CitationExplorer

AssessmentofTofacitinibandRuxolitinibandtheirAntiInflammatoryEffectsonMyeloperoxidase
Authors:AmberMilton
Journal:(2017)

PatternedPhotonicNitrocelluloseforPseudo-PaperELISA
Authors:JunjieChi,BingbingGao,MiSun,FenglingZhang,EnbenSu,HongLiu,ZhongzeGu
Journal:AnalyticalChemistry(2017)

SpinalCordInflammation:MolecularImagingafterThoracicAorticIschemiaReperfusionInjury
Authors:HassanAlbadawi,JohnWChen,RahmiOklu,YueWu,GregoryWojtkiewicz,BenjaminPulli,JohnDMilner,RichardPCambria,MichaelTWatkins
Journal:Radiology(2016):152222

MyeloperoxidaseNuclearImagingforEpileptogenesis
Authors:YinianZhang,DanielPSeeburg,BenjaminPulli,GregoryRWojtkiewicz,LionelBure,WendyAtkinson,StefanSchob,YoshikoIwamoto,MuhammadAli,WeiZhang
Journal:Radiology(2015):822--830

Myeloperoxidase--Hepatocyte--StellateCellCrossTalkPromotesHepatocyteInjuryandFibrosisinExperimentalNonalcoholicSteatohepatitis
Authors:BenjaminPulli,MuhammadAli,YoshikoIwamoto,MatthiasWGZeller,StefanSchob,JennyJLinnoila,JohnWChen
Journal:Antioxidants&redoxsignaling(2015):1255--1269

Orderedcleavageofmyeloperoxidaseesterbondsreleasesactivesitehemeleadingtoinactivationofmyeloperoxidasebybenzoicacidhydrazideanalogs
Authors:JianshengHuang,ForrestSmith,PeterPanizzi
Journal:Archivesofbiochemistryandbiophysics(2014):74--85

Raisingtheshields:PCRinthepresenceofmetallicsurfacesprotectedbytailor-madecoatings
Authors:FrankDScherag,ThomasBrandstetter,JürgenRühe
Journal:ColloidsandSurfacesB:Biointerfaces(2014):576--582

Measuringmyeloperoxidaseactivityinbiologicalsamples
Authors:BenjaminPulli,MuhammadAli,RezaForghani,StefanSchob,KevinLCHsieh,GregoryWojtkiewicz,JennyJLinnoila,JohnWChen
Journal:PLoSOne(2013):e67976

Micro-volumewall-lessimmunoassaysusingpatternedplanarplates
Authors:KatherineRKozak,JianyongWang,MelvinLye,RashiTakkar,NamyongKim,HyunjaeLee,NooLiJeon,KedanLin,CrystalZhang,WaiLeeTWong
Journal:LabonaChip(2013):1342--1350

Distinguishinginflammationfromtumorandperitumoraledemabymyeloperoxidasemagneticresonanceimaging
Authors:AnneKleijn,JohnWChen,JasonSBuhrman,GregoryRWojtkiewicz,YoshikoIwamoto,MartineLLamfers,AnatOStemmer-Rachamimov,SamuelDRabkin,RalphWeissleder,RobertLMartuza
Journal:ClinicalCancerResearch(2011):4484--4493


品牌介绍

AAT Bioquest常用产品报价单

产品名货号公司分类商城分类美金价
AAT Bioquest/Fluorescein, disodium salt *CAS 518-47-8*/2/100 mg2iFluor Rapid Tests酸碱缓冲液1088.00
AAT Bioquest/14-3-3 ζ (Ab-58) Antibody/8B0001/50 ug8B0001Signaling Intermediate Ab重组抗体2828.00
AAT Bioquest/Opioid Receptor (Phospho-Ser375) Antibody/8A0022/50 ug8A0022Phospho-Specific Ab受体2828.00
AAT Bioquest/iFluor™ 350 goat anti-mouse IgG (H+L)/16440/200 ug16440Fluorescent Anti-IgGs荧光染料1088.00
AAT Bioquest/trFluor™ Eu goat anti-mouse IgG (H+L)/16518/100 ug16518Fluorescent Anti-IgGs抗小鼠4278.00
AAT Bioquest/RPE-streptavidin conjugate/16900/100 ug16900Streptavidin Conjugates其他生物染料1378.00
AAT Bioquest/GCNT3 Antibody/8C14708/50 ug8C14708Signaling Intermediate Ab功能性抗体2828.00
AAT Bioquest/Amplite™ Luciferase Reporter Gene Assay Kit *Bright Glow*/12518/1 plate12518Reporter Gene Enzymes其它检测试剂盒2103.00
AAT Bioquest/ReadiLink™ Rapid mFluor™ Violet 450 Antibody Labeling Kit *Microscale Optimized for Labeling 50 &mi1100mFluor™ Dyes and Kits标记试剂盒2103.00
AAT Bioquest/Amplite™ Colorimetric Urea Quantitation Kit *Blue Color*/10058/200 Tests10058Diagnostic Molecules定量试剂盒2828.00
AAT Bioquest/Screen Quest™ CHO-Gqi Chimera Cell line/38101/Each38101cAMP GPCR Assays常规细胞株0.00
自营商城图标
厂家直采
全球直采 正品优价
正品保障图标
正品保障
厂家直发 有线跟踪
解放采购图标
正规清关
CIF100%正规报关,提供发票
及时交付图标
及时交付
限时必达 不达必赔